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  • 1
    Electronic Resource
    Electronic Resource
    Intracellular Translocation of Myelin Proteolipid Protein (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 44 (1985), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Brainstem slices prepared from 22-day-old rats, were employed to study the intracellular translocation of radioactively labeled myelin proteolipid protein (PLP). Double-isotope and short pulse-chase procedures allowed us to demonstrate the flux of PLP through nine different subcellular membrane fractions that were isolated on the basis of their particle size and buoyant density. Tagged PLP was rapidly depleted from microsomes, showed transient passage through a number of presumably intermediate membranous pools, and accumulated in myelin. On the basis of the kinetics of PLP labeling and isotope ratios, the membranes can be arranged as they participate in the intracellular translocation of PLP and consistently show a pattern indicating possible precursor-product relationships.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1985.tb08788.x
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  • 2
    Electronic Resource
    Electronic Resource
    Protein composition of forebrain myelin isolated from triethyllead-intoxicated young rats (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 30 (1978), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1978.tb10801.x
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  • 3
    Electronic Resource
    Electronic Resource
    THE EFFECT OF TRIETHYLLEAD ON TOTAL AND MYELIN PROTEIN SYNTHESIS IN RAT FOREBRAIN SLICES (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 32 (1979), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Rats (20-day-old) were acutely intoxicated with triethyllead and their forebrains were studied during the following 14 days. All the lead in the tissue was found in the form of triethyllead, proving that the toxin per se was responsible for the pathological changes observed in the organ. The incorporation of [14C]leucine into the acid-insoluble protein was suppressed in the forebrain slices prepared from the intoxicated animals as well as in the slices, to which PbEt3 was added in vitro. In both systems the synthesis of myelin protein was inhibited more than the total protein synthesis. The results suggest a specificity of triethyllead toward processes involved in the furnishing of the myelin membrane proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1979.tb04526.x
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  • 4
    Electronic Resource
    Electronic Resource
    Suppressive Effect of Triethyllead on Entry of Proteins into the CNS Myelin Sheath In Vitro (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 35 (1980), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Incorporation of [14C]leucine into the myelin sheath was studied in brain stem slices prepared from 22-day-old rats. Individual major myelin proteins were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. There was a time lag before incorporation of the label into proteolipid protein (PLP) and intermediate protein (IP) reached maximal rates. Labelling of basic proteins (BP) and Wolfgram proteins (WP) revealed a much shorter lag in entry. Appearance of radioactive proteins in the myelin sheath was significantly hampered by triethyllead (PbEt3) added to the incubation medium at micromolar concentrations. Inhibition values were highest in the case of PLP and were closely followed by the values for IP. BP and WP were less inhibited, although incorporation of these proteins into myelin was still suppressed more than was synthesis of total homogenate protein. Thus, myelin-forming cells seem to be unduly vulnerable to the toxin relative to the rest of the tissue. Furthermore, the results indicate an interference of PbEt3 with certain posttranslational processes involved in furnishing of integral myelin proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1980.tb06275.x
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  • 5
    Electronic Resource
    Electronic Resource
    Colloquium C04: Toll-like receptors in the nervous system (2005)
    Oxford, UK : Blackwell Publishing Ltd.
    Journal of neurochemistry 94 (2005), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1474-1644.2005.03229_4.x
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  • 6
    Electronic Resource
    Electronic Resource
    ENDOGENOUS MICROSOMAL PHOSPHOLIPID PEROXIDATION IN MOUSE BRAIN (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 20 (1973), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Release of malondialdehyde from microsomal phospholipids during incubation in vitro was studied in the brain cytoplasm and in the microsomes fortified by deproteinized cytosole.Linearity between the log of specific activity of peroxidation (malondialdehyde release) and the negative log of protein concentration was demonstrated. The boiling of microsomes significantly quenched the ability to produce malondialdehyde, while preincubation with phospholipase A completely abolished this ability.In general, aromatic and not aliphatic compounds inhibited the reaction. 1,1,1-Trichloro-2,2-bis (p-chlorophenyl) ethane (DDT) was found to be a selective inhibitor of membrane peroxidative activity. The maximal inhibition is invariable. The data obtained revealed that brain and liver microsomal phospholipid peroxidation differ in some respects and the differences are of both quantitative and qualitative nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1973.tb00093.x
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  • 7
    Electronic Resource
    Electronic Resource
    Rapid isolation of genomic DNA from animal tissues
    Amsterdam : Elsevier
    Experimental Cell Research 190 (1990), S. 294-296 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(90)90200-T
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  • 8
    Electronic Resource
    Electronic Resource
    Enzymic and behavioural changes induced in mice fed polychlorinated biocides followed by starvation (1972)
    Springer
    Cellular and molecular life sciences 28 (1972), S. 902-903 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Gewicht, Gesamtprotein der Cytoplasmafraktion sowie die Aktivität des P-450-Komplexes der Leber wurden bei Mäusen nach Intoxikation mit DDT bzw. mit Lindan vor und während einer totalen Hungerperiode untersucht. Bei den hungernden Kontrolltieren war der Gesamtproteingehalt in der Cytoplasmafraktion der Leber niedrig. DDT und Lindan bewirkten eine Zunahme der Aktivität des P-450-Komplexes um 50% und 140%. Die Lindanintoxikation war stark ausgeprägt, so dass die betreffenden Tiere das Hungern nicht aushielten. Die hungernden, mit DDT vergifteten Mäuse wiesen eine weitere Steigerung der Aktivität des P-450-Komplexes auf; ihre Sterblichkeit betrug ca. 45%. Diese Tiere zeigten am Ende der Hungerperiode auch neurologische Störungen mit Einschränkungen der Bewegung.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01924934
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  • 9
    Electronic Resource
    Electronic Resource
    Turbidity as a measure of particulate subcellular fraction yield
    Amsterdam : Elsevier
    Journal of Biochemical and Biophysical Methods 23 (1991), S. 131-134 
    Details
    ISSN: 0165-022X
    Keywords: Myelin ; Subcellular fraction ; Turbidity measurement
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0165-022X(91)90060-A
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  • 10
    Electronic Resource
    Electronic Resource
    Improved sensitivity for detection and quantitation of glycoproteins on polyacrylamide gels (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 303-304 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A simple and sensitive technique for staining glycoproteins on polyacrylamide gels utilizing periodic acid-Schiff (PAS) reagent is described. The novelty of this technique is an additional incubation of the PAS stained gels in an ethanolic solution of metabisulfite. The treatment results in an about 20-fold increase in the intensitiy of bands and the color is stable for at least 1 week at room temperature. Furthermore, the integrated optical density of the bands is proportional to the amount of glycoproteins. Amounts as small as 0.2 μg of fetuin can be conveniently determined. As compared to other PAS staining methods, this procedure offers a remarkably improved sensitivity for detection and quantitation of glycoproteins following their electrophoretic separation on polyacrylamide gels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01947594
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