Keywords:
X-rays -- Scattering.
;
Small-angle x-ray scattering.
;
Electronic books.
Description / Table of Contents:
This book describes all aspects of the technique of small-angle scattering of X-rays and neutrons, including instrumentation, sample requirements, data interpretation and modelling methods, in a comprehensive way and gives examples of applications in various fields of biophysics and biochemistry.
Type of Medium:
Online Resource
Pages:
1 online resource (369 pages)
Edition:
1st ed.
ISBN:
9780191507038
Series Statement:
International Union of Crystallography Texts on Crystallography Series ; v.19
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=3055516
DDC:
572.33
Language:
English
Note:
Cover -- Contents -- Introduction -- References -- PART I: THEORY AND EXPERIMENT -- 1 Basics of small angle scattering -- 1.1 Elastic scattering of X-rays and neutrons -- 1.2 Scattering by macromolecular solutions -- 1.3 Resolution and contrast -- 1.4 Absorption and anomalous X-ray scattering -- References -- 2 X-ray and neutron scattering instruments -- 2.1 Characteristics of sources -- 2.2 X-ray sources -- 2.3 Neutron sources -- 2.4 SAXS instruments -- 2.5 SANS instruments -- 2.6 Special instruments -- References -- 3 Experimental practice and data processing -- 3.1 Sample requirements for SAXS and SANS -- 3.2 Experimental protocols and instrumental corrections -- 3.3 Special features of SAXS: radiation damage -- 3.4 Special features of SANS: the use of H[Sub(2)]O/D[Sub(2)]O mixtures -- 3.5 Random and systematic errors -- 3.6 Basic structural information and data quality -- 3.7 Calibration to absolute scale and molecular mass -- References -- PART II: DATA ANALYSIS METHODS -- 4 Monodisperse systems -- 4.1 Overall parameters of particles -- 4.2 Multipole representation of SAS intensity -- 4.3 Shannon sampling -- 4.4 Ab initio shape analysis -- 4.5 Computation of scattering patterns from atomic models -- 4.6 Hybrid methods -- 4.7 Labelling and triangulation -- References -- 5 Polydisperse and interacting systems -- 5.1 Size, shape and conformation polydispersity -- 5.2 Size distribution functions -- 5.3 Shape polydispersity and oligomeric mixtures -- 5.4 Conformational polydispersity and flexible systems -- 5.5 Interacting systems and structure factor -- References -- PART III: BIOLOGICAL APPLICATIONS OF SOLUTION SAS -- 6 Static structural studies -- 6.1 Applications of ab initio shape determination -- 6.2 Quaternary structure analysis of proteins and complexes -- 6.3 Equilibrium mixtures and oligomeric composition.
,
6.4 Membrane proteins and lipoproteins -- 6.5 Flexible systems -- References -- 7 Kinetic and perturbation studies -- 7.1 Dynamics and kinetics -- 7.2 Perturbation methods -- 7.3 Temperature scans and T-jumps -- 7.4 High-pressure experiments -- 7.5 Stopped-flow and continuous-flow mixing -- 7.6 Light-triggered processes -- References -- 8 Analysis of interparticle interactions -- 8.1 Basic physical chemistry of interactions -- 8.2 Experimental SAS studies of protein-protein interactions -- 8.3 Structure factor calculations for proteins -- 8.4 Interactions in nucleic acids -- References -- 9 SAS in multidisciplinary studies -- 9.1 Automation and high-throughput SAS -- 9.2 Joint use with high-resolution methods -- 9.3 SAS and low-resolution crystallography -- 9.4 Complementary biophysical methods -- 9.5 Bioinformatics and model validation -- References -- Conclusions and future prospects -- Appendices -- Appendix 1: Basic physics and mathematics of wave phenomena -- Appendix 2: Spherical harmonics and their applications for SAS -- Appendix 3: Interactions between spherical molecules -- Appendix 4: Web resources -- Abbreviations list -- Index of computer programs -- A -- B -- C -- D -- E -- F -- G -- H -- I -- M -- O -- P -- S -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- Z.
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