GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    Prevalence and cultivation of a Chelonid herpesvirus associated with Fibropapillomas of the green turtle, Chelonia mydas, and the loggerhead turtle, Caretta caretta, in Florida. (2021)
    Florida Cooperative Fish and Wildlife Research Unit , University of Florida | Gainesville, FL
    In:  http://aquaticcommons.org/id/eprint/1092 | 3 | 2011-09-29 21:09:23 | 1092 | Florida Cooperative Fish and Wildlife Research Unit
    Details
    Publication Date: 2021-07-05
    Description: Fibropapillomatosis (FP) is a growing threat to the survival of green turtle,Chelonia mydas, populations worldwide and has been shown to be affectingloggerhead, Caretta caretta, and olive ridley, Lepidochelys olivacea, populations aswell. Transmission studies in captivity carried out by this laboratory have demonstratedthat FP in green turtles is caused by an infectious subcellular agent. (Herbst, et al,1995), (Herbst et ai, 1996a). We have demonstrated a new Chelonid herpesvirus inexperimentally induced and spontaneous fibropapillomas of green turtles using electronmicroscopic,molecular, and serological techniques (Jacobson et ai, 1991), (Herbst, etai, 1995), (Herbst, et ai, 1996b), (Lackovich, et al 1998a), (Herbst, et ai, 1998), (Garber,et al 1998). Recent studies by others have confirmed our early characterization of thisvirus as a member of the Alphaherpesvirinae subfamily (Quackenbush, et al, 1998,Herbst, et al, 1996b, Garber, et ai, 1998).Although this herpesvirus is considered a candidate for the etiology of FP, it hasnot yet been cultivated in vitro. Fulfillment of Koch's postulates through a controlledtransmission study using cultured herpesvirus would test its role as the etiologic agentof FP. These studies were initiated to provide previously unavailable information aboutthe prevalence of this virus in tumors and skin of green and loggerhead turtles in Floridaand to attempt to cultivate it in vitro. (13 page document)
    Description: Research Work Order no. 161
    Keywords: Health ; Biology ; Green turtle ; Chelonia mydas ; fibropapillomatosis ; diseases ; Florida ; loggerhead turtle ; Caretta caretta ; marine turtle
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 2
    facet.materialart.
    Unknown
    Pathogenic, Molecular, and Immunological Properties of a Virus Associated with Sea Turtle Fibropapillomatosis. Phase II : Viral Pathogenesis and Development of Diagnostic Assays (2021)
    Florida Cooperative Fish and Wildlife Research Unit , University of Florida | Gainesville, FL
    In:  http://aquaticcommons.org/id/eprint/1093 | 3 | 2011-09-29 21:09:17 | 1093 | Florida Cooperative Fish and Wildlife Research Unit
    Details
    Publication Date: 2021-07-05
    Description: Research conducted under this RWO from July 1, 1997 through June 30, 2000 hasprovided important new information about the pathogenesis, virology, andimmunology of marine turtle fibropapillomatosis. In particular, we have providedstrong evidence for the association of a herpesvirus with fibropapillomatosis of thegreen turtle,Chelonia mydas, and the loggerhead turtle, Caretta caretta, in Florida. Inaddition we have provided new evidence for the absence of papillomaviruses fromsea turtle fibropapillomas. Although unsuccessful, important new attempts were madeto cultivate the FP-associated herpesvirus in vitro in collaboration with the NationalWildlife Health Center. During this period of time, we completed publication of the firstcomprehensive description of the comparative pathology and pathogenesis ofexperimentally induced and spontaneous fibropapillomas of green turtles (Cheloniamydas). We initiated innovative studies on the persistence of a Chelonianherpesviruses in the marine environment demonstrating for the first time that theenvironmental survivability of Chelonian herpesviruses makes them real threats tomarine turtle health. Finally, we explored development of a serological assay for FPusing synthetic herpesvirus peptides and developed methodologies for detection ofantibodies to LETV [Iung-eye-trachea virus] a disease-associated herpesvirus of thegreen turtle, Chelonia mydas.. This last initiative is ongoing and will further our effortsto develop specific immunological assays for the FP-associated herpesvirus and FP. (17 page document)
    Description: Research Work Order no. 180
    Keywords: Health ; Biology ; Green turtle ; Chelonia mydas ; fibropapillomatosis ; diseases ; Florida ; Loggerhead turle ; Caretta caretta ; marine turtles ; diagnostic assays
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 3
    facet.materialart.
    Unknown
    Seroepidemiological studies of herpesvirus-associated diseases of marine turtles: Fibropapillomatosis and lung-eye-trachea disease (2021)
    Florida Cooperative Fish and Wildlife Research Unit , University of Florida | Gainesville, FL
    In:  http://aquaticcommons.org/id/eprint/1089 | 3 | 2011-09-29 21:10:29 | 1089 | Florida Cooperative Fish and Wildlife Research Unit
    Details
    Publication Date: 2021-07-05
    Description: We have developed immunological tests that can identify marine turtles in Florida (green and loggerhead) that have been exposedto the LETV herpesvirus. The seroepidemiological data collected provides critical evidence about the relationship betweeninfection with the FP-associated herpesvirus and the LETV herpesvirus. The data supports the hypothesis that LETV and FPHVinfections are independent infections of marine turtles. The data shows that wild green turtles in Florida are exposed to theLETD-associated herpesvirus, which is the first description ofLETV infection in free-ranging marine turtles. To our knowledge,the antigenic proteins identified in this study are not only the first proteins from a reptilian herpesvirus to be cloned andexpressed, but they represent the first reptilian herpesvirus proteins to be identified as immunogenic in their host species. (16 page document)
    Description: Research Work Order no. 213
    Description: Project status report.
    Keywords: Health ; Biology ; Green turtle ; Chelonia mydas ; fibropapillomatosis ; diseases ; Florida ; loggerhead turtle ; Caretta caretta ; marine turtle ; lung-eye-trachea disease ; herpesviruses seroepidemiology ; recombinant-viral antigens ; ELISA
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 4
    facet.materialart.
    Unknown
    Further strategies for evaluating the etiological role of a tumor-associated herpesvirus in marine turtle fibropapillomatosis (2021)
    Florida Cooperative Fish and Wildlife Research Unit , University of Florida | Gainesville, FL
    In:  http://aquaticcommons.org/id/eprint/1088 | 3 | 2011-09-29 21:10:25 | 1088 | Florida Cooperative Fish and Wildlife Research Unit
    Details
    Publication Date: 2021-07-05
    Description: In 1992, an interdisciplinary research team headquartered at the University of Floridabegan studies in key targeted areas of fibropapillomatosis (FP) etiology andpathogenesis. At that time, little was known about FP outside of field studiesdocumenting its prevalence in different areas of the world and studies of tumorhistopathology. Our primary objective was to develop a broad-based scientificunderstanding of FP by applying principles of tumor biology, immunology, pathology,virology, molecular biology, and epidemiology to FP in the green turtle, Cheloniamydas. Long-term goals included the development of assays for FP and study of anyrole of environmental co-factors in the disease. This report is a continuation of thateffort and the results reported here bring us closer to understanding the role of atumor-associated herpesvirus in marine turtle fibropapillomatosis. This research has demonstrated that marine turtle herpesviruses can persist forextended periods of time as infectious agents in the marine environment and that wildgreen turtles in Florida are exposed to the LETD-associated herpesvirus. This is thefirst description of LETV infection in free-ranging. marine turtles. In addition, data ispresented that supports the hypothesis that LETV and FPHV infections areindependent. These data reveal new levels of complexity that must be addressedbefore reliable serodiagnostic assays for herpesvirus infections of chelonians can bedeveloped for widespread application. The results reported here also raise newconcerns about the potential impact of infections by new herpesviruses on populationsof wild marine turtles, an area which has previously been unexplored by turtlebiologists. (8 page document)
    Description: Research Work Order no. 194
    Keywords: Health ; Biology ; Green turtle ; Chelonia mydas ; fibropapillomatosis ; diseases ; Florida
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 5
    facet.materialart.
    Unknown
    Immunological competence in the green turtle and its relationship to the development of fibropapilloma disease (2021)
    Florida Cooperative Fish and Wildlife Research Unit , University of Florida | Gainesville, FL
    In:  http://aquaticcommons.org/id/eprint/1091 | 3 | 2011-09-29 21:09:20 | 1091 | Florida Cooperative Fish and Wildlife Research Unit
    Details
    Publication Date: 2021-07-05
    Description: Fibropapillomatosis (FP) remains a growing threat to the survival of sea turtles inFlorida and other locations worldwide. FP has seriously affected green turtle,Chelonia mydas, populations in Florida and Hawaii and is now emerging as asignificant threat to the loggerhead, Caretta caretta, in Florida. In addition the diseasehas been reported in olive ridley, Lepidochelys olivacea, populations as well. Datafrom several field studies suggests that high FP prevalence is associated with marineecosystems impacted by human activities, including agricultural, industrial, and urbandevelopment although the exact role of environmental co-factors is unclear. (18 page document)
    Description: Research Work Order no. 96
    Description: Final report.
    Keywords: Health ; Biology ; Green turtle ; Chelonia mydas ; fibropapillomatosis ; diseases ; Florida ; loggerhead turtle ; Caretta caretta ; marine turtle
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 6
    Electronic Resource
    Electronic Resource
    Development of monoclonal antibody for rock shrimp identification using enzyme-linked immunosorbent assay (1990)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 38 (1990), S. 2094-2100 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00101a019
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Starch Gel Electrophoretic Patterns of Murine Transferrin (1966)
    [s.l.] : Nature Publishing Group
    Nature 212 (1966), S. 1376-1377 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Investigations of murine transferrins by three different workers have shown that all strains examined possess the same transferrin type, with the exception of the CBA strain3'6'6. The most common type (b) has three bands which, in order of decreasing mobility, stain faintly, moderately and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2121376a0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Host-determined T cell fine specificity for self-H-2 in radiation bone-marrow chimeras of standard C57BL/6 (H-2b) mutantHz1 (H-2ba), and F1 mice (1978)
    Springer
    Immunogenetics 7 (1978), S. 73-77 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Reciprocal radiation bone-marrow chimeras were produced between the standard C57BL/6 (=B6) and the mutant B6.C-H-2 ba (=Hz1) strain. When infected with vaccinia virus, these chimeras, as well as an (Hz1 × B6)=→ Hz1 chimera, produced cytotoxic cells that killed vaccinia-infected H-2KkH-2Db target cells but failed to kill virus-infected H-2KbH-2Dd cells. Virus-infected (Hz1 × B6)F1 → B6 chimeras, however, killed both types of target. These experiments demonstrate strict T-cell specificity capable of differentiating between two molecules that apparently differ by a single amino acid substitution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01843990
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Further studies on the resistance of mice to myxoviruses (1966)
    Springer
    Archives of virology 19 (1966), S. 1-12 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mice from 16 inbred strains were challenged with neurotropic influenza A virus, strain WSA, in an effort to secure other examples of the occurrence of the genemx. All strains, with the exception of Z/Gw, were fully susceptible. Z/Gw mice showed some degree of resistance probably unrelated tomx. A2G mice were challenged with 12 different virus strains. All influenza viruses used (3 strains of fowl plague, MEL, FM-1, A2/Ann Arbor/2/60 and B/Ann Arbor/3/62) were definitely less virulent for A2G than for control (C3H, A, ICR) mice. Two strains of Newcastle disease virus, and one strain each of rabies, vesicular stomatitis and encephalomyocarditis viruses were of similar virulence for A2G and control mice. The possible bearing of these findings on the epidemiology of influenza is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01241985
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Melanoma cells growing in aggregates on a non-adhesive poly(HEMA) substrate exhibit polykaryocytosis but do not develop an increased metastatic capability (1984)
    Springer
    Clinical & experimental metastasis 2 (1984), S. 287-295 
    Details
    ISSN: 1573-7276
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract B16-F1 melanoma cells were plated onto plastic tissue-culture dishes rendered non-adhesive for cells by coating with 0–12 per cent poly(2-hydroxyethyl methylacrylate), poly(HEMA). These growth conditions caused the normally flat, adherent B16-F1 cells to grow as single cells in suspension. Within 24 hours, the rounded cells formed aggregates and grew at a slower rate than control cells grown at the same density on untreated plastic dishes. Microscopic observations provided evidence that polykaryocytosis was occurring among the aggregates. Following replating onto standard adhesive tissue-culture plastic, 20–30 per cent of the aggregates were observed to contain varying numbers of multinucleated giant cells (polykaryocytes). The study has revealed a previously undescribed propensity of certain B16-F1 cells cultivated as aggregates in suspension to develop into polykaryocytes, most probably as a result of spontaneous tumor cell-tumor cell fusion. The possible relevance of this behavior in vitro to events in tumor progression is discussed. This study, however, does not support the findings of others that the metastatic capability of B16-F1 cells is increased by such non-adherent culture conditions. No increase in metastatic potential was observed for B16-F1 cells, or for a low metastatic clone (Fl-7) derived from it, grown for 72 or 96 hours in a spherical configuration compared to control cells grown in a flat, adherent monolayer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00135168
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...