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  • 1
    Electronic Resource
    Electronic Resource
    Rapid induction of genomic demethylation and T-DNA gene expression in plant cells by 5-azacytosine derivatives (1989)
    Springer
    Plant molecular biology 12 (1989), S. 413-423 
    Details
    ISSN: 1573-5028
    Keywords: plant gene expression ; 5-methylcytosine ; 5-azacytidine ; A. tumefaciens ; T-DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have optimized conditions for demethylation of the genome and induction of a silent, hypermethylated T-DNA gene (ipt) by 5-azacytosine (5-azaCyt) derivatives in a suspension culture of tobacco cells. In this system, 5-azacytidine (5-azaC) is more effective in causing genomic demethylation and ipt gene induction than 5-azaCyt or 5-azadeoxycytidine (5-azadC). A single treatment with 2.5 μM 5-azaC resulted in a maximal level of ipt gene induction without inhibiting cell growth. However, we could not reduce the level of genomic methylation below approximately 2/3 of that found in untreated controls, even after extensive 5-azaC treatment. Furthermore, remethylation of the genome occurred after removal of 5-azaC. The use of 5-azaC as an inducer of silent plant genes is discussed, along with differences in the response of plant and animal genomes to demethylating agents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017581
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  • 2
    Electronic Resource
    Electronic Resource
    Allotetraploid origin ofAllium altyncolicum (Alliaceae, Allium sect.Schoenoprasum) as investigated by karyological and molecular markers (1997)
    Springer
    Plant systematics and evolution 206 (1997), S. 317-335 
    Details
    ISSN: 1615-6110
    Keywords: Alliaceae ; Allium altyncolicum ; A. ledebourianum ; A. schoenoprasum ; Allopolyploidy ; C-banding ; GISH ; ITS sequencing ; PCR-RFLP of cpDNA ; RFLP mapping of rDNA ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The tetraploidAllium altyncolicum (2n = 4x = 32) is considered to be of hybrid origin, because most of its morphological characters are intermediate between those of its putative parents,A. schoenoprasum andA. ledebourianum. In the present work an attempt has been made to ascertain its parentage by several methods: Giemsa C-banding, genomic in situ hybridization (GISH), PCR-RFLP of cpDNA, restriction enzyme mapping of the rDNA, and RAPDs. C-banding and GISH indicates clearly thatA. altyncolicum is a segmental allopolyploid.Allium schoenoprasum andA. ledebourianum are the most likely the parental species and the larger part of the genome ofA. altyncolicum (26 chromosomes) is derived fromA. schoenoprasum. The low genetic divergence between these three species was confirmed by the lack of sequence variation in the ITS sequences of nuclear rRNA genes and of the plastid rbcL-atpB intergenic spacer. Both parental species andA. altyncolicum could be distinguished by RFLP of the rDNA repeats. The geographic origin of the putative parental species was investigated using RAPDs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00987955
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  • 3
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression inEscherichia coli (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 355-361 
    Details
    ISSN: 1617-4623
    Keywords: Barley ; Light regulation ; NADPH-protochlorophyllide oxidoreductase ; cDNA sequence ; Transit peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA inEscherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02464904
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  • 4
    Electronic Resource
    Electronic Resource
    Chloroplast DNA restriction analysis and the infrageneric grouping ofAllium (Alliaceae) (1996)
    Springer
    Plant systematics and evolution 200 (1996), S. 253-261 
    Details
    ISSN: 1615-6110
    Keywords: Liliiflorae ; Alliaceae ; Allium ; Chloroplast DNA ; RFLP analysis ; phylogenetic relationships ; systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The utility of chloroplast DNA variation for checking a recently proposed infrageneric classification of the genusAllium was tested. cpDNA restriction patterns of 49 species representing the main subgenera, sections, and subsections of the existing classification were compared. 363 different fragments generated by 4 restriction enzymes were identified and analysed by UPGMA clustering. The resulting phenogram largely confirms the subgeneric classification based on an integration of morphological and other methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984939
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  • 5
    Electronic Resource
    Electronic Resource
    Origin of some minor vegetatively propagated Allium crops studied with RAPD and GISH (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 511-523 
    Details
    ISSN: 1573-5109
    Keywords: Allium ; crop evolution ; GISH ; hybridogenic cultivars ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three vegetative crops of sect. Cepa in genus Allium (Top onion, French grey shallot and viviparous triploid onion) of suspected hybridogenic origin were studied with genomic in situ hybridization (GISH) and random amplified polymorphic DNA (RAPD) markers. Related wild and cultivated species were included in the analysis in order to assess their contributions to the genomes of the investigated species. In A. × proliferum, the parental chromosomes derived from A. fistulosum and A. cepa were unequivocally identified by GISH, proving the hybrid status of this crop. The French grey shallot proved to belong to A. oschaninii according to the RAPD analysis and the GISH results, it is clearly separate from the normal shallots of A. cepa var. aggregatum. Thus the grey shallots are a new crop species and can be considered as an aggregatum form of the wild progenitor species A. oschaninii. The triploid viviparous onion comprises mostly A. cepa derived DNA in its genome. A non-cepa component could not be attributed to any of the sect. Cepa species included in this study and is most likely derived from one of the species of this section not yet identified by molecular means.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008647700251
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