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  • 1
    Electronic Resource
    Electronic Resource
    Gas exchange in intact isolated chloroplasts from Chlamydomonas reinhardtii during starch degradation in the dark (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 75 (1989), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: During starch degradation in intact isolated chloroplasts from Chlamydomonas reinhardtii gas exchange was studied with a mass spectrometer. Oxygen uptake by intact chloroplasts in the dark never exceeded 1.5% of the starch degradation rate [maximum 15 nmol O2 (mg Chl)−1 h−1 consumed. 1 000 nmol glucose (mg Chl)−1h−1 degraded]. Evolution of CO2 under aerobic conditions [9.8–28 nmol (mg Chl)−1 h−1] was stimulated by addition of 0.1–0.5 mM oxaloacetate [393–425 nmol CO2 (mg Chl)−1 h−1]. Pyridoxal phosphate (5 mM) inhibited starch degradation by more than 80%, but had no effect on O2 uptake. Starch degradation rates and CO2 evolution did not differ under acrobic and anaerobic conditions. Increasing Pi in the reaction medium from 0.5 mM to 5.0 mM stimulated starch degradation by 230 and 260% under aerobic and anaerobic conditions, respectively. A rapid autooxidation of reduced ferredoxin was observed in a reconstituted system consisting of purified Chlamydomonas ferredoxin, purified Chlamydomonas NADP-ferredoxin oxidoreductase (EC 1.6.7.1) and NADPH. Addition of isolated thylakoids from C. reinhardtii did not affect the rate of O2 uptake. Our results clearly indicate the absence of any oxygen requirement during starch degradation in isolated chloroplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1989.tb02071.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mechanisms of electrostimulated uptake of macromolecules into living cells (1990)
    Springer
    Naturwissenschaften 77 (1990), S. 543-545 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01139269
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Barium-cross-linked alginate beads: a simple, one-step method for successful immunoisolated transplantation of islets of Langerhans (1992)
    Springer
    Acta diabetologica 29 (1992), S. 99-106 
    Details
    ISSN: 1432-5233
    Keywords: Bioartificial pancreas ; Porcine islets ; Rat islets ; Xenogeneic transplantation ; Alginate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Transplantation of xenogeneic islets in immunoisolating membranes may solve the problems of the availability of human donor organs and long-term immunosuppression. Alginates are widely used for microencapsulation of isolated islets. This study presents data of a new method in which alginate was cross-linked with barium ions. In the perifusion experiment microencapsulated rat islets showed a biphasic insulin release with a short delay of the first phase. During static glucose challenge the insulin release ranged from 40% to 70% compared to free floating controls. In 3 of 11 diabetic mice, transplantation of 800 barium-alginate-bead encapsulated rat islets resulted in a non-fasting normoglycaemia at least up to 70 days. In the same model, transplantation of 3000 encapsulated porcine islets resulted in 6 of 10 recipients in normoglycaemia up to day 70, while 3 animals were still normoglycaemic on day 100. On day 21 after transplantation of encapsulated rat and porcine islets and intraperitoneal glucose tolerance test revealed rapid glucose assimilation in both groups. Histological examination demonstrated well-preserved islets at the end of the experiments. Immunohistological B-cell staining revealed the absence of recruitment of β-cells in the recipient's own pancreas. The barium-alginate microencapsulation method represents a simple one-step method for effective immunoisolated transplantation of large-scale islet preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572552
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  • 4
    Electronic Resource
    Electronic Resource
    Lymphoid activation by micro-and macroencapsulated islets during mixed lymphocyte islet culture (1993)
    Springer
    Acta diabetologica 30 (1993), S. 238-242 
    Details
    ISSN: 1432-5233
    Keywords: Alginate ; Islets of Langerhans ; Macroencapsulation ; Microencapsulation ; Transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have recently demonstrated long-lasting normoglycaemia after transplantation of barium alginate microencapsulated rat and porcine islets. Nevertheless the transplantation results obtained with different microencapsulation techniques have been controversial. Little is known about possible immune interactions between host and encapsulated islet. This study demonstrates in vitro stimulation of lymphoid cells by encapsulated islets that is similar to that of unencapsulated islets. This stimulation was reduced by a 4-day culture with unencapsulated islets only. After macroencapsulation of islets in hollow fibres a stimulatory effect was also observed, but this was less pronounced than after microencapsulation. Empty microcapsules as well as macrocapsules induced lymphoid proliferation as a result of mitogenic impurities in the encapsulation materials themselves. In the same donor-recipient combination in which we have shown successful transplantation, we found activation of the sensibilization arm of the immune system. This suggests that microencapsulation results in protection of the transplanted islets from the action of the effector arm. This lymphoid activation could be triggered by the mitogeniticity of the encapsulation material itself. In the case of alginates these mitogenic factors could not be abolished by culture (i.e. dialysis).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569935
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    Paper (German National Licenses)
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  • 5
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    Mixotrophic bioconversion with microalgae (2007)
    In:  EPIC3European Society of Micro Algal Biotechnology, 7th European Workshop, IGV Institut für Getreideverarbeitung GmbH, 11-13 June, Bergholz-Rehbrücke, Germany.
    Details
    Publication Date: 2019-07-17
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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