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  • 1
    Electronic Resource
    Electronic Resource
    An NMR investigation of the changes in plasma membrane triglyceride and phospholipid precursors during the activation of T-lymphocytes (1992)
    s.l. : American Chemical Society
    Biochemistry 31 (1992), S. 9098-9106 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00152a054
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Human retinoblastoma: in vitro differentiation and immunoglobulin superfamily antigen modulation by retinoic acid (1997)
    Springer
    Cancer immunology immunotherapy 44 (1997), S. 189-196 
    Details
    ISSN: 1432-0851
    Keywords: Key words Differentiation ; Immunoglobulin superfamily antigens ; Y79 retinoblastoma cell line ; All-trans retinoic acid ; Retina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Suspension and attachment cultures of Y79 human retinoblastoma cells were treated with all-trans retinoic acid (RA) for up to 10 days to assess its effect on growth and cell-surface expression of immunoglobulin superfamily antigens MHC class I and class II, ICAM-1, NCAM and Thy1. RA up to 10 μM induced growth inhibition, and marked morphological differentiation with extension of prominent processes resembling neurites was seen in attachment cultures. However, above 10 μM RA produced extensive cell death. We also observed increased cell-surface expression of MHC class I, ICAM-1, NCAM and Thy1 on Y79 cells treated with 10 μM over 10 days; constitutive MHC class II expression was not apparent, nor did RA treatment appear to induce Y79 cells to express MHC class immunoreactivity. The up-modulation of cell-adhesion molecules (NCAM, ICAM-1 and Thy1) and immune recognition molecules (NCAM, ICAM-1 and MHC class I), associated with reduced growth and tumour cell differentiation, suggests that RA may have a potential role in regulating the growth and development of retinoblastoma tumours.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050372
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  • 3
    Electronic Resource
    Electronic Resource
    The appearance of surface H-2 antigens on fetal and postnatal murine hepatocytes in vivo and in vitro: A comparative study of MHC control (1986)
    Springer
    Immunogenetics 23 (1986), S. 18-23 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have collected data on the kinetics of in vivo development of H-2b antigens in fetal and postnatal hepatocytes from days 15–89 postcoitum (pc) in C57BL/10 mice using a sensitive immunoferritin labeling method combined with electron microscopy. We compared these data with data on the kinetic development of H-2b antigens on fetal hepatocytes cultured from days 15, 16, and 17 pc onwards. Using the same techniques, we also compared the surface concentrations of H-2 antigens on the hepatocytes of pregnant and age-matched male and virgin female mice at day 110 pc. We found that the surface concentration of H-2 antigens on fetal and postnatal hepatocytes increased with time but that the birth event was associated with an increase in H-2 antigen expression from 60–80 ferritin grains per meter (F/m) to 190 F/m followed by a decrease to 115 F/m within 72 h of birth. The concentration of antigens on postnatal hepatocytes increased gradually and reached a maximum of 640 F/m on day 41 pc. By day 89 pc, however, this level had decreased 433 F/m, which was not significantly different from that found in day 110 pc males and virgin females. In contrast to previous findings, we found that hepatocytes cultured from days 15, 16, and 17 pc exhibited a large increase in H-2 surface antigen concentration within 48 h from 60–80 F/m to 1500–2200 F/m. After day 2, the H-2 concentration decreased and by days 14–17 in culture it was not significantly different from day 110 pc male and virgin female levels (485 F/m). Lastly, we found that the H-2 concentration on hepatocytes from pregnant mice was increased significantly (725 F/m) compared with the concentration of hepatocytes from day 110 pc males or virgin females. We postulate that the control of cell-surface major histocompatibility complex antigen concentration is achieved by a combination of intra and extracellular mechanisms and we discuss how this might be of benefit to the animal in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376517
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  • 4
    Electronic Resource
    Electronic Resource
    Analysis of cell cycle activity and population dynamics in heterogeneous plant cell suspensions using flow cytometry (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 515-528 
    Details
    ISSN: 0006-3592
    Keywords: flow cytometry ; plant cell culture ; bromodeoxyuridine ; cell cycle ; hydrodynamic shear ; temperature effects ; Solanum aviculare ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Flow cytometry was used to measure cell cycle parameters in Solanum aviculare plant cell suspensions. Methods for bromodeoxyuridine (BrdU) labeling of plant nuclei were developed so that cell cycle times and the proportion of cells participating in growth could be determined as a function of culture time and conditions. The percentage of cells active in the cell cycle at 25°C decreased from 52% to 19% within 7.6 d of culture; presence of a relatively large proportion of non-active cells was reflected in the results for culture growth. While the maximum specific growth rate of the suspensions at 25°C was 0.34 d-1 (doubling time: 2.0 d), the specific growth rate of active cells was significantly greater at 0.67 d-1, corresponding to a cell cycle time of 1.0 d. A simple model of culture growth based on exponential and linear growth kinetics and the assumption of constant cell cycle time was found to predict with reasonable accuracy the proportion of active cells in the population as a function of time. Reducing the temperature to 17°C lowered the culture growth rate but prolonged the exponential growth phase compared with 25°C; the percentage of cells participating in the cell cycle was also higher. Exposure of plant cells to different agitation intensities in shake flasks had a pronounced effect on the distribution of cells within the cell cycle. The proportion of cells in S phase was 1.8 times higher at a shaker speed of 160 rpm than at 100 rpm, while the frequency of G0 + G1 cells decreased by up to 27%. Because of the significant levels of intraculture heterogeneity in suspended plant cell systems, flow cytometry is of particular value in characterizing culture properties and behavior. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58: 515-528, 1998.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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