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  • 1
    Publication Date: 2024-02-06
    Description: The author wants to highlight the important role of mesopelagic fish in the conservation of marine living resources. Therefore, in order to further clarify and focus research point on the conservation of marine living resources, the authors replaced the MPA with the protected areas, and slightly modified several other vague places. In these corrections, most of them are the replacement of the terms. The original article has been corrected. The text “marine protected areas” is replaced with “conservation areas for marine living resources” in the title, Line 2–3. The text "the Marine protected areas" is replaced with "mesopelagic fish" in Line 24. The text "the MPA with protected areas" is replaced in Line 10, Line 23, Line 359, Line 370, Line 508, Line 519, Line 546, Line 547, Line 549, Line 552, Line 554, Line 556, Line 558, Line 562, Line 566, Line 769, Several vague places using protected areas are slightly modified in Line 15, Line 20, Line 21, Line 22, Line 155–156, Line 331–332, Line 356–357, Line 359, Line 361, Line 363, Line 365–366, Line 504, Line 505, Line 506, Line 513, Line 783–784. For replacement of the MPA using protected areas, the Fig. 7 and legend are modified without the change of the numbers. (Figure presented.) The text “marine protected areas” is replaced with “conservation areas for marine living resources” in the title in Supplementary information.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
    Format: application/pdf
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  • 2
    Publication Date: 2024-04-24
    Description: Mesopelagic fish (meso-fish) are central species within the Southern Ocean (SO). However, their ecosystem role and adaptive capacity to climate change are rarely integrated into marine protected area (MPAs) assessments. This is a pity given their importance as crucial prey and predators in food webs, coupled with the impacts of climate change. Here, we estimate the habitat distribution of nine meso-fish using an ensemble model approach (MAXENT, random forest, and boosted regression tree). Four climate model simulations were used to project their distribution under two representative concentration pathways (RCP4.5 and RCP8.5) for short-term (2006–2055) and long-term (2050–2099) periods. In addition, we assess the ecological representativeness of established and proposed MPAs under climate change scenarios using meso-fish as indicator species. Our models show that all species shift poleward in the future. Lanternfishes (family Myctophidae) are predicted to migrate poleward more than other families (Paralepididae, Nototheniidae, Bathylagidae, and Gonostomatidae). In comparison, lanternfishes were projected to increase habitat area in the eastern SO but lose area in the western SO; the opposite was projected for species in other families. Important areas (IAs) of meso-fish are mainly distributed near the Antarctic Peninsula and East Antarctica. Proposed MPAs cover 23% of IAs at present and 38% of IAs in the future (RCP8.5, long-term future). Many IAs of meso-fish still need to be included in MPA proposals, such as the Prydz Bay and the seas around the Antarctic Peninsula. Our results provide a framework for designing new MPAs incorporating climate change adaptation strategies for MPA management.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
    Format: application/pdf
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Munksgaard International Publishers
    Physiologia plantarum 125 (2005), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Xyloglucan endotransglucosylase/hydrolases (XTHs) are thought to be involved in various aspects of plant development by modifying the structure of xyloglucan cross-links. To address one of the roles of XTHs in plant growth, we identified an XTH, VrXTH1, in the mungbean through a differential reverse transcriptase-polymerase chain reaction. The deduced amino acid sequence of VrXTH1 shows high similarity to other XTHs. In addition, a signal peptide consisting of 17 amino acids is found at the N-terminus. The gene expression of VrXTH1 was differentially regulated in tissues and was higher in hypocotyls and stems than it was in other tissues. The steady state level of VrXTH1 transcripts was closely related to the elongation regions of hypocotyls. Notably, in the elongation region of hypocotyls, most VrXTH1 mRNAs were limited to the epidermis and to some layers of the cortex that act as growth-limiting tissue. Growth-promoting hormones, such as auxin and brassinolide, strongly enhanced mRNA accumulation of VrXTH1. However, abscisic acid, a hormone which is antagonistic to auxin, acted as a downregulator. Overall, VrXTH1 seems to play a role in plant growth at the gene level and, thus, by possibly altering cell wall morphogenesis in mungbean hypocotyls.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The breaking of immune tolerance against autologous angiogenic endothelial cells should be a useful approach for cancer therapy. Here we show that immunotherapy of tumors using fixed xenogeneic whole endothelial cells as a vaccine was effective in affording protection from tumor growth, inducing ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 104 (1972), S. 275-281 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Removing endogenous ethylene by hypobaric treatment, or displacing it with carbon dioxide inhibits hook development in etiolated pea seedlings. When seedlings are returned to a normal atmosphere, hook formation occurs in darkness. Addition of ethylene accelerates this process. When ethylene induces hook formation, cell division in the hook tissue is rather inhibited by the gas. These data suggest that endogenous ethylene causes formation of the hook by inducing expansion of certain cells.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Planta 110 (1973), S. 227-235 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Synthesis of anthocyanin pigments in etiolated cabbage seedlings is influenced by ethylene at concentrations higher than 10 ppb, and etiolated seedlings produce sufficient ethylene to influence their anthocyanin synthesis. When escape of endogenous ethylene from this tissue is enhanced by means of hypobaric treatment, anthocyanin synthesis is accelerated. Stimulation of anthocyanin synthesis by brief red illumination is completely prevented by applied ethylene and indoleacetic acid inhibits anthocyanin synthesis by stimulating ethylene production. Red light reduces endogenous as well as auxin-induced ethylene production and there is a close correlation between light-induced inhibition of ethylene synthesis and stimulation of anthocyanin formation. We suggest that in part photo-induced anthocyanin synthesis is due to a lowered ethylene content in light-treated tissue.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5028
    Keywords: hypocotyl ; mung bean ; radicle ; telomere ; telomere-binding protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have identified and characterized protein factors from mung bean (Vigna radiata) nuclear extracts that specifically bind the single-stranded G-rich telomeric DNA repeats. Nuclear extracts were prepared from three different types of plant tissue, radicle, hypocotyl, and root, in order to examine changes in the expression patterns of telomere-binding proteins during the development of mung bean. At least three types of specific complexes (A, B, and C) were detected by gel retardation assays with synthetic telomere and nuclear extract from radicle tissue, whereas the two major faster-migrating complexes (A and B) were formed with nuclear extracts from hypocotyl and root tissues. Gel retardation assays also revealed differences in relative amount of each complex forming activity in radicle, hypocotyl, and root nuclear extracts. These data suggest that the expression of telomere-binding proteins is developmentally regulated in plants, and that the factor involved in the formation of complex C may be required during the early stages of development. The binding factors have properties of proteins and are hence designated as mung bean G-rich telomere-binding proteins (MGBP). MGBPs bind DNA substrates with three or more single-stranded TTTAGGG repeats, while none of them show binding affinity to either double-stranded or single-stranded C-rich telomeric DNA. These proteins have a lower affinity to human telomeric sequences than to plant telomeric sequences and do not exhibit a significant binding activity to Tetrahymena telomeric sequence or mutated plant telomeric sequences, indicating that their binding activities are specific to plant telomere. Furthermore, RNase treatment of the nuclear extracts did not affect the complex formation activities. This result indicates that the single-stranded telomere-binding activities may be attributed to a simple protein but not a ribonucleoprotein. The ability of MGBPs to bind specifically the single-stranded TTTAGGG repeats may suggest their in vivo functions in the chromosome ends of plants.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5028
    Keywords: 1-aminocyclopropane-1-carboxylate synthase ; auxin ; brassinosteroid ; ethylene ; gene expression ; hypocotyl ; promoter ; transgenic tobacco ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Indole-3-acetic acid (IAA) markedly increased ethylene production by inducing the expression of three 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNAs (pVR-ACS1, pVR-ACS6 and pVR-ACS7) in mung bean hypocotyls. Results from nuclear run-on transcription assay and RNA gel blot studies revealed that all three genes were transcriptionally active displaying unique patterns of induction by IAA and various hormones in etiolated hypocotyls. Particularly, 24-epibrassinolide (BR), an active brassinosteroid, specifically enhanced the expression of VR-ACS7 by a distinct temporal induction mechanism compared to that of IAA. In addition, BR synergistically increased the IAA-induced VR-ACS6 and VR-ACS7 transcript levels, while it effectively abolished both the IAA- and kinetin-induced accumulation of VR-ACS1 mRNA. In light-grown plants, VR-ACS1 was induced by IAA in roots, and VR-ACS6 in epicotyls. IAA- and BR-treatments were not able to increase the VR-ACS7 transcript in the light-grown tissues. These results indicate that the expression of ACC synthase multigene family is regulated by complex hormonal and developmental networks in a gene- and tissue-specific manner in mung bean plants. The VR-ACS7 gene was isolated, and chimeric fusion between the 2.4 kb 5′-upstream region and the β-glucuronidase (GUS) reporter gene was constructed and introduced into Nicotiana tabacum. Analysis of transgenic tobacco plants revealed the VR-ACS7 promoter-driven GUS activity at a highly localized region of the hypocotyl-root junction of control seedlings, while a marked induction of GUS activity was detected only in the hypocotyl region of the IAA-treated transgenic seedlings where rapid cell elongation occurs. Although there was a modest synergistic effect of BR on the IAA-induced GUS activity, BR alone failed to increase the GUS activity, suggesting that induction of VR-ACS7 occurs via separate signaling pathways in response to IAA and BR. A scheme of the multiple regulatory pathways for the expression of ACC synthase multigene family by auxin and BR is presented.
    Type of Medium: Electronic Resource
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  • 9
    Publication Date: 2012-09-25
    Description: We previously showed that antigen immunization in the presence of the immunosuppressant dexamethasone (a strategy we termed “suppressed immunization”) could tolerize established recall responses of T cells. However, the mechanism by which dexamethasone acts as a tolerogenic adjuvant has remained unclear. In the present study, we show that dexamethasone enriches CD11c lo CD40 lo macrophages in a dose-dependent manner in the spleen and peripheral lymph nodes of mice by depleting all other CD11c + CD40 + cells including dendritic cells. The enriched macrophages display a distinct MHC class II (MHC II) lo CD86 hi phenotype. Upon activation by antigen in vivo, CD11c lo CD40 lo macrophages upregulate IL-10, a classic marker for tolerogenic antigen-presenting cells, and elicit a serum IL-10 response. When presenting antigen in vivo, these cells do not elicit recall responses from memory T cells, but rather stimulate the expansion of antigen-specific regulatory T cells. Moreover, the depletion of CD11c lo CD40 lo macrophages during suppressed immunization diminishes the tolerogenic efficacy of the treatment. These results indicate that dexamethasone acts as a tolerogenic adjuvant partly by enriching the CD11c lo CD40 lo tolerogenic macrophages.
    Print ISSN: 0014-2980
    Electronic ISSN: 1521-4141
    Topics: Medicine
    Published by Wiley-Blackwell
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  • 10
    Publication Date: 2013-07-12
    Description: To quantify more precisely and more reliably diffusion and reaction properties of biomolecules in living cells, a novel closed description in 3D of both the bleach and the post-bleach segment of fluorescence recovery after photobleaching (FRAP) data acquired at a point, i.e., a diffraction-limited observation area, termed point FRAP, is presented. It covers a complete coupled reaction–diffusion scheme for mobile molecules undergoing transient or long-term immobilization because of binding. We assess and confirm the feasibility with numerical solutions of the differential equations. By applying this model to free EYFP expressed in HeLa cells using a customized confocal laser scanning microscope that integrates point FRAP and fluorescence correlation spectroscopy (FCS), the applicability is validated by comparison with results from FCS. We show that by taking diffusion during bleaching into consideration and/or by employing a global analysis of series of bleach times, the results can be improved significantly. As the point FRAP approach allows to obtain data with diffraction-limited positioning accuracy, diffusion and binding properties of the exon–exon junction complex (EJC) components REF2-II and Magoh are obtained at different localizations in the nucleus of MCF7 cells and refine our view on the position-dependent association of the EJC factors with a maturating mRNP complex. Our findings corroborate the concept of combining point FRAP and FCS for a better understanding of the underlying diffusion and binding processes. © 2013 International Society for Advancement of Cytometry
    Electronic ISSN: 1552-4930
    Topics: Biology , Medicine
    Published by Wiley-Blackwell
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