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  • 1
    ISSN: 1573-868X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Barotolerant bacterium was isolated from sediment sample which was obtained from the depth of 4033 m in the Izu-Ogasawara Trench. The physiological property, growth characteristics and fatty acid composition were examined. The strain was a psychrotrophic and barotolerant bacterium, and was identified as species in the genusAlteromonas. The fatty acids of the strain were from C12 to C18. As the growth pressure increased, the portion of unsaturated fatty acid in membrane fraction increased due to an increase in the portion of C17∶1 and C18∶1, while the relative portion of C16∶0 and C16∶1 decreased. On the other hand, as the growth temperature decreased, the portion of unsaturated fatty acid increased due to the increase in the portion of C16∶1 and C18∶1.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We isolated highly intact nuclei from cells of a haploid strain ofSaccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 6 (1992), S. 357-362 
    ISSN: 0268-2605
    Keywords: Arsenic ; β-carotene ; carbohydrate ; Dunaliella salina ; microalgae ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The unicellular marine alga, Dunaliella salina 19/30 was grown in seawater containing an inorganic arsenic concentration (Na2HAsO4) up to 2000 mg dm-3. The cells survived even at 5000 mg dm-3. The arsenic concentration of the cells increased with an increase of the surrounding arsenic concentration. Arsenic in D. salina was also greatly affected by addition of phosphorus. The arsenic-tolerance behavior of D. salina seemed to suggest that the algae have a function to prevent accumulation of inorganic arsenic by increasing the β-carotene, fatty-acid (C18:1, C18:3) and water-extractable carbohydrate content in the cells. Arsenic accumulation also rose steadily with an increase in the nitrogen concentration in the medium.
    Additional Material: 3 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 4 (1990), S. 261-264 
    ISSN: 0268-2605
    Keywords: Arsenic ; selenium ; bioaccumulation ; Dunaliella sp. ; microalgae ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The unicellular marine alga, Dunaliella sp., was grown in seawater containing selenium and arsenic. Dunaliella sp. readily took up selenite from the medium over a broad range of concentrations (10-10-10-5mol dm-3). In comparison, uptake from selenate was generally minimal. Selenite was detectable in cells within 24 h and concentrations increased gradually with time. Also iron and zinc concentrations in Dunaliella sp. cells were largely affected by addition of arsenic and gallium. Arsenic concentrations in cultures were largely correlated to the accumulation of arsenic by Dunaliella sp., but did not affect the accumulation of selenium by Dunaliella sp. This implied that the accumulation process of selenium was different from that of arsenic.
    Additional Material: 3 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 10 (1996), S. 753-756 
    ISSN: 0268-2605
    Keywords: Dunaliella sp ; arsenate ; arsenite ; reduction ; water-soluble fraction ; gel filtration ; Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Uptake and reduction of arsenate [AS(V)] by Dunaliella sp. cells were determined to investigate the metabolic processes of arsenic in the alga. Cellular uptake of arsenic by Dunaliella sp. cells was markedly affected by the form of arsenic in the medium. The content of arsenic taken up by Dunaliella sp. cells increased rapidly with time on addition of As(V) to the medium. However, in the case of addition of arsenite [As(III)], the gradient of arsenic uptake by Dunaliella sp. cells was low, and arsenic content was small. In the water-soluble fraction of arsenic taken up by Dunaliella sp. cells with exposure to As(V), arsenic was in the forms of organic arsenic, As(V) and As(III). The content of As(V) in the water-soluble fraction increased with exposure time. The content of As(III) also increased with time, but remained constant after 5 h of exposure. On the other hand, organic arsenic content was small and did not increase with time. It was found that Dunaliella sp. takes up As(V) and readily reduces it to As(III)
    Additional Material: 3 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 10 (1996), S. 721-726 
    ISSN: 0268-2605
    Keywords: arsenic ; accumulation ; Chattonella antiqua ; microalgae ; Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Rhaphydophyceae Chattonella antiqua (Hada) Ono was grown in seawater containing an arsenic concentration up to 50 mg dm-3, and survived even at 200 mg dm-3. The arsenic content increased with an increase of the surrounding arsenic, iron and manganese concentrations. However, arsenic accumulation was unaffected by phosphorus concentration. Also, arsenic content in C. antiqua decreased at a selenium concentration of up 20 mg dm-3, and was reduced by the addition of antimony. In the living cells, about 52% of the arsenic which accumulated in each cell was found in the intracellular fraction, 27% in the lipid fraction, and 21% in the cell wall fraction.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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