ISSN:
1432-072X
Keywords:
Key words Pterin derivatives
;
Wolinella succinogenes
;
Polysulfide reductase
;
Formate dehydrogenase
;
Molybdopterin guanine dinucleotide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Pterin derivatives were extracted from formate dehydrogenase and from polysulfide reductase of Wolinella succinogenes and converted to 6-carboxypterin. The amounts of 6-carboxypterin were consisted with the molybdenum content of the enzymes. The bis(carboxamidomethyl) derivatives of the cofactors showed absorption spectra that were identical with that of the corresponding molybdopterin guanine dinucleotide derivative (cam MGD). After hydrolysis of the derivatives with nucleotide pyrophosphatase in the presence of alkaline phosphatase, guanosine was formed together with a compound showing the properties of dephospho-bis(carboxamidomethyl)-molybdopterin. It is conluded that both formate dehydrogenase and polysulfide reductase of W. succinogenes contain molybdopterin guanine dinucleotide.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00301844
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