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  • 1
    Publication Date: 2022-05-26
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 6 (2016): 22541, doi:10.1038/srep22541.
    Description: The rock-hosted, oceanic crustal aquifer is one of the largest ecosystems on Earth, yet little is known about its indigenous microorganisms. Here we provide the first phylogenetic and functional description of an active microbial community residing in the cold oxic crustal aquifer. Using subseafloor observatories, we recovered crustal fluids and found that the geochemical composition is similar to bottom seawater, as are cell abundances. However, based on relative abundances and functional potential of key bacterial groups, the crustal fluid microbial community is heterogeneous and markedly distinct from seawater. Potential rates of autotrophy and heterotrophy in the crust exceeded those of seawater, especially at elevated temperatures (25°C) and deeper in the crust. Together, these results reveal an active, distinct, and diverse bacterial community engaged in both heterotrophy and autotrophy in the oxygenated crustal aquifer, providing key insight into the role of microbial communities in the ubiquitous cold dark subseafloor biosphere. An Author Correction to this article was published on 16 April 2020
    Description: This work was supported by NSF OCE1062006 to JAH, NSF OCE1061934 to PRG, and NSF OCE1061827 to BTG. The Center for Dark Energy Biosphere Investigations (C-DEBI) (OCE-0939564), a National Science Foundation-funded Science and Technology Centers of Excellence also supported the participation of CGW, THL, JPC, JAH, BT, and CHH, as well as JLM and UJ through C-DEBI Postdoctoral Fellowships.
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2022-06-13
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Chen, S.-C., Ji, J., Popp, D., Jaekel, U., Richnow, H.-H., Sievert, S. M., & Musat, F. Genome and proteome analyses show the gaseous alkane degrader Desulfosarcina sp. strain BuS5 as an extreme metabolic specialist. Environmental Microbiology, 24, (2022): 1964-1976, https://doi.org/10.1111/1462-2920.15956.
    Description: The metabolic potential of the sulfate-reducing bacterium Desulfosarcina sp. strain BuS5, currently the only pure culture able to oxidize the volatile alkanes propane and butane without oxygen, was investigated via genomics, proteomics and physiology assays. Complete genome sequencing revealed that strain BuS5 encodes a single alkyl-succinate synthase, an enzyme which apparently initiates oxidation of both propane and butane. The formed alkyl-succinates are oxidized to CO2 via beta oxidation and the oxidative Wood–Ljungdahl pathways as shown by proteogenomics analyses. Strain BuS5 conserves energy via the canonical sulfate reduction pathway and electron bifurcation. An ability to utilize long-chain fatty acids, mannose and oligopeptides, suggested by automated annotation pipelines, was not supported by physiology assays and in-depth analyses of the corresponding genetic systems. Consistently, comparative genomics revealed a streamlined BuS5 genome with a remarkable paucity of catabolic modules. These results establish strain BuS5 as an exceptional metabolic specialist, able to grow only with propane and butane, for which we propose the name Desulfosarcina aeriophaga BuS5. This highly restrictive lifestyle, most likely the result of habitat-driven evolutionary gene loss, may provide D. aeriophaga BuS5 a competitive edge in sediments impacted by natural gas seeps.
    Description: This study was financed by the Max Planck Society and by the Helmholtz Association of German Research Centres. The draft genome was sequenced as part of the U.S. Department of Energy Joint Genome Institute (DOE-JGI) Community Science Program project 1078203 awarded to S. M. Sievert and F. Musat. The work conducted by the DOE-JGI, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. Lynne Goodwin (Los Alamos National Laboratory) is acknowledged for project management support of the draft genome sequencing. Further support was provided by the U.S. National Science Foundation grant MCB-0702677 (to SMS), and by the Helmholtz Association grant ERC-RA-0020 (to FM). We acknowledge the Centre for Chemical Microscopy (ProVIS) platform at the Helmholtz Centre for Environmental Research – UFZ, for using their analytical facilities. ProVIS is supported by European Regional Development Funds (EFRE – Europe funds Saxony). We acknowledge the Bundesministerium für Bildung und Forschung (BMBF)-funded German Network for Bioinformatics Infrastructure de.NBI (031A537B, 031A533A, 031A538A, 031A533B, 031A535A, 031A537C, 031A534A, 031A532B) for providing computational resources.
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Location Call Number Limitation Availability
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