ISSN:
1618-2650
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Capillary zone electrophoresis (CZE) has been applied for the separation and quantification of the polyamines putrescine (PUT), cadaverine (CAD), spermidine (SPD) and spermine (SPM), after their derivatization with the fluoresceinisothiocyanate isomer I (FITC). The derivatization conditions (character of the derivatization medium and concentration, organic modifier, pH and temperature) and CZE operation conditions have been optimized with respect to a sensitive Laser induced fluorescence detection (λexcitation=488 nm/λdetection=520 nm). A complete separation of CAD from PUT was impossible under the conditions chosen. The detection limits and relative standard deviations for SPM, SPD, CAD, PUT were determined to be 2.9 (12%), 3.2 (7.6%), 0.7 (4.6%), 1.2 (7.6%) nmol/L, respectively. The method has been applied to a pine needle extract.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00323628
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