Keywords:
Cytology.
;
Neurons.
;
Electronic books.
Description / Table of Contents:
This book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. * Contains culture methodology for more than a dozen types of CNS and PNS neurons * Includes most recent and reliable techniques from expert practitioners for specific experimental applications * Addresses the latest strategies for transfecting neurons.
Type of Medium:
Online Resource
Pages:
1 online resource (459 pages)
Edition:
1st ed.
ISBN:
9780080496634
Series Statement:
Issn Series ; v.Volume 71
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=296959
DDC:
571.6
Language:
English
Note:
Cover -- Contents -- Contributors -- Preface -- Chapter 1. Comparing the Properties of Neuronal Culture Systems: A Shopping Guide for the Cell Biologist -- I. Introduction -- II. Approaching Experimental Questions Using Neurons -- III. Choosing a Neuronal Culture System -- IV. Conclusions -- References -- Chapter 2. Growing and Working with Peripheral Neurons -- I. Introduction -- II. Methods -- III. Cultures -- References -- Chapter 3. Dissection and Culturing of Chick Ciliary Ganglion Neurons: A System Well Suited to Synaptic Study -- I. General Introduction -- II. Materials -- III. Methods -- IV. Variations on Dissection, Dissociation, and Culturing Themes -- V. Concluding Comments -- References -- Chapter 4. The Culture of Chick Forebrain Neurons -- I. Introduction -- II. Growth and Development Characteristics -- III. Isolation of Single Chick Forebrain Neurons -- IV. Culture Conditions for Chick Forebrain Neurons -- References -- Chapter 5. Growing and Working with Spinal Motor Neurons -- I. Introduction -- II. Incubation of Fertilized Chicken Eggs -- III. Preparation of Chick Embryo -- IV. Dissection of Intact Spinal Cords and Isolation of Ventral Halves -- V. Enzymatic and Mechanical Dissociation of Intact Spinal Cords or Ventral Halves -- VI. Plating Motor Neurons Spinal Cord Neurons -- VII. Motor Neuron Enrichment by Density Gradient Centrifugation -- VIII. Experimental Use of Motor Neuron Cultures and Spinal Cord Cultures -- IX. Preparation of Solutions, Culture Dishes, Media, and Media Supplements -- References -- Chapter 6. Avian Purkinje Neuronal Cultures: Extrinsic Control of Morphology by Cell Type and Glutamate -- I. Introduction -- II. Methods and Systems -- III. Applications -- References -- Chapter 7. Culturing Hippocampal and Cortical Neurons -- I. Introduction -- II. Acquisition of Hippocampal and Cortical Neurons.
,
III. Short-Term Culture Methods -- IV. Long-Term Culture Methods -- V. Summary -- References -- Chapter 8. Working with Xenopus Spinal Neurons in Live Cell Culture -- I. Introduction -- II. Neuronal Labeling -- III. Culturing Xenopus Spinal Neurons -- IV. Live Cell Imaging and Manipulations -- V. Summary -- References -- Chapter 9. Culturing Neurons from the Snail Helisoma -- I. Introduction -- II. Maintaining Animals -- III. Initial Dissection -- IV. Culturing Nerve Cells -- References -- Chapter 10. The Tibial-1 Pioneer Pathway. An in Vivo Model for Neuronal Outgrowth and Guidance -- I. Introduction -- II. Development of the Tibial-1 (Ti1) Pathway -- III. Guidance of Ti1 Axons -- IV. Analysis of Axon Guidance Mechanisms in Grasshopper Procedures -- V. Laboratory Protocols -- References -- Chapter 11. Techniques to Dissect Cellular and Subcellular Function in the Drosophila Nervous System -- I. Introduction -- II. Background Sources of Information -- III. Drosophila as a Genetic Model System for Molecular Neurobiology -- IV. Biochemistry of the Drosophila Nervous System -- V. Cell Biology Techniques in the Drosophila Nervous System -- VI. Functional Analysis Techniques in the Drosophila Nervous System -- VII. Drosophila Electrophysiology -- References -- Chapter 12. PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells -- I. Introduction -- II. Propagation and Culture of PC12 Cells -- III. Studying Secretion Using PC12 Cells -- IV. Immunocytochemical Detection of Proteins in Intact and Permeable PC12 Cells -- References -- Chapter 13. B35 Neuroblastoma Cells: An Easily Transfected, Cultured Cell Model of Central Nervous System Neurons -- I. Introduction -- II. Applications Utilizing B35 and B50 Cells -- III. Protocols -- References -- Chapter 14. Live-Cell Imaging of Slow Axonal Transport in Cultured Neurons.
,
I. Introduction -- II. Culturing Neurons from Superior Cervical Ganglia -- III. Transfecting Neurons by Nuclear Injection -- IV. Injecting Neurons with Fluorescent Proteins -- V. Observing Movement -- VI. Conclusion -- References -- Chapter 15. Making Proteins into Drugs: Assisted Delivery of Proteins and Peptides into Living Neurons -- I. Introduction -- II. Principles -- III. Materials -- IV. Methods -- V. Conclusions and Perspectives -- References -- Chapter 16. Tranfection of Primary Central and Peripheral Nervous System Neurons by Electroporation -- I. Introduction -- II. Electroporation Theory and Principles -- III. Electroporation Protocol for Chick Primary Neurons -- IV. Rationale for Setting Electroporation Parameters -- V. Discussion -- References -- Chapter 17. Biolistic Transfection -- I. Introduction -- II. Parameters that Affect Success of the Method -- III. Factors that May Affect Performance but Have Not Been Tested -- IV. Future Developments or Improvements -- V. Conclusion -- References -- Chapter 18. Expression of Transgenes in Primary Neurons from Chick Peripheral and Central Nervous Systems by Retroviral Infection of Early Embryos -- I. Introduction -- II. Building Retroviral Vectors and Growing Functional Virus -- III. Infecting Early Chick Embryos by Injection -- IV. Dissecting Embryos and Culturing Neurons -- V. Conclusion -- References -- Chapter 19. Production and Use of Replication-Deficient Adenovirus for Transgene Expression in Neurons -- I. Introduction -- II. Preparing Adenoviral Expression Vectors -- III. Production of Adenovirus in HEK293 Cells -- IV. Purification and Storage of Adenovirus Stocks -- V. Titer Assays -- VI. Safety Issues -- VII. Infection of Primary Neurons -- VIII. Conclusions and Perspectives -- References -- Index -- Volume in Series.
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