Keywords:
High performance liquid chromatography.
;
Electronic books.
Description / Table of Contents:
This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented.
Type of Medium:
Online Resource
Pages:
1 online resource (961 pages)
Edition:
1st ed.
ISBN:
9781351440745
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=5226980
DDC:
574.19245
Language:
English
Note:
Cover -- Title Page -- Copyright Page -- Preface -- Table of Contents -- SECTION I: PRACTICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY -- Properties of Peptides/Proteins and Practical Implications -- Standard Chromatographic Conditions for Size-Exclusion, Ion-Exchange, Reversed-Phase and Hydrophobic Interaction Chromatography -- Preventive Maintenance and Troubleshooting LC Instrumentation -- Tools and Techniques to Extend LC Column Lifetimes -- Mobile Phase Preparation and Column Maintenance -- HPLC Guard Columns -- On-Line Derivatization of Silica Supports for Regeneration of Reversed-Phase Columns -- SECTION II: THEORETICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY -- HPLC Terminology: Practical and Theoretical -- High-Performance Liquid Chromatography of Peptides and Proteins: General Principles and Basic Theory -- High-Performance Liquid Chromatography of Peptides and Proteins: Quantitative Relationships for Isocratic and Gradient Elution of Peptides and Proteins -- SECTION III: SIZE-EXCLUSION CHROMATOGRAPHY -- Requirement for Peptide Standards to Monitor Ideal and Non-Ideal Behavior in Size- Exclusion Chromatography -- High-Perfonnance Size-Exclusion Chromatography of Proteins -- Size-Exclusion Chromatography of Proteins -- Size-Exclusion High-Performance Liquid Chromatography of Integral Membrane Proteins and the Effect of Detergents on Immunological Activity -- A Reversed-Phase Trace Enrichment Technique for the Loading of Size-Exclusion HPLC Columns -- SECTION IV: ION-EXCHANGE CHROMATOGRAPHY -- The Use of Peptide Standards for Monitoring Ideal and Non-Ideal Behavior in Cation-Exchange Chromatography -- Ion-Exchange High-Performance Liquid Chromatography of Peptides -- Use of Ion-Exchange Cartridges for Batch Extraction in the Purification of Pituitary Peptides -- High-Performance Ion-Exchange Chromatography of Proteins.
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High-Performance Ion-Exchange Chromatography of Proteins: Application to the Purification of Enzymes -- Anion Exchange High-Performance Liquid Chromatography of Viral Proteins -- Sample Preparation and Application of Different HPLC Methods for the Separation of Hydrophobic Membrane Proteins -- Cation-Exchange High-Performance Liquid Chromatography: Group Separation of Acidic and Basic Proteins Using Volatile Solvents -- Separation of Protein Isoforms: An Ion-Exchange HPLC Separation of Bovine and Cardiac Troponin Complexes -- Assessment of Steroid Receptor Polymorphism by High-Performance Ion-Exchange Chromatography -- SECTION V: REVERSED-PHASE CHROMATOGRAPHY -- Reversed-Phase Packings for the Separation of Peptides and Proteins by Means of Gradient Elution High-Performance Liquid Chromatography -- Commercially Available Columns and Packings for Reversed-Phase HPLC of Peptides and Proteins -- Requirement for Peptide Standards to Monitor Colunm Performance and the Effect of Column Dimensions, Organic Modifiers, and Temperature in Reversed-Phase Chromatography -- The Use of Peptide Standards to Monitor Non-Ideal Behavior Due to Underivatized Silanols in Reversed-Phase Chromatography -- The Effect of Varying Flow-Rate, Gradient-Rate, and Detection Wavelength on Peptide Elution Profiles in Reversed-Phase Chromatography -- Manipulation of pH and Ion-Pairing Reagents to Maximize the Performance of Reversed-Phase Columns -- The Effects of Anionic Ion-Pairing Reagents on Peptide Retention in Reversed-Phase Chromatography -- The Biological Activity of Polypeptides after Reversed-Phase Chromatography: A Comparative Study -- Reversed-Phase Chromatography of Insulin and lodinated Insulin -- Batch-Extraction of Human Pituitary Peptides on a Reversed-Phase Matrix: Application to the Purification of the POMC-Joining Peptide.
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Separation of Intrachain Disulfide Bridged Peptides from their Reduced Forms by Reversed-Phase Chromatography -- Automated Determination of Amino Acid Enantiomers Using Derivatization with l-(9-Fluorenyl)Ethyl Chloroformate and Reversed-Phase Liquid Chromatography -- Reversed-Phase High-Performance Liquid Chromatography for the Separation of Peptide Diastereoisomers -- Chromatography of Proteins at High Organic Solvent Concentrations: An Inverse- Gradient Reversed-Phase HPLC Method for Preparing Samples for Microsequence Analysis -- Discontinuous Reversed-Phase Chromatography: A Separation Method for Complex Protein Mixtures Applicable in Both Analytical and Large Scale High-Performance Liquid Chromatography -- SECTION VI: HYDROPHOBIC INTERACTION CHROMATOGRAPHY -- Hydrophobic Interaction Chromatography of Proteins -- Effects of HPLC Solvents and Hydrophobic Matrices on Denaturation of Proteins -- High-Performance Hydrophobic Interaction Chromatography of a Labile Regulatory Protein -- SECTION VII: AFFINITY AND IMMUNOAFFINITY CHROMATOGRAPHY -- High-Performance Affinity Chromatography of Peptides and Proteins -- HPLC Purification of Plasma Serine Proteinases: Application to Plasma Kallikrein and Factor XII -- The Use of Heparin Affinity High-Performance Liquid Chromatography for the Isolation and Characterization of Fibroblast Growth Factors -- Theory and Practical Aspects of High-Performance Immunoaffinity Chromatography -- Isolation and Measurement of Membrane Proteins by High-Performance Immunoaffinity Chromatography -- SECTION VIII: COLUMN PACKING TECHNIQUES -- Packing Liquid-Chromatography Columns -- Packing Columns in the Microbore to Semi-Preparative Range -- SECTION IX: METHODS OF DETECTION -- The Practical Application of Diode Array UV-Visible Detectors to High-Performance Liquid Chromatography Analysis of Peptides and Proteins.
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Peak Identification in HPLC of Peptides Using Spectral Analysis and Second Order Spectroscopy of Aromatic Amino Acid Residues -- Detection Alternatives for HPLC Analysis of LHRH and LHRH Analogs -- Post Column Reaction Systems for Fluorescence Detection of Polypeptides -- SECTION X: ANALYSIS OF PEPTIDE AND PROTEIN CONFORMATION BY HPLC -- HPLC Analysis of Protein Folding -- Analyses of Protein Folding by Size-Exclusion Chromatography -- Complementary Application of Size-Exclusion Chromatography and Circular Dichroism to the Study of Protein Folding -- On-Line Conformational Monitoring of Proteins in HPLC -- Reversed-Phase HPLC Separation of Homologous Proteins: Interleukin-2 Muteins -- Reversed-Phase Chromatography: The Effect of Induced Conformations on Peptide Retention -- The Effect of Hydrophobic Residue Distribution in a-Helical Peptides on Peptide Retention Behavior in Reversed-Phase HPLC -- SECTION XI: INTRODUCTION TO NARROW BORE, MICROBORE, AND RAPID HPLC ANALYSIS TECHNIQUES -- Micro-HPLC Techniques for the Purification and Analysis of Peptides and Proteins -- Reversed-Phase HPLC Separation of Sub-Nanomole Amounts of Peptides Obtained from Enzymatic Digests -- Comparison of Peptide Resolution on Conventional, Narrowbore, and Microbore Reversed-Phase Columns -- Rapid HPLC of Peptides and Proteins -- Ultrafast HPLC for Protein Analysis -- SECTION XII: PREDICTION AND COMPUTER SIMULATION OF PEPTIDE AND PROTEIN SEPARATIONS AND STRUCTURE FOR RESEARCH AND TEACHING -- Development and Application of a Computer Simulation Program for Method Development and Teaching in HPLC: ProDigest-LC -- Computer Simulation as a Tool for Optimizing Gradient Separations -- HPLC Hydrophobicity Parameters: Prediction of Surface and Interior Regions in Proteins -- SECTION XIII: PREPARATIVE CHROMATOGRAPHY OF PEPTIDES AND PROTEINS.
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Practical Aspects of Preparative Reversed-Phase Chromatography of Synthetic Peptides -- Purification of Histidine-Rich Hydrophilic Peptides -- Preparative Reversed-Phase Gradient Elution Chromatography on Analytical Columns -- Preparative Reversed-Phase Shallow Gradient Approach to the Purification of Closely- Related Peptide Analogs on Analytical Instrumentation -- Preparative Reversed-Phase Sample Displacement Chromatography of Synthetic Peptides -- Displacement Chromatography of Peptides and Proteins -- SECTION XIV: ANALYSIS OF PEPTIDES AND PROTEINS -- Practical Considerations for Assessing Product Quality of Biosynthetic Proteins by HPLC -- Protein and Peptide Mapping by Two-Dimensional High-Performance Liquid Chromatography -- Amino Acid Analyses of Proteins and Peptides: An Overview -- Beckman System 6300 High-Performance Amino Acid Analyzer -- Pharmacia/LKB Amino Acid Analyzer Systems Using Pre-Column or Post-Column Derivatization -- Protein and Peptide Sequencing by Automated Edman Degradation -- Analysis of Proteins and Peptides by Free Solution Capillary Electrophoresis (CE) -- INDEXES -- Structure Index -- Index.
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