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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 190 (1993), S. 536-543 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Agroforestry systems 25 (1994), S. 193-216 
    ISSN: 1572-9680
    Keywords: trees ; paddy rice (Oryza sativa) ; Bangladesh ; participatory ; agroforestry ; farmermanaged research ; homestead forests ; village forestry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper describes the background, context and process of farmer consultation and participation in research leading to a model design for planting trees in crop fields and homesteads on small private farms in Bangladesh. Planting trees in crop fields is a cultural and technical innovation under development through participatory action-research. Tree planting on homesteads was also a high priority for farmers but poses few technical problems so was not an important topic for research. Programme execution is done by farmers themselves on researcher recommendations with technical support through small, local non-governmental organizations. The present paper provides a broad description of the programme and focusses on the process and outcome of the participatory, farmer-managed approach. It will be followed by a series of publications describing socioeconomic and technical findings of the research.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Agroforestry systems 31 (1995), S. 39-57 
    ISSN: 1572-9680
    Keywords: paddy rice ; trees in cropland ; fuelwood ; farming systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The design and methods are described of farmer-managed action-research to test the survival and performance of trees planted in paddy fields of private farms in Bangladesh. Farmers received seedlings of indigenous and exotic trees with extension advice on planting methods and care. Planting was done in systematically designed randomized modules involving twelve thousand trees up to the end of 1990. Survival and condition of individual seedlings were monitored twice annually, and mortality was replanted for up to three years after which little further mortality occurred. Stocking rate, including original survivors and replanted seedlings, tended towards 55% after six years. The main factors conductive to survival were the presence of a ‘nurse’ crop in the field at the time of tree planting, and the quality and size of planting stock. Cause of death could not be attributed in about 8% of mortality. Main recorded causes of tree mortality, in order of frequency, were livestock browsing, physical damage during cultivation or harvesting operations, pests or diseases, drought, and flooding. About 15% of tree mortality was due to losses of complete modules owing to catastrophic flooding, land disputes, and public works such as roads or pipelines. It is suspected that some of this mortality may be unacknowledged changes of mind and deliberate removal by the participating farmers.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Agroforestry systems 35 (1996), S. 1-13 
    ISSN: 1572-9680
    Keywords: canopy density ; farmer attitudes ; seasonality ; direction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seasonal yields of wetland rice and wheat were measured under traditionally-managed field trees of five species in northwestern Bangladesh over four years. There was a variable depression of rice (Oryza sativa) and wheat (Triticum aestivum) yields under all tree species. Yield depression overall ranged from 16% for the light-canopiedAcacia catechu to a little over 40% for the dense-canopiedArtocarpus heterophyllus andMangifera indica. Percentage yield depression was independent if agroecological zones, years, and location of the trees on the margins of centrally in a field. Yield depression in the dry season extended further in area than in the wet season; and for most tree species was to some extent alleviated by availability of irrigation. Farmers were well aware of and accepted crop yield losses under different tree species in return for the tree products. Trees with greatest impact on crops yielded products of highest value, mainly fruits and leaf fodder.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Rain and snow in Alberta are seldom acid. The S content of snow is so low that the snow pack gives a deposition of less than 1 kg S ha−1, even downwind from large SO2 emission sources. Rainfall contributes at the most 4 kg S ha−1 yearly near SO2 sources, and only about 1 kg S ha−1 in clean areas. However, rain intercepted by forest trees exposed to SO2 emission becomes acid (pH 3.5 to 4.5) and has a S content of 3 to 4 times greater than rain. Soils absorb large amounts of S from emissions (up to 50 kg S ha −1 annually) but much of the S is found in non-sulphate form. Soils are slowly acidified by the SO2 at a rate estimated at 1 pH unit in 10 to 20 yr. Water surfaces will absorb SO2 emissions at a rate of about 4 to 15 kg S ha−1 annually. Particulates deposit 3 to 4 times as much S as is deposited by rainfall.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 14 (1980), S. 133-157 
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This preliminary survey comprises a collection of fluoride concentration data for air, water, soil, vegetation and marine samples obtained both specifically for this paper, and from the hitherto unpublished results of others, in the vicinity of a large aluminum smelter operating at the head of Kitimat Arm, a typical Canadian west coast fjord with high rainfall. The significance of the fluoride distribution and dispersion processes derived from this data matrix and from special leaching tests using water, dilute acids, and aqueous sodium bicarbonate is considered in the light of prevailing temperature, wind and precipitation patterns with regard to the plant, animal, and marine life of the area. The most recent data available are further related to the time of complete installation of dry scrubber emission control devices to determine how ambient air, water, soil, and vegetation concentrations may be responding to the reduced air and water mass emissions rates.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2015-03-18
    Description: The evolution of pathogenic bacteria is a multifaceted and complex process, which is strongly influenced by the horizontal acquisition of genetic elements and their subsequent expression in their new hosts. A well-studied example is the RegA regulon of the enteric pathogen Citrobacter rodentium . The RegA regulatory protein is a member of the AraC/XylS superfamily, which coordinates the expression of a gene repertoire that is necessary for full pathogenicity of this murine pathogen. Upon stimulation by an exogenous, gut-associated signal, namely, bicarbonate ions, RegA activates the expression of a series of genes, including virulence factors, such as autotransporters, fimbriae, a dispersin-like protein, and the grlRA operon on the locus of enterocyte effacement pathogenicity island. Interestingly, the genes encoding RegA homologues are distributed across the genus Escherichia , encompassing pathogenic and nonpathogenic subtypes. In this study, we carried out a series of bioinformatic, transcriptional, and functional analyses of the RegA regulons of these bacteria. Our results demonstrated that regA has been horizontally transferred to Escherichia spp. and C. rodentium . Comparative studies of two RegA homologues, namely, those from C. rodentium and E. coli SMS-3-5, a multiresistant environmental strain of E. coli , showed that the two regulators acted similarly in vitro but differed in terms of their abilities to activate the virulence of C. rodentium in vivo , which evidently was due to their differential activation of grlRA . Our data indicate that RegA from C. rodentium has strain-specific adaptations that facilitate infection of its murine host. These findings shed new light on the development of virulence by C. rodentium and on the evolution of virulence-regulatory genes of bacterial pathogens in general.
    Print ISSN: 0019-9567
    Electronic ISSN: 1098-5522
    Topics: Medicine
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  • 8
    Publication Date: 2013-03-19
    Description: AraC-like regulators play a key role in the expression of virulence factors in enteric pathogens, such as enteropathogenic Escherichia coli (EPEC), enterotoxigenic E. coli , enteroaggregative E. coli , and Citrobacter rodentium . Bioinformatic analysis of the genome of rabbit-specific EPEC (REPEC) strain E22 (O103:H2) revealed the presence of a gene encoding an AraC-like regulatory protein, RegR, which shares 71% identity to the global virulence regulator, RegA, of C. rodentium . Microarray analysis demonstrated that RegR exerts 25- to 400-fold activation on transcription of several genes encoding putative virulence-associated factors, including a fimbrial operon (SEF14), a serine protease, and an autotransporter adhesin. These observations were confirmed by proteomic analysis of secreted and heat-extracted surface-associated proteins. The mechanism of RegR-mediated activation was investigated by using its most highly upregulated gene target, sefA . Transcriptional analyses and electrophoretic mobility shift assays showed that RegR activates the expression of sefA by binding to a region upstream of the sefA promoter, thereby relieving gene silencing by the global regulatory protein H-NS. Moreover, RegR was found to contribute significantly to virulence in a rabbit infection experiment. Taken together, our findings indicate that RegR controls the expression of a series of accessory adhesins that significantly enhance the virulence of REPEC strain E22.
    Print ISSN: 0019-9567
    Electronic ISSN: 1098-5522
    Topics: Medicine
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  • 9
    Publication Date: 2013-10-26
    Description: The misuse of antibiotics during past decades has led to pervasive antibiotic resistance in bacteria. Hence, there is an urgent need for the development of new and alternative approaches to combat bacterial infections. In most bacterial pathogens the expression of virulence is tightly regulated at the transcriptional level. Therefore, targeting pathogens with drugs that interfere with virulence gene expression offers an effective alternative to conventional antimicrobial chemotherapy. Many Gram-negative intestinal pathogens produce AraC-like proteins that control the expression of genes required for infection. In this study we investigated the prototypical AraC-like virulence regulator, RegA, from the mouse attaching and effacing pathogen, Citrobacter rodentium, as a potential drug target. By screening a small molecule chemical library and chemical optimization, we identified two compounds that specifically inhibited the ability of RegA to activate its target promoters and thus reduced expression of a number of proteins required for virulence. Biophysical, biochemical, genetic, and computational analyses indicated that the more potent of these two compounds, which we named regacin, disrupts the DNA binding capacity of RegA by interacting with amino acid residues within a conserved region of the DNA binding domain. Oral administration of regacin to mice, commencing 15 min before or 12 h after oral inoculation with C. rodentium, caused highly significant attenuation of intestinal colonization by the mouse pathogen comparable to that of an isogenic regA-deletion mutant. These findings demonstrate that chemical inhibition of the DNA binding domains of transcriptional regulators is a viable strategy for the development of antimicrobial agents that target bacterial pathogens.
    Print ISSN: 0021-9258
    Electronic ISSN: 1083-351X
    Topics: Biology , Chemistry and Pharmacology
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  • 10
    Publication Date: 2013-10-11
    Description: Atypical enteropathogenic Escherichia coli (aEPEC) causes endemic diarrhea, diarrheal outbreaks, and persistent diarrhea in humans, but the mechanism by which aEPEC causes disease is incompletely understood. Virulence regulators and their associated regulons, which often include adhesins, play key roles in the expression of virulence factors in enteric pathogenic bacteria. In this study we identified a transcriptional regulator, RalR, in the rabbit-specific aEPEC strain, E22 (O103:H2) and examined its involvement in the regulation of virulence. Microarray analysis and quantitative real-time reverse transcription-PCR demonstrated that RalR enhances the expression of a number of genes encoding virulence-associated factors, including the Ral fimbria, the Aap dispersin, and its associated transport system, and downregulates several housekeeping genes, including fliC . These observations were confirmed by proteomic analysis of secreted and heat-extracted surface-associated proteins and by adherence and motility assays. To investigate the mechanism of RalR-mediated activation, we focused on its most highly upregulated target operons, ralCDEFGHI and aap . By using primer extension, electrophoretic mobility shift assay, and mutational analysis, we identified the promoter and operator sequences for these two operons. By employing promoter- lacZ reporter systems, we demonstrated that RalR activates the expression of its target genes by binding to one or more 8-bp palindromic sequences (with the consensus of TGTGCACA) located immediately upstream of the promoter core regions. Importantly, we also demonstrated that RalR is essential for virulence since infection of rabbits with E22 carrying a knockout mutation in the ralR gene completely abolished its ability to cause disease.
    Print ISSN: 0019-9567
    Electronic ISSN: 1098-5522
    Topics: Medicine
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