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  • 1
    Electronic Resource
    Electronic Resource
    Immunocytochemical detection of p21 ras expression in fresh human leukaemic cells and cell lines (1989)
    Springer
    Annals of hematology 59 (1989), S. 460-463 
    Details
    ISSN: 1432-0584
    Keywords: Immunocytochemistry ; Leukaemia ; Oncogenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The expression of p21 ras proteins was investigated by immunocytochemistry in permanent cell lines and in fresh human leukaemic cells. While high and low levels of p21 ras could be detected in most of the cell lines, no significant p21 ras immunoreactivity was noted in cells of ten human acute and chronic leukaemias. Thus, notwithstanding its possible role in the initial transformation process in human leukaemias, p21 ras expression appears not to be an irrevocable requirement for the maintenance of the transformed state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349069
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of epidermal growth factor/transforming growth factor-α receptor in the human gastric mucosa (1993)
    Springer
    Virchows Archiv 423 (1993), S. 57-63 
    Details
    ISSN: 1432-2307
    Keywords: Epidermal growth factor ; Transforming growth factor-α ; Receptor ; Gastric mucosa ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Current evidence indicates that epidermal growth factor (EGF) and transforming growth factor-α (TGF-α) play a pivotal role in the maintenance of gastric mucosal integrity, via binding to a common cell-surface receptor (EGF/TGF-α receptor). We examined the distribution and cellular sites of synthesis of EGF/TGF-α receptor in normal human gastric mucosa by immunohistochemical and in situ hybridization techniques. Intense EGF/TGF-α receptor immunoreactivity was observed in the basal cytoplasm and along basolateral membranes of mucus neck cells, foveolar columnar cells, and surface epithelial cells facing the gastric lumen. Parietal cells and mucus-secreting pyloric gland cells displayed a distinct basolateral immunostaining, whereas the luminal membrane was unstained. Immunoreactivity was also noted in spindle-shaped cells of the lamina propria and in smooth muscle cells of the muscularis mucosae and muscularis propria. In situ hybridization revealed EGF/TGF-α receptor RNA transcripts in all cell types displaying positive immunoreaction. These results suggest a physiological role for EFG/TGF-α in the regulation of multiple gastric functions. The receptor distribution at the luminal aspect of the gastric mucosa provides the anatomical basis for a possible interaction of gastric juice EGF (or TGF-α) with cells of the mucosal surface, whereas the expression of EGF/TFG-α receptor in cells which are not in direct contact with the gastric lumen is consistent with blood-mediated or paracrine/ autocrine mechanisms of EGF/TGF-α action on these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606433
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  • 3
    Electronic Resource
    Electronic Resource
    Vimentin expression of newly formed rat bile duct epithelial cells in secondary biliary fibrosis (1989)
    Springer
    Virchows Archiv 415 (1989), S. 237-242 
    Details
    ISSN: 1432-2307
    Keywords: Immunohistology ; Monoclonal anti-bodies ; Vimentin ; Cytokeratin ; Intermediate filaments ; bile ducts ; Ki-67 ; Proliferation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The intermediate filament profile and the growth fraction of hepatocytes and bile duct epithelial cells were studied in a rat model of biliary fibrosis secondary to common bile duct ligation and scission. Strong vimentin expression was observed in epithelial cells of newly formed bile ductules, while normal liver contained only few weakly positive bile duct epithelial cells. All epithelial cells reacted with a pan-cytokeratin antibody. A monoclonal antibody specific for human cytokeratin 7 selectively reacted with both normal and newly formed bile duct epithelial cells. The intermediate filament profile of hepatocytes was constant, showing no changes during proliferation or in periportal areas adjacent to excessive bile duct formations. The proliferation-associated antigen detected by the antibody Ki-67 was present in many hepatocytes, homogeneously distributed in the lobules, but was seen only in a small proportion of the epithelial cells of the newly formed bile ducts. We conclude that vimentin may serve as an indicator for cellular reorganization in the bile duct system, and that the epithelial cells of newly formed bile ductules in this particular model of secondary biliary fibrosis were most likely to be derived from an outgrowth of the biliary duct system and recruitment of preductular epithelial cells. No morphological or immunohistological evidence suggesting a derivation from hepatocytes by ductular metaplasia or from oval cells was obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00724910
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of type I and IV collagen mRNAs in healing gastric ulcers – a comparative analysis using isotopic and non-radioactive in situ hybridization (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 413-418 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The sensitivity and practicability of in situ hybridization methods utilizing isotopic or non-radioactive labeling were compared. The aim of this study was to determine whether digoxigenin-labeled riboprobes are as sensitive as 35S-labeled probes to detect changes in type I and IV procollagen expression in an animal model of rat gastric ulcer. Both labeling and detection methods yielded similar results, with a superimposable signal distribution in the specimens. High levels of procollagen type I and IV transcripts were observed in spindle-shaped cells, presumably fibroblasts or myofibroblasts, localized in the ulcer base and rim. The increased expression of these collagen types suggests a remarkable upregulation of collagen expression during the healing of gastric ulcers. Liver tissue adhering to perforated ulcers displayed signals related to non-parenchymal cells, with hepatocytes demonstrating no detectable transcripts of type I or IV collagen genes. Due to the identical pattern of signal distribution by both hybridization techniques it is concluded that non-radioactive in situ hybridization is of value in monitoring highly expressed genes and yields results similar to those achieved with radioactive probes. In these cases, non-radioactive techniques are preferable because they are performed more rapidly and do not require handling of isotopes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050058
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  • 5
    Electronic Resource
    Electronic Resource
    Expression of type I and IV collagen mRNAs in healing gastric ulcers—a comparative analysis using isotopic and non-radioactive in situ hybridization (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 413-418 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The sensitivity and practicability of in situ hybridization methods utilizing isotopic or non-radioactive labeling were compared. The aim of this study was to determine whether digoxigenin-labeled riboprobes are as sensitive as35S-labeled probes to detect changes in type I and IV procollagen expression in an animal model of rat gastric ulcer. Both labeling and detection methods yielded similar results, with a superimposable signal distribution in the specimens. High levels of procollagen type I and IV transcripts were observed in spindle-shaped cells, presumably fibroblasts or myofibroblasts, localized in the ulcer base and rim. The increased expression of these collagen types suggests a remarkable upregulation of collagen expression during the healing of gastric ulcers. Liver tissue adhering to perforated ulcers displayed signals related to non-parenchymal cells, with hepatocytes demonstrating no detectable transcripts of type I or IV collagen genes. Due to the identical pattern of signal distribution by both hybridization techniques it is concluded that non-radioactive in situ hybridization is of value in monitoring highly expressed genes and yields results similar to those achieved with radioactive probes. In these cases, non-radioactive techniques are preferable because they are performed more rapidly and do not require handling of isotopes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02473300
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  • 6
    Electronic Resource
    Electronic Resource
    Glial fibrillary acidic protein and RNA expression in adult rat hippocampus following low-level lead exposure during development (1996)
    Springer
    Histochemistry and cell biology 105 (1996), S. 431-442 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The astroglial cytoskeletal element, glial fibrillary acidie protein (GFAP), is a generally accepted sensitive indicator for neurotoxic effects in the mature brain. We used GFAP as a marker for structural changes in rat hippocampus related to chronic low level lead exposure during different developmental periods. Four groups of rats were investigated: a control group, a perinatal group, which was exposed during brain development (EO-P16), a permanent group, exposed during and after brain development (E0-P100), and a postweaning group, exposed after brain development (P16–P100). Sections were processed for light microscopy (hematoxylin-eosin, Nissl, periodic acid Schiff (PAS) and GFAP-specific immunohistology), for electron microscopy, and for in-situ hybridization (GFAP). Sections were prepared from animals tested for active avoidance learning (AAL) and long-term potentiation (LTP). Chronic lead exposure did not affect glial and neuronal functions, as assessed by LTP and AAL, when lead exposure started after brain development (postweaning group). In this group, astrocytes displayed increased GFAP and GFAP gene transcript levels. However, lead exposure affected neuronal and glial function when the intoxication fell into the developmental period of the brain (perinatal and permanent groups). In these groups, LTP and AAL were impaired, and astrocytes failed to react to the toxic exposure with an adequate increase of GFAP and GFAP gene transcripts. Although GFAP is an accepted marker for neurotoxicity, our data suggest the marker function of GFAP to be restricted to postnatal toxic insult.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01457656
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