ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Description / Table of Contents:
Summary 1. Yellow fever virus (17 D-, French Neurotropic-, Asibi-strains) has been cultivated in explants of human tissue (tumor cellines HeLa and KB, normal amnion and kidney) without any previous adaptation. 2. KB-cells proved to be distinctly more suitable than all other tissue forms. In expiants of this nature, infectivity titres of log 5,0–7,0 ID50 were regularly obtained after an incubation period of 4–6 days. 3. The demonstration of virus in expiants is easy when based on the following criteria: a) the presence of macroscopically visible tissue defects (“plaques”); b) the increasing turbidity of nutritive medium; c) the suppression of cellular metabolism, and d) the microscopic demonstration of the cytopathogenic effect. The specificity of these tissue alterations is proved by the inhibitory action of yellow fever immune sera. 4. Culturing of yellow fever virus in human expiants allows an accurate titration of the infectivity. The final titre is approximately the same as that obtained using the intracerehral mouse test. Immune sera may also he evaluated in a reliable way in such expiants. 5. During the passage into KB-explants, the 17 D-strain lost its pathogenicity for mice, the Asibi-strain its viscerotropic affinity for monkeys.
Notes:
Zusammenfassung 1. Gelbfiebervirus (17 D-, French neurotropic-, Asibi-Stamm) kann in menschlichen Gewebsexplantaten (HeLa, KB, Amnion, Niere) in Dauerpassagen gezüchtet werden. Als optimal geeignet erwies sich der KB-Zellstamm. 2. Virus und Antikörper können im Explantat titriert werden. Für den Virusnachweis des 17 D-Stammes ist das Explantat ebenso empfindlich wie der cérébrale Mäusetest. 3. Bei der Dauerpassage in KB-Explantaten büßte der 17 D-Stamm seine Mäusepathogenität und der Asibi-Stamm seine viscerotropen Eigenschaften für den Affen ein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01248832
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