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  • 1
    Electronic Resource
    Electronic Resource
    FATTY ACID PROFILES OF LEAVES OF NINE EDIBLE WILD PLANTS: AN AUSTRALIAN STUDY (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food lipids 9 (2002), S. 0 
    Details
    ISSN: 1745-4522
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Nine species (Amaranthus viridis L., Atriplexnummularia L., Chenopodium album L., Plantago major L., Portulaca oleracea L., Solanum nigrum L., Sonchus oleraceus L., Stellaria media L. and Taraxacum officinale W.) of edible wild plants grown in Australia were examined for their fatty acid composition by gas chromatography. The total fatty acid contents in the young leaves of these species ranged from 8.75 to 29.12 mg/g of dry matter, and were predominantly comprised of the alpha-linolenic acid (4.78 to 19.88 mg/g). These plants did not contain any of the longer-chain omega-3 fatty acids, namely eicosapentaenoic acid, docosahexaenoic acid or docosapentaenoic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4522.2002.tb00209.x
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  • 2
    Electronic Resource
    Electronic Resource
    Increased endogenous cytokinin in the Arabidopsis amp1 mutant corresponds with de-etiolation responses (1996)
    Springer
    Planta 198 (1996), S. 549-556 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis (mutant) ; Cytokinin ; De-etiolation ; Mutant (amp1) ; Photomorphogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The high-cytokinin Arabidopsis thaliana (L.) Heynh. mutant amp1 of has been further characterised. We extend our previous work on the cytokinin level in the amp1 mutant and show that it contains high levels of endogenous cytokinins in both light- and dark-grown plants, and that amp1 can be phenocopied in the dark by growing wild-type plants on exogenous cytokinin. Previously we showed that dark-grown amp1 plants display de-etiolated characteristics such as short unhooked hypocotyls, opened cotyledons and formation of leaves. We further show that amp1 mutants display de-etiolated plastid morphology and increased levels of transcripts of light-regulated genes, indicating that in this mutant light-mediated processes are partially induced in the dark. The amp1 mutant also shows a reduced level of expression of several light-regulated genes compared with the wild type when grown in light, and has an altered dark-adaptation response when compared with the wild type. These results demonstrate an association between high cytokinin levels and de-etiolation, and we infer that cytokinin itself or a cytokinin-mediated process is involved in regulation of etiolation. The map location, phenotypes and de-etiolation responses in the amp1 mutant are different from those of previously described de-etiolated mutants such as det1, det2, cop1 and cop9. We propose a model in which cytokinin acts as a component of the induction of photomorphogenic processes via a signal transduction pathway which is independent of light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262641
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  • 3
    Electronic Resource
    Electronic Resource
    Fractionation of carbohydrates in Arabidopsis root cell walls shows that three radial swelling loci are specifically involved in cellulose production (2000)
    Springer
    Planta 211 (2000), S. 406-414 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Arabidopsis (cellulose ; mutants) ; Carbohydrate fractionation ; Cellulose synthesis ; Cell walls ; Mutant (Arabidopsis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract.  Three non-allelic radial swelling mutants (rsw1, rsw2 and rsw3) of Arabidopsisthaliana L. Heynh. were shown to be specifically impaired in cellulose production. Fractionation methods that identify, characterise and quantify some of the major cell wall polysaccharides in small quantities of seedlings demonstrated that changes in the production of cellulose are much more pronounced than changes in the production of non-cellulosic polysaccharides. A crude cell wall pellet was sequentially extracted with chloroform methanol (to recover lipids), dimethyl sulphoxide (starch), ammonium oxalate (pectins) and alkali (hemicelluloses). Crystalline cellulose remained insoluble through subsequent treatments with an acetic/nitric acid mixture and with trifluoroacetic acid. Cetyltrimethylammonium bromide precipitation resolved neutral and acidic polymers in the fractions, and precipitation behaviour, monosaccharide composition and glycosidic linkage patterns identified the major polysaccharides. The deduced composition of the walls of wild-type seedlings and the structure and solubility properties of the major polymers were broadly typical of other dicots. The three temperature-sensitive, radial swelling mutants produced less cellulose in their roots than the wild type when grown at their restrictive temperature (31 °C). There were no significant differences at 21 °C where no radial swelling occurs. The limited changes seen in the monosaccharide compositions, glycosidic linkage patterns and quantities of non-cellulosic polysaccharides support the view that the RSW1, RSW2 and RSW3 genes are specifically involved in cellulose synthesis. Reduced deposition of cellulose was accompanied by increased accumulation of starch.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250000301
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  • 4
    Electronic Resource
    Electronic Resource
    Negative-ion mass spectrometry of substituted adenine bases and adenosine nucleosides (1989)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 3 (1989), S. 249-254 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The negative-ion chemical ionization (ammonia, 5 Pa source pressure) mass spectra of a series of substituted adenine bases, adenosine nucleosides, and the trimethylsilyl derivatives of the nucleosides are described. Selected ions from these spectra were subject to collisionally activated dissociation with mass-analysed ion kinetic energy (CAD/MIKE) analysis of the products and the spectra assessed for information content. In addition to observing strong peaks due to quasimolecular ions and heterocyclic-base ions, it proved possible to differentiate between 2′-, 3′- and 5′-deoxy and between 2′- and 3′-O-methyl isomers. The negative-ion chemical ionization spectra of four methyladenines are essentially identical, but could be clearly distinguished from each other by CAD/MIKE analysis.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290030802
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  • 5
    Electronic Resource
    Electronic Resource
    Chemical archaeology of kava, a potent brew (1993)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 7 (1993), S. 219-224 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Kava lactones, which are present in the intoxicating Pacific Island drink, kava, have now been detected in a number of archaeological artefacts using selected-ion monitoring techniques in conjunction with gas chromatography/electron impact-mass spectrometry. Thus it is now possible to link unequivocally kava drinking, a major aspect of the ceremonial culture of many Pacific societies, to the archaeological record. In addition, a new variation of the kava lactone skeleton was tentatively identified in the form of 7,8-dihydro-5,6-dehydrokawain and 7,8-dihydro-5,6-dehydromethysticin.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290070311
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  • 6
    Electronic Resource
    Electronic Resource
    Negative ion mass spectrometry of silylated cytokinins (1989)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 3 (1989), S. 36-38 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The negative ion mass spectra or trimethylsilylzeatin riboside and t-butyldimethylsilylzeatin, generated using either ammonia or 10% CO2/Ar as reagent gas, are described. Optimal yields of [M-H]- ions were obtained with a source pressure of 5 Pa in the electron ionization source of a ZAB-2F mass spectrometer, compared with 15 Pa to generate [M+H]+ in the chemical ionization mode. At 15 Pa no negative ions could be observed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290030208
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