ISSN:
1432-0568
Keywords:
Cat ependymal cilia
;
Filipin
;
Cholesterol
;
Freeze-fracture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The polyene antibiotic filipin reacts specifically with membrane cholesterol and produces membrane perturbations. Prefixed vibratome slices of cat ependymal cells were treated with a glutaraldehyde solution containing 300 μM filipin. Freeze-fracture of these cells revealed numerous 25–30 nm protuberances on fracture faces (filipinsterol complexes) in the ciliary membrane. In the ciliary necklace, however, these filipin-sterol complexes were absent. These results suggest that the ciliary necklace has a low cholesterol content.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00318948
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