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  • 1
    Electronic Resource
    Electronic Resource
    Increased Levels of Neuron-Specific Enolase in PC 12 Pheochromocytoma Cells as a Result of Nerve Growth Factor Treatment (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 37 (1982), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Treatment of PC 12 pheochromocytoma cells with nerve growth factor (NGF) resulted in increased levels of neuron-specific enolase (NSE). Neither insulin, growth hormone, cytochrome c, nor sodium butyrate increased NSE levels. Epidermal growth factor (EGF) did increase NSE levels, although not to the same extent as NGF. As little as 1 ng/ml NGF induced the maximal increase in NSE. As PC 12 cells increased in density, the NSE levels increased even in untreated cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1982.tb12528.x
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  • 2
    Electronic Resource
    Electronic Resource
    POLYADENYLIC ACID-CONTAINING RNA FROM RAT BRAIN SYNAPTOSOMES (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 25 (1975), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Synaptosomal RNA of rat brain was labelled in vivo by intracranial injection of tritiated uridine. The change in the specific activity of this material with time was similar to that of polysomal RNA. The percent of the radioactive synaptosomal RNA which bound to oligo(dT)-cellulose columns decreased with time after intracranial labelling. The percent of the total synaptosomal RNA which bound to oligo(dT)-cellulose was greater than that of polysomes. The length of the polyadenylate (poly(A)) sequence of synaptosomal RNA was approximately one-half that of polysomal RNA, and about the same as that from mitochondria. Investigation of synaptosomal RNA using sucrose gradients and polyacrylamide gel electrophoresis indicated that there were several distinct species present, and that they were similar to those from the mitochondria. The poly(A)-containing RNA isolated from synaptosomes stimulated the incorporation of radioactive leucine into TCA-precipitable material in a cell-free protein synthesis system. Isolation of RNA from subsynaptosomal components indicated that most, if not all, of the synaptosomal messenger activity was localized in the synaptic mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1975.tb12239.x
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  • 3
    Electronic Resource
    Electronic Resource
    PURIFICATION OF NERVE GROWTH FACTOR ANTIBODIES BY AFFINITY CHROMATOGRAPHY (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 26 (1976), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Pure antibodies to nerve growth factor have been isolated from sheep nerve growth factorantiserum by affinity chromatography using 2.5 S nerve growth factor linked to Sepharose 4B by means of cyanogen bromide. The elution of the antibodies was accomplished either at low pH (pH 2) or by high salt concentration (4.5 wMgC12). The purity of the antibodies was established by SDS-gel electrophoresis. Their immunological activity was tested by imrnunoprecipitation and their biological activity in a tissue culture assay using embryonic chick dorsal root ganglia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1976.tb07008.x
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  • 4
    Electronic Resource
    Electronic Resource
    THE UPTAKE OF PURINES BY RAT BRAIN IN VIVO AND IN VITRO (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 20 (1973), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— The uptake of [14C]guanine and some of its [14C]-labelled derivatives into rat brain was studied in vivo and in vitro. In vivo guanine, guanosine, and hypoxanthine penetrated the brain of adult rats to a very small extent. Inosine was taken up somewhat better. In young animals, also, guanosine was taken up poorly, but guanine was taken up fairly well. When guanine was administered to adult animals, only guanine was found in the brain. In young animals, by contrast, radioactivity from guanine appeared in guanosine and in guanine nucleotides, but no free guanine was found. In vitro guanine was taken up much better and, in fact, remained mostly as guanine in slices from 10-day-old rats. The in vitro conversion of guanine to GMP and its incorporation into RNA was unimpaired by the addition of unlabelled guanosine, an indication that guanine was converted directly to GMP. The uptake of guanine in vitro was not subject to competitive inhibition or influenced by the presence of dinitrophenol. This finding suggested that guanine entered the slice by simple diffusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1973.tb00084.x
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  • 5
    Electronic Resource
    Electronic Resource
    POLYADENYLIC ACID-CONTAINING RNA FROM RAT BRAIN (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 24 (1975), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A portion of rat brain RNA contains poly(A) sequences and binds to oligo(dT)-cellulose. Young rats have a greater amount of brain RNA which contains poly(A) than do adult animals. The length of the poly(A) sequence from the brain RNA of young animals was shown to be somewhat longer than that from the RNA of adults. The RNA which bound to the oligo(dT)-cellulose was found to be large and heterogeneous, and to be almost free of ribosomal or of small mol. wt. RNAs. When the polysomal RNA which bound to the oligo(dT)-cellulose columns and that which did not were used to prime a cell-free protein synthesizing system there was a noticeable difference in their‘messenger’activity; the RNA which bound to the oligo(dT)-cellulose was much more active than the unbound material. However, in the case of the nuclear RNA there was not as great a difference between the material which was bound and that which was not bound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1975.tb11867.x
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  • 6
    Electronic Resource
    Electronic Resource
    HYPOXANTHINE-GUANINE-PHOSPHORIBOSYL-TRANSFERASE FROM RAT BRAIN (PURIFICATION, KINETIC PROPERTIES, DEVELOPMENT AND DISTRIBUTION) (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 19 (1972), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Hypoxanthine-guanine-phosphoribosyltransferase (HGPR Tase; ECC 2.4.2.8) has been purified from rat brain 650-fold to about 50 per cent purity by conventional methods. An isoenzyme pattern of at least three components is observed on DEAE-cellulose chromatography. On polyacrylamide disc electrophoresis only one sharp band of enzyme activity can be detected. The apparent Km-value determined for phosphoribosylpyrophosphate (PRPP) is about 0.2 mM. The product, GMP, and also GDP, GTP, UMP, CMP, AMP and ATP are competitive inhibitors with respect to PRPP. Inhibition by a number of other nucleotides has also been investigated. Studies on the development of enzyme activity in the brain of the young rat show that a rapid increase occurs during the first 15-20 days of life and reaches a plateau thereafter. The regional distribution of HGPRTase activity in adult rat brain is more homogenous than that reported for human brain. The enzyme is predominantly a constituent of the soluble supernatant fraction, but can also be found in carefully washed synaptosomes. An antiserum against rat brain HGPRTase obtained from rabbits inhibits this enzyme to about 30 per cent of control activity, but does not crossreact with HGPRTases from rabbit or human erythrocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1972.tb05123.x
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  • 7
    Electronic Resource
    Electronic Resource
    INCREASED CEREBRAL EXCITABILITY CAUSED BY p-CHLOROPHENYLALANINE IN YOUNG RATS (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 18 (1971), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: —Single intraperitoneal doses of p-chlorophenylalanine (p-CP; 100 mg/kg or more), lowered the threshold of flurothyl-induced seizures in 1- and 2-week old rats after 24 h, but not after 4 or 72 h. In older rats there was no change in cerebral excitability after a single dose, but the seizure threshold was lowered after several daily doses. Rats given p-CP in lower doses daily from birth exhibited lowered seizure thresholds only until 3-4 weeks of age.Depletion of brain serotonin and inhibition of liver phenylalanine hydroxylase by p-CP exhibited time courses and dose responses similar to those reported for older animals. Activity of phenylalanine hydroxylase returned to normal more rapidly than the content of serotonin after a dose of p-CP, and recovery of normal seizure threshold seemed to be more closely associated with recovery of the hepatic enzyme than with restoration of the brain amine. It appears, therefore, that hyperphenylalanemia or some consequence of it may be an important factor contributing to increased cerebral excitability in p-CP intoxication and possibly in clinical phenylketonuria and that depletion of brain serotonin may not be the only or even the predominant cause of the changes in cerebral excitability in these conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1971.tb00177.x
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  • 8
    Electronic Resource
    Electronic Resource
    BIOSYNTHESIS OF RIBONUCLEIC ACID IN RAT BRAIN SLICES (1968)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 15 (1968), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The incorporation of uridine into RNA in brain slices was studied. Optimal conditions for uridine incorporation were determined. The characteristics of the product suggest that de novo DNA-directcd synthesis of fairly high molecular weight material takes place. Incorporation into RNA of several areas of brain was studied. The incorporation was also studied as a function of the age of the animal. Finally, an apparent correlation was observed between the decrease in uridine incorporation with age and the increase of the enzyme uridine nucleosidase which hydrolyses uridine to uracil, a material which cannot be incorporated into RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1968.tb08946.x
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  • 9
    Electronic Resource
    Electronic Resource
    Effects of nerve growth factor and nerve growth factor antiserum on tyrosine hydroxylase and dihydropterine reductase activities in rat superior cervical ganglia in vim (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 28 (1977), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1977.tb10637.x
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  • 10
    Electronic Resource
    Electronic Resource
    NERVE GROWTH FACTOR AND THE ACTIVITY OF TYROSINE HYDROXYLASE IN ORGAN CULTURES OF RAT SUPERIOR CERVICAL GANGLIA (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 28 (1977), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Superior cervical ganglia from young rats were cultured in the absence of serum. The effect of nerve growth factor on the level of tyrosine hydroxylase was studied. In the absence of nerve growth factor the specific activity of tyrosine hydroxylase fell by more than 50% within 48 h. In the presence of nerve growth factor the total and specific activities were maintained and even increased in the same period. Both the 2.5 S and the 7 S forms of nerve growth factor were effective. Oxidized nerve growth factor had no effect except when present in very high concentration. Purified antibody to nerve growth factor was inhibitory. Insulin had only a slight effect in this system, but dibutyryl CAMP elevated tyrosine hydroxylase activity substantially. Propranolol inhibited the action of nerve growth factor but its action appeared to be nonspecific and unrelated to its action on the β-adrenergic receptor. Changes in the activity of dihydropteridine reductase paralleled those seen in tyrosine hydroxylase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1977.tb10635.x
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