GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Document type
Keywords
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    047
rAd-p21 Significantly Attenuates Granulation Tissue Formation and Scar Thickness In Vivo (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Excessive scarring is a significant clinical problem. p21, a cyclin-dependent kinase inhibitor, blocks cell cycle progression and attenuates proliferation of various cell types. Our in vitro rAd-p21 dose response studies of primary human dermal fibroblasts showed a 3–80 fold reduction of cell proliferation as measured by BrdU incorporation at doses of 1 × 108−3 × 109 PN/mL, respectively. Further, rAd-p21 at 3 × 109 PN/mL showed a 2-fold less procollagen I (PIP) production when compared to control virus. These in vitro data demonstrate that rAd-p21 significantly attenuates fibroproliferation and procollagen production in the target cells. In vivo, a rat PVA sponge model was used. rAd-p21, at doses of 1 × 109, 1 × 1010 and 5 × 1010 PN was delivered into sponges that had previously been treated with a granulation tissue stimulator, rAd-PDGF-B. Results showed that sponges receiving rAd-PDGF-B alone had the highest % granulation fill (40–60% fill area). Sponges that were treated with rAd-PDGF-B for the first injection followed by rAd-p21 on a second injection showed a dose-dependent decrease in % granulation fill as rAd-p21 doses increased (p 〈 0.001). On day 5 post-injection, IHC staining identified p21 protein expression only in sponges receiving rAd-p21. In addition, the quantitative measurement of cells labeled by BrdU or Ki67 demonstrated that rAd-p21 significantly attenuated cell proliferation when compared to rAd-PDGF alone in this model (p 〈 0.01). In another in vivo experiment, a rabbit excessive scar model was used. rAd-p21, at a dose of 2 × 109 PN/wound was intradermally injected into the rabbit ear wounds that had previously been treated with PDGF-BB protein. Preliminary data showed that scar thickness in rAd-p21 treated wounds was significantly decreased in comparison to wounds treated with the control virus (p 〈 0.05). These data suggest that rAd-p21 is effective in attenuating scars in the rabbit ear excessive scar model. Our in vitro and in vivo data support the further exploration of rAd-p21 as a useful therapeutic candidate for hyperproliferative diseases in patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractat.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    030
A Rabbit Ear Excessive Scarring Model Using Growth Factor Stimulators (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Research on abnormal scarring, such as excessive scars, has been hampered by the lack of relevant animal models. Recent studies suggest that TGF-β1, PDGF-BB and FGF-2 are stimulators of fibrosis or scarring. We developed a rabbit ear cutaneous excessive scarring model using the growth factor stimulators TGF-β1 (0.25, 0.5 or 1 μg per wound), PDGF-BB (1, 2 or 3 μg per wound) and FGF-2 (0.5, 1, 3 or 5 μg per wound). Three 8-mm diameter wounds were created on each ear in NZW rabbits (n = 6 wounds). The test growth factors were injected intradermally immediately after wounding. Controls consisted of PBS, BSA and wounding only without injections. Scar thickness of wounds was measured with a micrometer at days 14 and continued twice a week thereafter until sacrifice at day 28. Results showed that the greatest measurement of elevated scars was observed at day 14. Significant differences of scar thickness were observed in TGF-β1 at a dose of 0.25 μg, PDGF-BB at a dose of 3 μg, and FGF-2 at a dose of 3 μg per wound groups when compared to PBS and wounding only groups (p 〈 0.05). Interestingly, BSA, a protein stabilizer for TGF-β1 and FGF-2 proteins, also significantly elevated scar thickness at day 14 over PBS alone (p 〈 0.05). Trichrome stained tissue sections on day 28 showed an increase in cellularity and thicker epithelium in all doses of TGF-β1, PDGF-BB and FGF-2 protein treated groups when compared to wounding only, PBS or BSA treatments. In general, FGF-2 treated wounds showed more vascularity than TGF-β1 and PDGF-BB treated wounds. By contrast, PDGF-BB treated wounds showed richer collagen deposition than TGF-β1 and FGF-2 treated wounds. Results from these studies demonstrated that TGF-β1, PDGF-BB and FGF-2 contributed to the scar formation in the rabbit ear scar model. BSA may contribute in stimulating scar formation in the early stage of wound healing in this model. Enhancing elevated scar formation in the rabbit ear model will be useful in evaluating anti-scarring agents for excessive scars such as hypertrophic scars and keloids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractac.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    005
Cyclodextrin Polymer Based Biocompatible Matrix for Local Gene Delivery (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Biocompatible matrices, such as bovine collagen, have demonstrated usefulness in delivering gene therapy vectors that express growth factors to local environments for tissue repair. Unlike animal derived matrices, we have developed a new synthetic matrix consisting of a linear cyclodextrin-polyethyleneglycol co-polymer that is non-covalently cross-linked with di-adamantane-polyethyleneglycol via inclusion complex formation between adamantane and cyclodextrin (CD-Ada). We performed both in vitro and in vivo experiments for biocompatibility and localized transgene expression using a recombinant adenovirus (rAd) vector containing either the reporter gene, GFP, or the therapeutic gene, PDGF-B. In vitro results demonstrated cell migration, adenoviral transduction, and gene expression with no visible signs of toxicity in human skin fibroblasts. Qualitative gene expression from the CD-Ada containing rAd was delayed by approximately two days when compared to collagen, but the level of expression was greater over a longer period of time. In vivo experiments demonstrated gene expression after local delivery to mouse skin using rAd-GFP in CD-Ada. Again, the expression was slightly delayed but duration of expression was comparable to collagen. Expression studies using rAd-PDGF-B, were performed in the rat polyvinyl alcohol sponge model and showed comparable quantitative DNA and RNA levels between CD-Ada and collagen (DNA: 4.1 × 1010 and 4.5 × 1010 MEQ of PDGF-B/assayed sponge, respectively; RNA: 7.0 × 108 and 3.2 × 108 MEQ of PDGF-B/assayed sponge, respectively). Additionally, we explored the use of plasmid DNA with the CD-Ada matrix and observed PDGF-B expression in vivo. Our results show that this new delivery system provides a safe, efficient, and adaptable medium for both viral and non-viral gene delivery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractf.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    046
Characterization of Wound Repair Effects after rAd-p21 Treatment (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Excessive scarring is a significant clinical problem, resulting in both adverse tissue form and function and the goal of therapeutic interventions is to reduce and prevent excessive scarring. We have demonstrated that a recombinant adenovirus containing the cyclin-dependent kinase inhibitor p21 (rAd-p21), inhibited scar formation by blocking cell cycle progression and attenuated cell proliferation at the wound site (Perkins et al 2002). Recently, we have shown rAd-p21 specific antiproliferative effects on granulation tissue in vivo(Gu, et al, manuscript submitted). Safety parameters using rAd-p21 for anti-scarring may include effects on wound strength and dehiscence of the wound. We tested effects of rAd-p21 on wound strength in vivo using tensile strength as an endpoint and included comparisons with other clinically relevant antiproliferative agents. Specifically, rAd-p21 at doses from 1 × 107 to 3.8 × 1010 particle (PN) per incision was administered intradermally to linear rat incisions and assayed 14–28 days post treatment. rAd-p21 mildly reduced tensile strength at high doses (≥3 × 1010PN), whereas low to moderate doses (1 × 107 to 1 × 1010PN) had no effect. Interestingly, all rAd-p21 treated wounds regained tensile strength indistinguishable from vehicle control, 4 weeks after treatment, suggesting that rAd-p21 wounds recover with time. An adenovirus control vector, not containing a gene (rAd-Empty), showed subtle reduction of tensile strength that was only statistically significant at day 21. This suggests that delivery of rAd-Empty alone in the wound has little effect on wound strength. Triamcinolone at 5 mg/mL/wound, 5-FU at 10 mg/mL/wound, and low doses of MMC did not significantly reduce tensile strength, although high doses of MMC (0.2 mg/mL/wound) severely reduced tensile strength which failed to recover after 28 days. Morphological analysis of all groups revealed necrosis only in the MMC treatment. This data suggests that rAd-p21 may reduce the hyperproliferative status in excessive scar formation with minimal effects on wound strength.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractas.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Optical recording using copper phthalocyanine thin films
    Amsterdam : Elsevier
    Solid State Electronics 37 (1994), S. 1768-1770 
    Details
    ISSN: 0038-1101
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0038-1101(94)90226-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    The reaction of mercaptans with dimethyldioxirane. A facile synthesis of alkanesulfinic acids.
    Amsterdam : Elsevier
    Tetrahedron Letters 34 (1993), S. 67-70 
    Details
    ISSN: 0040-4039
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0040-4039(00)60059-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Equilibrium cation binding constants of the dipeptide derived lariat ethers in methanol determined from circular dichroism measurements
    Amsterdam : Elsevier
    Tetrahedron Letters 35 (1994), S. 681-684 
    Details
    ISSN: 0040-4039
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0040-4039(00)75789-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Expression of human PAI-2 in the baculovirus expression system (1998)
    Springer
    Journal of industrial microbiology and biotechnology 21 (1998), S. 175-177 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: plasminogen activator inhibitor-2; baculovirus; expression vector; secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Using pSXIVVI+X3 as an expressing vector, an occluded recombinant Trichoplusia ni nuclear polyhedrosis virus carrying the cDNA encoding plasminogen activators inhibitor-2 (PAI-2) under the control of the Syn and XIV promoters, has been constructed. SDS-PAGE and immunoblot analysis revealed that the virus-mediated PAI-2, with a molecular weight of ∼45 kDa, was synthesized in the Sf cells at a level of ∼16% of total intracellular protein and in the supernatant phase at a level of ∼64% of total extracellular protein secreted into the hemolymph of infected larvae. The expressed protein was similar to its authentic counterpart in terms of immunoreactivity and bioactivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900559
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Expression of the fusion protein recombinant human granulocyte-macrophage colony stimulating factor and leukemia inhibitory factor in a baculovirus vector system (1999)
    Springer
    Journal of industrial microbiology and biotechnology 23 (1999), S. 103-105 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: GM-CSF; LIF; baculovirus system; transfer vector; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A fusion gene coding human granulocyte-macrophage colony stimulating factor (GM-CSF) and leukemia inhibitory factor (LIF) cDNAs was inserted into the transfer vector pSXIVVI+ X3 with the control of Syn and XIV promoters. The Sf9 cells (Spodoptera frugiperda) were co-transfected with the recombinant plasmid and TnNPV DNA (Trichoplusia ni nuclear polyhedrosis virus DNA). The fusion protein recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) and leukemia inhibitory factor (LIF) could be synthesized in cells infected with recombinant virus at a level of about 23% of their total cellular protein. Activity analysis of the fusion protein in infected cells revealed that it exhibited the dual activities of GM-CSF and LIF. Western blot analysis of the expressed fusion protein in infected larvae showed that the virus-mediated fusion protein, with a molecular weight of ∼35 kDa, is confirmed with immunoreactivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900699
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Semiconducting TlSr2RCu2O7 (R=rare earth) and its superconducting derivatives (1991)
    Springer
    The European physical journal 84 (1991), S. 349-352 
    Details
    ISSN: 1434-6036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Semiconducting TlSr2RCu2O7 (R=Pr or Er) with a 1212-type structure has been synthesized in the single-phase form. Partial substitution of Sr2+ for R3+ converts this semiconductor to a 90 K superconductor TlSr2(R1−y Sr y )Cu2O7. A combination substitution, Sr2+ for R3+ and Pb4+ for Tl3+, leads to the Ca-free 100 K superconductor (Tl, Pb)Sr2(R, Sr)Cu2O7. The results are explained in the framework of the mixed Cu2+/Cu3+ valence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314007
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...