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  • 1
    Online Resource
    Online Resource
    KMU-innovativ - Verbundprojekt: Entwicklung eines innovativen Schnelltests zur Diagnose der Glaukomerkrankungen (LFT-Glaukom); Teilvorhaben: Autoantikörper-Analyse und Validierung mittels Mikroarray-Technologie : Schlussbericht : Laufzeit des Vorhabens: 01.09.2017 bis 31.12.2021 (2022)
    Mainz : Universitätsmedizin Mainz
    Details Availability
    Keywords: Forschungsbericht ; Glaukom ; Bedside-Methode ; Schnelltest ; Immunologie
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (25 Seiten, 912,86 KB) , Diagramme
    URL: https://doi.org/10.2314/KXP:1853073695
    URL: https://edocs.tib.eu/files/e01fb23/1853073695.pdf
    DOI: 10.2314/KXP:1853073695
    Language: German , English
    Note: Förderkennzeichen BMBF 13GW0193B , Verbundnummer 01181074 , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden , Sprache der Zusammenfassungen: Deutsch, Englisch
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  • 2
    Electronic Resource
    Electronic Resource
    Immunomodulating activity of allopurinol in experimental lens-induced uveitis (1997)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 235 (1997), S. 118-123 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract • Purpose: The aim of this study was to evaluate the immunomodulating activity of allopurinol using a model of lens-induced uveitis (LIU) and to compare these effects to those of steroids. • Methods: We tested the sera of both LIU and control rats against western blots (WB) of SDS-PAGE separations of protein fractions from normal and LIU rat lenses. These blots were scanned using digital image analysis. A newly developed technique was used to compare the complex autoantibody (AAB) repertoires. Five groups of LIU rats were investigated: no treatment; single doses of methylprednisolone (MPR; 7.5 mg/kg body wt. i.v.); allopurinol (AL; 50 mg/kg body wt. i.v.); a combination of both drugs (AL and MPR); repeated application of AL (ALFR; 50 mg/kg body wt. i.v. every 2 weeks during the immunization period and a daily dose of approx. 25 mg/kg body wt. orally). • Results: Immunization induced complex antibody repertoires against lens proteins. Antibody repertoires of LIU rats were identical, regardless of whether the proteins were obtained from control, uveitis eyes, or corresponding healthy eyes of the same individual. AL showed a dose-dependent immunological effect in LIU treatment. Given as a single dose, AL revealed no significant change in the AAB repertoire; however, ALFR showed very clear modification of the AAB repertoires compared to both controls and rats receiving steroids. • Conclusions: These results suggest dose-dependent effects of allopurinol in LIU treatment. Repeated application during the immunization period induced a strong immunomodulating effect of AL that was not observed after single doses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00941740
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  • 3
    Electronic Resource
    Electronic Resource
    Oxidative reactions in the tear fluid of patients suffering from dry eyes (1995)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 233 (1995), S. 694-698 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract • Purpose: To evaluate whether products of oxidative and inflammatory reactions are detectable in the tear fluid of patients suffering from dry eyes. • Methods: The tear fluid of 217 patients (397 eyes) was sampled. Criteria for grouping of the patients were (1) basic secretion test (“sicca l”: BST = 0–5 mm, n = 78 eyes; “sicca 2”: BST = 6–10 mm, n = 109 eyes) and (2) subjective symptoms (normal BST, burning, foreign body sensations, tearing, dryness of the eyes: n = 78 eyes). One group of healthy patients (normal BST, n = 132 eyes) served as controls. Lipid peroxide levels and myeloperoxidase activity, as parameters for oxidative tissue damage and inflammatory activity, were determined in the tear fluid. Those patients whose consent could be obtained were subjected to the rose bengal test (sicca 1: 56 eyes; sicca 2: 97 eyes; subjective symptoms: 44 eyes; controls: 49 eyes). The correlation between BST and rose bengal test results was calculated. • Results: Lipid peroxides were significantly (P 〈 0.05) higher in the groups sicca 1 and subjective symptoms than in healthy controls, as was the inflammatory activity in groups sicca 1, sicca 2 and subjective symptoms. Additionally, the inflammatory activity in the group sicca 1 was significantly (P 〈 0.05) higher than in the groups sicca 2 and subjective symptoms. No evidence of a significant correlation between BST and rose bengal test results was observed. • Conclusions: Both oxidative tissue damage and polymorphonuclear leukocytes indicating an oxidative potential occur in the tear film of patients suffering from dry eyes. These reactions lead to severe damage of the involved tissue. Free radicals and inflammation may be involved in the pathogenesis or in the self-propagation of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00164671
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  • 4
    Electronic Resource
    Electronic Resource
    Multivariate statistical comparison of autoantibody-repertoires (Western blots) by discriminant analysis (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 941-947 
    Details
    ISSN: 0173-0835
    Keywords: Western blotting ; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis ; Discriminant analysis ; Autoimmunity ; Natural autoantibodies ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A procedure for the quantification and comparison of complex (auto-) antibody repertoires of many individuals is described. It is based on multivariate statistical analyses of densitometric data of individual staining patterns, in this case Western blots. Implementation of algorithms can be based on standard software and hardware components. This procedure allows (i) the quantitative assessment of the reproducibility and reliability of any electrophoretic procedure, such as for the production of the immunological substrate, (ii) the statistical comparison of any group of staining patterns, e.g., derived from patients with autoimmune diseases or normal controls, (iii) the identification of the bands that contribute most to the differences between such groups, and (iv) the determination whether an unknown individual sample belongs to a known group. A statistical analysis of autoantibody repertoires has important possible applications: it detects spontaneous or therapeutically induced changes of repertoires, it identifies regions of interest, it supports the isolation of relevant antigens, and it will also be helpful in the diagnosis of autoimmune diseases.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601158
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  • 5
    Electronic Resource
    Electronic Resource
    Identification and classification of autoantibody repertoires (Western blots) with a pattern recognition algorithm by an artificial neural network (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 1120-1125 
    Details
    ISSN: 0173-0835
    Keywords: Polyacrylamide gel electrophoresis ; Western blotting ; Immunoblots ; Pattern recognition ; Artificial intelligence ; Neural networks ; Atree ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The screening of sera for autoantibodies with Western blots reveals complex repertoires. The composition of such repertoires depends on genetic control of autoantibody-producing cells, the individual's history of exposure to its own and to foreign antigens, and also on the presence of autoimmune diseases. Our method shows how staining patterns of Western blots can be recoded as binary or grey-value vectors. Vectors are transferred to artificial neural networks for learning. Artificial neural networks are able to recognize group-specific antibody binding patterns. Staining patterns can be attributed to diagnostic groups. This may support diagnostic procedures.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180716
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  • 6
    Electronic Resource
    Electronic Resource
    Computer-supported analysis (MegaBlot) of allopurinol-induced changes in the autoantibody repertoires of rats suffering from experimental lens-induced uveitis (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 516-519 
    Details
    ISSN: 0173-0835
    Keywords: Uveitis ; Allopurinol ; Western blot ; MegaBlot ; Lens-induced uveitis ; Artificial neural network ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Using a new quantitative immunoblot technique (MegaBlot and artificial neural network) sera of rats with lens-induced uveitis (LIU) were tested against Western blots (WB) of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separations of protein fractions from rat lenses. The blots were scanned using digital image analysis and evaluated by multivariate analysis of discriminance and an artificial neural network. Five groups of LIU rats (male Wistar rats, n = 28) were investigated: no treatment, single doses of methylprednisolone (MPR; 7.5 mg/kg body weight, intravenous), allopurinol (AL; 50 mg/kg body weight, intravenous), a combination of both drugs (AL and MPR), and repeated application of AL (ALFR; 50 mg/kg body weight intravenous every 2 weeks during the immunization period and a daily dose of approximately 25 mg/kg body weight, orally). ALFR, MPR, and MPRAL show statistically significant immunological effects in LIU treatment Classification of WBs using both discriminance analysis or neural network resulted in 100% correct assignment; and 82% (discriminance analysis) and 86% (neural network) of WBs were correctly assigned using only unknown blots for classification (not studied before). The MegaBlot technique is able to detect therapeutically induced changes in autoantibody repertoires allowing therapeutic control and a prognostic view of treatment.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180330
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