Description: Ocean acidification (OA) is a serious consequence of climate change with complex organism-to-ecosystem effects that have been observed through field observations but are mainly derived from experimental studies. Although OA trends and the resulting biological impacts are likely exacerbated in the semi-enclosed and highly populated Mediterranean Sea, some fundamental knowledge gaps still exist. These gaps are at tributed to both the uneven capacity for OA research that exists between Mediterranean countries, as well as to the subtle and long-term biological, physical and chemical interactions that define OA impacts. In this paper, we systematically analyzed the different aspects of OA research in the Mediterranean region based on two sources: the United Nation’s International Atomic Energy Agency’s (IAEA) Ocean Acidification International Coordination Center (OA-ICC) database, and an extensive survey. Our analysis shows that 1) there is an uneven geographic capacity in OA research, and illustrates that both the Algero-Provencal and Ionian sub-basins are currently the least studied Mediterranean areas, 2) the carbonate system is still poorly quantified in coastal zones, and long-term time-series are still sparse across the Mediterranean Sea, which is a challenge for studying its variability and assessing coastal OA trends, 3) the most studied groups of organisms are autotrophs (algae, phanerogams, phytoplankton), mollusks, and corals, while microbes, small mollusks (mainly pteropods), and sponges are among the least studied, 4) there is an overall paucity in socio-economic, paleontological, and modeling studies in the Mediterranean Sea, and 5) in spite of general resource availability and the agreement for improved and coordinated OA governance, there is a lack of consistent OA policies in the Mediterranean Sea. In addition to highlighting the current status, trends and gaps of OA research, this work also provides recommendations, based on both our literature assessment and a survey that targeted the Mediterranean OA scientific community. In light of the ongoing 2021-2030 United Nations Decade of Ocean Science for Sustainable Development, this work might provide a guideline to close gaps of knowledge in the Mediterranean OA research.

Description: A detailed assessment of the respective roles of production, export, and subsequent preservation of organic carbon (Corg) in the eastern Mediterranean (EMED) sediments during the formation of sapropels remains elusive. Here we present new micropaleontological results for both surface samples taken at several locations in the EMED and last interglacial sapropel S5 from core LC21 in the southeastern Aegean Sea. A strong exponential anticorrelation between relative abundances of the lower photic zone coccolithophore Florisphaera profundain the surface sediments and modern concentrations of chlorophyll a (Chl-a) at the sea surface suggests thatF. profunda percentages can be used to track past productivity changes in the EMED. Prior to S5 deposition, an abrupt and large increase of F. profunda percentages in LC21 coincided (within the multidecadal resolution of the records) with the marked freshening of EMED surface waters. This suggests a strong coupling between freshwater-bound surface to intermediate water (density) stratification and enhanced upward advection of nutrients to the base of the photic zone, fuelling a productive deep chlorophyll maximum (DCM) underneath a nutrient-starved surface layer. Our findings imply that (at least) at the onset of sapropel formation physical and biogeochemical processes likely operated in tandem, enabling high Corg accumulation at the seafloor.

Description: We investigate the long-term stability of El Niño-Southern Oscillation and Pacific Decadal Oscillation based on the examination of coccolithophore assemblages in a largely laminated 35 ka sedimentary record, retrieved in the Santa Barbara Basin (core MD02-2503). At a centennial scale coccolith assemblages indicate low primary production in the basin from 35 to 11.5 ka B.P., whereas the Holocene is characterized by high-productivity conditions. This pattern demonstrates the influence of the glacial-interglacial cycles on productivity and, by inference, on the nutrient supply by the upwelling cell off Point of Conception. On a shorter scale, laminations associated with Dansgaard-Oeschger events appear to be due to an injection of poorly oxygenated waters in the deepest part of the basin rather than anoxia due to high primary production. A seasonal sampling in seven laminated sections (spanning from 20 to 220 years) extracted from Holocene, Bølling-Allerød, and Dansgaard-Oeschger event 3 indicates El Niño probably existed continuously during the last 28 ka. The frequency of El Niño varied through time (between 1/2.5 and 1/5 event/a) and appearing to follow the precession cycle. El Niño exhibits higher (lower) frequencies when the precession values are lower (higher). Finally, the Holocene is characterized by a decrease in El Niño's frequencies due to the reinforcement of El Niño through this period.

Description: To understand the response of marine calcifying organisms under high CO2 scenarios, it is critical to study their calcification patterns in the natural environment. This paper focuses on a major calcifying phytoplankton group, the coccolithophores, through the interpretation of water samples collected along a W-E Mediterranean transect during two research cruises, in April 2011 (Meteor cruise M84/3) and May 2013 (MedSeA cruise 2013). The Mediterranean Sea is a marginal sea characterized by large biogeochemical gradients. Currently, it is undergoing both warming and ocean acidification, processes which are rapidly modifying species distribution and calcification. The species Emiliania huxleyi largely dominates the total coccolithophore production in the Mediterranean Sea. A series of morphometric measurements were performed on the coccoliths of this species to estimate their mass, length and calculate a calcification index (proxy for the size-normalized calcification degree). The most abundant morphotype of E. huxleyi in the Mediterranean Sea is Type A. Coccoliths of this morphotype were additionally analyzed based on scanning electron microscopy images: four calcification varieties were quantified, according to the relationship between slit length - tube width, and the state of the central area (open or closed). The average E. huxleyi coccolith mass along the Mediterranean oceanographic transect depended strongly on both the average coccolith length and calcification index. The variability in average coccolith length and calcification index across samples reflected oscillations in the relative abundance of the calcification varieties. We also demonstrated that the distribution of the calcification varieties followed the main environmental gradients (carbonate chemistry, salinity, temperature, nutrient concentrations). Hence, shifts in the distribution of the calcification varieties and of the average E. huxleyi coccolith mass are to be expected in the Mediterranean Sea under climate change. These physiological and ecological responses will modulate the net coccolithophore calcification and, ultimately, the regional carbonate export to the seafloor.

Description: We estimated E. huxleyi particulate inorganic to organic carbon ratio (PIC:POC) in order to detect changes in the carbonate counter-pump to carbon pump activity, which can act as either a positive or negative feedback to atmospheric CO2 modulating air-sea gas exchange.

Description: The Gulf of Sirte is a largely unexplored area in the southernmost Mediterranean Sea. We are presenting here 2008 summer data on the distribution pattern of living coccolithophores, a main phytoplankton calcifying group, in 105 samples from 20 stations. The survey includes coastal and offshore stations, enabling us to provide indications on the dynamics of phytoplankton productivity in relation to oceanographic processes. The total coccosphere concentrations show higher values in the offshore stations and lower ones for coastal sites. Umbellosphaera tenuis, Emiliania huxleyi, Florisphaera profunda, Syracosphaera pulchra HOL (Calyptrosphaera oblonga) and Rhabdosphaera spp. dominate the assemblages. The coccolithophore community shows the typical vertical zonation, with K-strategist taxa (among others U. tenuis, Rhabdosphaera spp. and Discosphaera tubifera) in the upper 75 meters depth and Lower Photic Zone taxa, dominated by F. profunda, below. The latter species shows its maximum development in the Deep Chlorophyll Maximum layer, which occurs in the upper part of the Levantine Intermediate Water, where the nutrient content is higher than in the overlying layers. The mesoscale oceanographic circulation significantly affects the spatial and vertical distribution of coccolithophores, with the thermocline and halocline depth shaping the vertical zonation of coccolithophore taxa and resulting in a strong lateral gradient within the gulf: in the eastern sector, under the influence of the Gulf of Sirte anticyclone, the DCM is deeper and so is the occurrence of K-strategist taxa and F. profunda.

Description: Coccoliths relative abundances (%) were analyzed spanning the last 30 ky at Site ODP 1238 (1°52.310′S, 82°46.934′W; 2203 m; 2203 m) in the EEP.