GLORIA — GEOMAR Library Ocean Research Information Access

1

Electronic Resource

Influence of elevated CO2 and nitrogen supply on the carbon assimilation performance and cell composition of the unicellular alga Dunaliella viridis (2003)

Gordillo, Francisco J. L. ; Jiménez, Carlos ; Figueroa, Félix L. ; [et al.]

Oxford, UK : Munksgaard International Publishers

Physiologia plantarum 119 (2003), S. 0

add to mindlist on the mindlist

Details

ISSN: 1399-3054

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The carbon assimilation efficiency and the internal composition of the chlorophyte Dunaliella viridis have been studied under conditions of current (0.035%) and enriched (1%) levels of CO2, with and without N limitation (supplied as nitrate). Results show that both photosynthesis and growth rates are enhanced by high CO2, but the strategy of acclimation also involves the light harvesting machinery and the nutritional metabolism in an N supply dependent manner. D. viridis carried out a qualitative rather than a quantitative acclimation of the light harvesting system leading to increased PSII quantum yields. Total internal C decreased as a consequence of either active growth or organic carbon release to the external medium. The latter process allowed photosynthetic electron transport to proceed at higher rates than under normal CO2 conditions, and maintained the internal C:N balance in a narrow range (under N sufficiency). N limitation generally prevented the effects of high CO2, with some exceptions such as the photosynthetic O2 evolution rate.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1399-3054.2003.00192.x

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

2

Electronic Resource

Effects of light intensity, CO2 and nitrogen supply on lipid class composition of Dunaliella viridis (1998)

Gordillo, Francisco J. L. ; Goutx, Madeleine ; Figueroa, Felix L. ; [et al.]

Springer

Journal of applied phycology 10 (1998), S. 135-144

add to mindlist on the mindlist

Details

ISSN: 1573-5176

Keywords: Light ; CO2 ; nitrogen ; lipids ; thin layer chromatography ; flame ionisation ; microalga ; batch culture ; Dunaliella viridis

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Lipid class composition of Dunaliella viridis Teodoresco was analysed using thin layer chromatography coupled with flame ionisation detection (TLC/FID technique). D. viridis was cultured under four different photon fluence rates and in darkness, and under two different conditions of CO2 supply (atmospheric and 1%) with and without nitrogen sufficiency. Nine lipid classes were identified and quantified. Total lipids per cell and acetone-mobile polar lipids decreased with light, while the percentage of sterols and triglycerides increased with increasing irradiance. Total phospholipids increase was related with growth rate while hydrocarbons, wax esters and sterol esters accumulated in darkness. There were almost no changes in total lipids per cell because of nitrogen limitation; however, nitrogen limitation led to higher changes in lipid class composition under 1% CO2 than under atmospheric CO2 levels. The main reserve lipid, triglycerides, accumulated in high amounts under 1% CO2 and nitrogen limitation, increasing from 1% to 22% of total lipids. The ratio sterols/acetone-mobile polar lipids could be an index of the 'light status' independently of nitrogen limitation, while the ratio triglycerides/total phospholipids could indicate any physiological stress uncoupling C and N metabolism and affecting the growth rate.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008067022973

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

3

Unknown

Increased CO2 modifies the carbon balance and the photosynthetic yield of two common Arctic brown seaweeds: Desmarestia aculeata and Alaria esculenta (2015)

Iñiguez, Concepcion ; Carmona, Raquel ; Lorenzo, M Rosario ; [et al.]

PANGAEA

In: Supplement to: Iñiguez, Concepcion; Carmona, Raquel; Lorenzo, M Rosario; Niell, F Xavier; Wiencke, Christian; Gordillo, Francisco J L (2016): Increased CO2 modifies the carbon balance and the photosynthetic yield of two common Arctic brown seaweeds: Desmarestia aculeata and Alaria esculenta. Polar Biology, 39(11), 1979-1991, https://doi.org/10.1007/s00300-015-1724-x

add to mindlist on the mindlist

Details

Publication Date: 2024-03-15

Description: Ocean acidification affects with special intensity Arctic ecosystems, being marine photosynthetic organisms a primary target, although the consequences of this process in the carbon fluxes of Arctic algae are still unknown. The alteration of the cellular carbon balance due to physiological acclimation to an increased CO2 concentration (1300 ppm) in the common Arctic brown seaweeds Desmarestia aculeata and Alaria esculenta from Kongsfjorden (Svalbard) was analysed. Growth rate of D. aculeata was negatively affected by CO2 enrichment, while A. esculenta was positively affected, as a result of a different reorganization of the cellular carbon budget in both species. Desmarestia aculeata showed increased respiration, enhanced accumulation of storage biomolecules and elevated release of dissolved organic carbon, whereas A. esculenta showed decreased respiration and lower accumulation of storage biomolecules. Gross photosynthesis (measured both as O2 evolution and 14C fixation) was not affected in any of them, suggesting that photosynthesis was already saturated at normal CO2 conditions and did not participate in the acclimation response. However, electron transport rate changed in both species in opposite directions, indicating different energy requirements between treatments and species specificity. High CO2 levels also affected the N-metabolism, and 13C isotopic discrimination values from algal tissue pointed to a deactivation of carbon concentrating mechanisms. Since increased CO2 has the potential to modify physiological mechanisms in different ways in the species studied, it is expected that this may lead to changes in the Arctic seaweed community, which may propagate to the rest of the food web.

Keywords: Alaria esculenta; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Arctic; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, total; Carbon, total, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carbon fixation rate; Carbon fixation rate, standard deviation; Chromista; Coast and continental shelf; Desmarestia aculeata; Dissolved organic carbon release rate; Dissolved organic carbon release rate, standard deviation; Electron transport rate; Electron transport rate, standard deviation; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross photosynthesis rate, oxygen; Gross photosynthesis rate, standard deviation; Growth rate; Growth rate, standard deviation; Initial slope of rapid light curve; Initial slope of rapid light curve, standard deviation; Irradiance; Irradiance, standard deviation; KongsfjordenOA; Laboratory experiment; Light saturation; Light saturation, standard deviation; Macroalgae; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Net photosynthesis rate, oxygen; Net photosynthesis rate, standard deviation; Nitrogen, standard deviation; Nitrogen, total; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon release rate; Particulate organic carbon release rate, standard deviation; Percentage; pH; pH, standard deviation; Photosynthetic quotient; Photosynthetic quotient, standard deviation; Polar; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Ratio; Ratio, standard deviation; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Single species; Species; Temperature, water; Temperature, water, standard deviation; Treatment; δ13C; δ13C, standard deviation

Type: Dataset

Format: text/tab-separated-values, 292 data points

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

DATA PANGAEA

Fulltext

4

Unknown

Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta (2014)

García-Gómez, Candela ; Gordillo, Francisco J L ; Palma, Armando ; [et al.]

PANGAEA

add to mindlist on the mindlist

Details

Publication Date: 2024-03-15

Description: The effects of increased CO2 and irradiance on the physiological performance of the chlorophyte Dunaliella tertiolecta were studied at different PAR and UVR (UVA+UVB) irradiances, simulating the solar radiation at different depths, under present (390 ppmv, LC) and predicted CO2 levels for the year 2100 (1000 ppmv, HC). Elevated CO2 resulted in higher optimum and effective quantum yields (Fv/Fm and Phi PSII, respectively), electron transport rates (ETR) and specific growth rates (µ). Cell stress was alleviated in HC respect to LC as evidenced by a decrease in reactive oxygen species (ROS) accumulation. DNA damage showed a 42-fold increase in cyclobutane-pyrimidine dimers (CPDs) formation under the highest irradiance (1100 µmol quanta/m**2/s) in LC with respect to the lowest irradiance (200 µmol quanta/m**2/s). Photolyase (CII-PCD-PL) gene expression was upregulated under HC resulting in a drastic decrease in CPDs accumulation to only 25% with respect to LC. Proliferating cell nuclear antigen (PCNA) accumulation was always higher in HC and the accumulation pattern indicated its involvement in repair or growth depending on the irradiance dose. The repressor of silencing (ROS1) was only marginally involved in the response, suggesting that photoreactivation was the most relevant mechanism to overcome UVR damage. Our results demonstrate that future scenarios of global change result in alleviation of irradiance stress by CO2–induced photoprotection in D. tertiolecta.

Keywords: Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyta; Cyclobutane pyrimidine dimers accumulation; Dunaliella tertiolecta; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression; Gene expression (incl. proteomics); Growth/Morphology; Growth rate; Irradiance; Laboratory experiment; Laboratory strains; Light; Macroalgae; Maximum quantum yield of photosystem II; Maximum quantum yield of photosystem II, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Plantae; Position; Primary production/Photosynthesis; Proliferating cell nuclear antigen accumulation; Reactive oxygen species; Registration number of species; Replicate; Repressor of silencing accumulation; Salinity; Single species; Species; Temperature, water; Time point, descriptive; Treatment; Type; Uniform resource locator/link to reference

Type: Dataset

Format: text/tab-separated-values, 696 data points

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

DATA PANGAEA

Fulltext

5

Unknown

Increased temperature, rather than elevated CO2, modulates the carbon assimilation of the Arctic kelps Saccharina latissima and Laminaria solidungula (2016)

Iñiguez, Concepcion ; Carmona, Raquel ; Lorenzo, M Rosario ; [et al.]

PANGAEA

In: Supplement to: Iñiguez, Concepcion; Carmona, Raquel; Lorenzo, M Rosario; Niell, F Xavier; Wiencke, Christian; Gordillo, Francisco J L (2016): Increased temperature, rather than elevated CO2, modulates the carbon assimilation of the Arctic kelps Saccharina latissima and Laminaria solidungula. Marine Biology, 163(12), 18 pp, https://doi.org/10.1007/s00227-016-3024-6

add to mindlist on the mindlist

Details

Publication Date: 2024-03-15

Description: Ocean acidification and warming are affecting with special intensity the Arctic Ocean. Arctic coastal ecosystems are dominated by kelp forests with a high biomass production, which are expected to be directly affected by the increases in CO2 and temperature. This study presents the different physiological responses of the Arctic kelps Saccharina latissima and Laminaria solidungula from Kongsfjorden (Svalbard) cultured at 4 and 9 °C in combination with current (390 ppm) and increased (1200 ppm) levels of atmospheric CO2. Both species were largely unaffected by increased CO2 conditions. Carbon fixation was not influenced by CO2, indicating that photosynthesis was C-saturated at present levels, and 13C isotopic discrimination values from algal tissue suggested no deactivation of carbon concentrating mechanisms at increased CO2 levels. Inhibition of photosynthesis by carbonic anhydrases (CAs) inhibitors highlighted the important role of external CAs in inorganic carbon acquisition in Arctic kelps. Saccharina latissima showed a significantly higher growth rate at 9 °C than at 4 °C, probably due to the decrease in the dark respiration rate observed. Growth rate of L. solidungula was not affected by temperature or CO2, and increases in photosynthesis at 9 °C could be partially related to a higher dissolved organic carbon release rate. The photochemical performance of both species was not altered by any of the treatments. These results suggest that S. latissima might be more benefited than L. solidungula in a future warmer Arctic, while both populations seem to be resilient to higher CO2 concentrations.

Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Arctic; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carbon fixation rate; Carbon fixation rate, standard deviation; Chromista; Coast and continental shelf; Dissolved organic carbon release rate; Dissolved organic carbon release rate, standard deviation; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross photosynthesis rate, oxygen; Gross photosynthesis rate, standard deviation; Growth/Morphology; Growth rate; Growth rate, standard deviation; Inhibition of carbon fixation; Inhibition of carbon fixation, standard deviation; Inhibition of oxygen evolution; Inhibition of oxygen evolution, standard deviation; KongsfjordenOA; Laboratory experiment; Laminaria solidungula; Macroalgae; Net photosynthesis rate, oxygen; Net photosynthesis rate, standard deviation; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Polar; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Registration number of species; Respiration; Respiration rate, oxygen; Respiration rate, standard deviation; Saccharina latissima; Salinity; Salinity, standard deviation; Single species; Species; Temperature; Temperature, water; Temperature, water, standard deviation; Type; Uniform resource locator/link to reference

Type: Dataset

Format: text/tab-separated-values, 432 data points

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

DATA PANGAEA

Fulltext

6

Unknown

Seawater carbonate chemistry and photoinhibition in Desmarestia anceps (2017)

Iñiguez, Concepcion ; Heinrich, Sandra ; Harms, Lars ; [et al.]

PANGAEA

In: Supplement to: Iñiguez, Concepcion; Heinrich, Sandra; Harms, Lars; Gordillo, Francisco J L (2017): Increased temperature and CO2 alleviate photoinhibition in Desmarestia anceps: from transcriptomics to carbon utilization. Journal of Experimental Botany, 68(14), 3971-3984, https://doi.org/10.1093/jxb/erx164

add to mindlist on the mindlist

Details

Publication Date: 2024-03-15

Description: Ocean acidification and warming are affecting polar regions with particular intensity. Rocky shores of the Antarctic Peninsula are dominated by canopy-forming Desmarestiales. This study investigates the physiological and transcriptomic responses of the endemic macroalga Desmarestia anceps to a combination of different levels of temperature (2 and 7 °C), dissolved CO2 (380 and 1000 ppm), and irradiance (65 and 145 µmol photons/m**2/s). Growth and photosynthesis increased at high CO2 conditions, and strongly decreased at 2 °C plus high irradiance, in comparison to the other treatments. Photoinhibition at 2 °C plus high irradiance was evidenced by the photochemical performance and intensive release of dissolved organic carbon. The highest number of differentially regulated transcripts was observed in thalli exposed to 2 °C plus high irradiance. Algal 13C isotopic discrimination values suggested an absence of down-regulation of carbon-concentrating mechanisms at high CO2. CO2 enrichment induced few transcriptomic changes. There was high and constitutive gene expression of many photochemical and inorganic carbon utilization components, which might be related to the strong adaptation of D. anceps to the Antarctic environment. These results suggest that increased temperature and CO2 will allow D. anceps to maintain its productivity while tolerating higher irradiances than at present conditions.

Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Benthos; Bicarbonate, standard deviation; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, total; Carbon, total, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carotenoids; Carotenoids, standard deviation; Chlorophyll a; Chlorophyll a, standard deviation; Chlorophyll c1+c2; Chlorophyll c1+c2, standard deviation; Chromista; Desmarestia anceps; Dissolved organic carbon release rate; Dissolved organic carbon release rate, standard deviation; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross photosynthesis rate, oxygen; Gross photosynthesis rate, standard deviation; Growth/Morphology; Growth rate; Growth rate, standard deviation; Inhibition of net photosynthesis; Inhibition of net photosynthesis, standard deviation; Irradiance; Irradiance, standard deviation; Laboratory experiment; Laboratory strains; Light; Light saturation, standard deviation; Macroalgae; Maximal electron transport rate; Maximal electron transport rate, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Net photosynthesis rate, oxygen; Net photosynthesis rate, standard deviation; Nitrogen, total; Nitrogen, total, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Percentage; Percentage, standard deviation; pH; pH, standard deviation; Photosynthetic efficiency; Photosynthetic efficiency, standard deviation; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Ratio; Ratio, standard deviation; Registration number of species; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Saturation light intensity; Single species; Species; Temperature; Temperature, water; Treatment; Type; Uniform resource locator/link to reference; δ13C; δ13C, standard deviation

Type: Dataset

Format: text/tab-separated-values, 624 data points

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

DATA PANGAEA

Fulltext

7

Unknown

Increased temperature and CO2 alleviate photoinhibition in Desmarestia anceps: from transcriptomics to carbon utilization (2017)

Iñiguez, Concepción ; Heinrich, Sandra ; Harms, Lars ; [et al.]

OXFORD UNIV PRESS

In: EPIC3Journal of Experimental Botany, OXFORD UNIV PRESS, ISSN: 0022-0957

add to mindlist on the mindlist

Details

Publication Date: 2017-06-03

Description: Ocean acidification and warming are affecting polar regions with particular intensity. Rocky shores of the Antarctic Peninsula are dominated by canopy-forming Desmarestiales. This study investigates the physiological and transcriptomic responses of the endemic macroalga Desmarestia anceps to a combination of different levels of temperature (2 and 7 °C), dissolved CO2 (380 and 1000 ppm), and irradiance (65 and 145 µmol photons m−2 s−1). Growth and photosynthesis increased at high CO2 conditions, and strongly decreased at 2 °C plus high irradiance, in comparison to the other treatments. Photoinhibition at 2 °C plus high irradiance was evidenced by the photochemical performance and intensive release of dissolved organic carbon. The highest number of differentially regulated transcripts was observed in thalli exposed to 2 °C plus high irradiance. Algal 13C isotopic discrimination values suggested an absence of down-regulation of carbon-concentrating mechanisms at high CO2. CO2 enrichment induced few transcriptomic changes. There was high and constitutive gene expression of many photochemical and inorganic carbon utilization components, which might be related to the strong adaptation of D. anceps to the Antarctic environment. These results suggest that increased temperature and CO2 will allow D. anceps to maintain its productivity while tolerating higher irradiances than at present conditions.

Repository Name: EPIC Alfred Wegener Institut

Type: Article , isiRev

Format: application/pdf

Permalink