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  • 1
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Exposure of harvested grapefruit to ultraviolet (UV) light induced resistance against the green mould decay caused by Penicillium digitatum. Grapefruit picked at various times during the harvest season responded differently to UV treatments. The UV dose required for development of maximum resistance increased as the season progressed. The initial UV dose required in November-picked fruit for maximum response was 4·8 kJ/m2. It declined to 1·6 and 3·2 kJ/m2 in December- and January-picked fruit, respectively, and increased to 8 kJ/m2 in February-picked fruit. Correspondingly, the minimum percentage infection developing after UV treatment increased throughout the season from 0 to 35%. Resistance in UV-treated fruit developed to its maximum extent at 24–48 h following exposure to UV light and then decreased. Development of induced resistance in grapefruit peel was affected by the temperature at which the fruit was stored 24 h after UV treatment and before infection with P. digitatum. In the UV-treated fruit, the fungus developed a sporadic mycelium with marked inhibition of sporulation. The activity of phenylalanine ammonia-lyase and peroxidase markedly increased in the peel following exposure of the fruit to UV light.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Pesticide Biochemistry and Physiology 31 (1988), S. 1-12 
    ISSN: 0048-3575
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 14 (1995), S. 345-348 
    ISSN: 1432-203X
    Keywords: floating membrane raft ; liquid medium ; solid medium micropropagation ; Aconitum napellus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient method was developed using floating membrane rafts (Liferaft™) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 162 (1984), S. 422-426 
    ISSN: 1432-2048
    Keywords: Blue light photoreceptor ; Cryptochrome ; Cucumis ; Hypocotyl (elongation, blue light) ; Phytochrome (elongation growth)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An interaction is demonstrated between the effects of phytochrome and cryptochrome (the specific blue-light photoreceptor) in the inhibition of hypocotyl elongation of light-grown cucumber (Cucumis sativus L.) cv. Ridge Greenline seedlings. At certain fluence rates of blue light the total inhibition response is greater than the sum of the separate responses to each photoreceptor. The threshold for response to blue light is reduced at least 30-fold by additional red-light irradiation. The synergistic effect is demonstrated for two different fluence rates of red light. Synergism is mediated by phytochrome in both the cotyledons and the hypocotyl.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 152 (1981), S. 70-73 
    ISSN: 1432-2048
    Keywords: Cotyledons ; Cucumis ; Lipase ; Lipid degradation ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The degradation of neutral lipid and the development of lipase activity in cucumber cotyledons is stimulated by white light. Malate synthase and isocitrate lyase activities show no stimulation. Lipase activity and neutral lipid breakdown are also enhanced by red light, far-red light proving ineffective. Far-red light reverses the effect of red light indicating the involvement of phytochrome in the control of lipase activity. Although light stimulates neutral lipid degradation it appears that much of the additional lipid lost is used in the synthesis of polar lipid constituents. Furthermore, the influence of light on lipid degradation appears to be species dependent.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Planta 164 (1985), S. 264-271 
    ISSN: 1432-2048
    Keywords: Cucumis (phytochrome) ; End-of-day response (phytochrome) ; Growth (phytochrome) ; Hypocotyl (elongation) ; Light (hypocotyl growth) ; Phytochrome photostationary state
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The control by phytochrome of hypocotyl elongation of light-grown Cucumis sativus L. after a white-light period was examined. The farred-absorbing form of phytochrome inhibits hypocotyl elongation. The response to phytochrome photostationary state (ϕ) is not linear; all values of ϕ from 0.004 to 0.13 promote growth maximally, in the range of values of ϕ from 0.13 to 0.22 there is a linear growth response, between values of ϕ of 0.22 and 0.35 there is again no differential effect, and for ϕ values above 0.35 there is a strong (near linear) effect of ϕ on elongation. A kinetic examination of events following the white-light period shows that the major recovery from the photoperiod requires 8.5 h of darkness. End-of-day far-red treatment produces a very different response pattern, with a minor growth stimulation within 28 min of treatment followed by a major effect after 80 to 90 min. Three hours after far-red treatment there is a transient decline in growth rate which persists for about 2 h. Over the whole time course there is a great stimulation of growth rate compared with the controls. A similar growth-rate pattern also occurs if the end-of-day ϕ is 0.48, although the magnitude of the growth stimulation is less. Two components are affected by end-of-day ϕ, namely the time at which growth recovers and the subsequent growth rate. In the long term, the latter accounts for most of the differences in elongation growth. The dark recovery when only the hypocotyl is irradiated requires 4 h, but end-of-day far-red treatment reduces this to about 1.5 h. The persistence of the far-red-absorbing form of phytochrome for many hours in darkness in these light-grown plants is also demonstrated.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5044
    Keywords: cantaloupe ; Cucumis melo ; genetic transformation ; GUS ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A modified particle inflow gun (PIG), that utilized a plastic vacuum chamber, was compared with a conventional PIG by bombarding cantaloupe (Cucumis melo L.) cotyledon basal quarters with plasmid pBI221 (Clontech Inc.) containing a β-glucuronidase (GUS) gene adsorbed onto tungsten particles. Both guns produced an equivalent number of transient GUS foci when tested at 410 kPa (60 psi), 620 kPa (90 psi) or 830 kPa (120 psi) helium and at a 10, 15 or 20 cm gap between the specimen and DNA/particle holder screen. For both guns, treatments utilizing the lower pressure and/or the greater distance generally produced significantly fewer GUS-positive foci. The plastic PIG was convenient to operate and could be built with simple hand tools in less than 40 minutes, using readily available parts.
    Type of Medium: Electronic Resource
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