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  • 1
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    Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis (2015)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2015-03-15
    Description: Sepsis is a frequently fatal condition characterized by an uncontrolled and harmful host reaction to microbial infection. Despite the prevalence and severity of sepsis, we lack a fundamental grasp of its pathophysiology. Here we report that the cytokine interleukin-3 (IL-3) potentiates inflammation in sepsis. Using a mouse model of abdominal sepsis, we showed that innate response activator B cells produce IL-3, which induces myelopoiesis of Ly-6C(high) monocytes and neutrophils and fuels a cytokine storm. IL-3 deficiency protects mice against sepsis. In humans with sepsis, high plasma IL-3 levels are associated with high mortality even after adjusting for prognostic indicators. This study deepens our understanding of immune activation, identifies IL-3 as an orchestrator of emergency myelopoiesis, and reveals a new therapeutic target for treating sepsis.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4376966/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4376966/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Weber, Georg F -- Chousterman, Benjamin G -- He, Shun -- Fenn, Ashley M -- Nairz, Manfred -- Anzai, Atsushi -- Brenner, Thorsten -- Uhle, Florian -- Iwamoto, Yoshiko -- Robbins, Clinton S -- Noiret, Lorette -- Maier, Sarah L -- Zonnchen, Tina -- Rahbari, Nuh N -- Scholch, Sebastian -- Klotzsche-von Ameln, Anne -- Chavakis, Triantafyllos -- Weitz, Jurgen -- Hofer, Stefan -- Weigand, Markus A -- Nahrendorf, Matthias -- Weissleder, Ralph -- Swirski, Filip K -- 5R01HL095612/HL/NHLBI NIH HHS/ -- R01 HL095612/HL/NHLBI NIH HHS/ -- R56 AI104695/AI/NIAID NIH HHS/ -- R56-AI104695/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2015 Mar 13;347(6227):1260-5. doi: 10.1126/science.aaa4268.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. Department of Visceral, Thoracic and Vascular Surgery, Technische Universitat Dresden, Dresden, Germany. fswirski@mgh.harvard.edu georg.weber@uniklinikum-dresden.de. ; Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. ; Department of Anesthesiology, University of Heidelberg, Heidelberg, Germany. ; Department of Visceral, Thoracic and Vascular Surgery, Technische Universitat Dresden, Dresden, Germany. ; Department of Clinical Pathobiochemistry and Institute for Clinical Chemistry and Laboratory Medicine, Technische Universitat Dresden, Dresden, Germany. ; Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. ; Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. fswirski@mgh.harvard.edu georg.weber@uniklinikum-dresden.de.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25766237" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; B-Lymphocyte Subsets/immunology ; Cytokines/immunology/metabolism ; Disease Models, Animal ; Humans ; Inflammation ; Interleukin-3/blood/*immunology/metabolism ; Lipopolysaccharides/immunology ; Lymphoid Tissue/immunology ; Mice ; Mice, Inbred BALB C ; Monocytes/immunology ; Myelopoiesis ; Neutrophils/immunology ; Peritonitis/immunology/pathology ; Prognosis ; Sepsis/*immunology/mortality/pathology/therapy
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Extreme Features of the Galdieria sulphuraria Organellar Genomes: A Consequence of Polyextremophily? (2015)
    Oxford University Press
    Details
    Publication Date: 2015-02-02
    Description: Nuclear genome sequencing from extremophilic eukaryotes has revealed clues about the mechanisms of adaptation to extreme environments, but the functional consequences of extremophily on organellar genomes are unknown. To address this issue, we assembled the mitochondrial and plastid genomes from a polyextremophilic red alga, Galdieria sulphuraria strain 074 W, and performed a comparative genomic analysis with other red algae and more broadly across eukaryotes. The mitogenome is highly reduced in size and genetic content and exhibits the highest guanine–cytosine skew of any known genome and the fastest substitution rate among all red algae. The plastid genome contains a large number of intergenic stem-loop structures but is otherwise rather typical in size, structure, and content in comparison with other red algae. We suggest that these unique genomic modifications result not only from the harsh conditions in which Galdieria lives but also from its unusual capability to grow heterotrophically, endolithically, and in the dark. These conditions place additional mutational pressures on the mitogenome due to the increased reliance on the mitochondrion for energy production, whereas the decreased reliance on photosynthesis and the presence of numerous stem-loop structures may shield the plastome from similar genomic stress.
    Electronic ISSN: 1759-6653
    Topics: Biology
    Published by Oxford University Press
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  • 3
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    Nuclear osteopontin-c is a prognostic breast cancer marker (2015)
    Nature Publishing Group (NPG)
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    Publication Date: 2015-02-19
    Description: Nuclear osteopontin-c is a prognostic breast cancer marker British Journal of Cancer 112, 729 (17 February 2015). doi:10.1038/bjc.2014.664 Authors: K Zduniak, P Ziolkowski, C Ahlin, A Agrawal, S Agrawal, C Blomqvist, M-L Fjällskog & G F Weber
    Keywords: biomarkerimmunohistochemistrytissue arraybreast cancercancer progressionprognosis
    Print ISSN: 0007-0920
    Electronic ISSN: 1532-1827
    Topics: Medicine
    Published by Nature Publishing Group (NPG)
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  • 4
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    Innate response activator B cells protect against microbial sepsis (2012)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2012-01-17
    Description: Recognition and clearance of a bacterial infection are a fundamental properties of innate immunity. Here, we describe an effector B cell population that protects against microbial sepsis. Innate response activator (IRA) B cells are phenotypically and functionally distinct, develop and diverge from B1a B cells, depend on pattern-recognition receptors, and produce granulocyte-macrophage colony-stimulating factor. Specific deletion of IRA B cell activity impairs bacterial clearance, elicits a cytokine storm, and precipitates septic shock. These observations enrich our understanding of innate immunity, position IRA B cells as gatekeepers of bacterial infection, and identify new treatment avenues for infectious diseases.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279743/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279743/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rauch, Philipp J -- Chudnovskiy, Aleksey -- Robbins, Clinton S -- Weber, Georg F -- Etzrodt, Martin -- Hilgendorf, Ingo -- Tiglao, Elizabeth -- Figueiredo, Jose-Luiz -- Iwamoto, Yoshiko -- Theurl, Igor -- Gorbatov, Rostic -- Waring, Michael T -- Chicoine, Adam T -- Mouded, Majd -- Pittet, Mikael J -- Nahrendorf, Matthias -- Weissleder, Ralph -- Swirski, Filip K -- 1R01HL095612/HL/NHLBI NIH HHS/ -- P01-A154904/PHS HHS/ -- P50 CA086355/CA/NCI NIH HHS/ -- P50 CA086355-11/CA/NCI NIH HHS/ -- P50 CA86355/CA/NCI NIH HHS/ -- R01 HL095612/HL/NHLBI NIH HHS/ -- R01 HL095612-03/HL/NHLBI NIH HHS/ -- R24 CA69246/CA/NCI NIH HHS/ -- S10 RR026360/RR/NCRR NIH HHS/ -- U01 HL080731/HL/NHLBI NIH HHS/ -- U01 HL080731-04/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2012 Feb 3;335(6068):597-601. doi: 10.1126/science.1215173. Epub 2012 Jan 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22245738" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; B-Lymphocyte Subsets/*immunology/metabolism ; Cell Lineage ; Cell Separation ; Escherichia coli Infections/*immunology ; Female ; Flow Cytometry ; Granulocyte-Macrophage Colony-Stimulating Factor/immunology/*metabolism ; *Immunity, Innate ; Immunoglobulin M/metabolism ; Immunophenotyping ; Integrin alpha4beta1/immunology/metabolism ; Lipopolysaccharides ; Lymphocyte Activation ; Lymphocyte Function-Associated Antigen-1/immunology/metabolism ; Mice ; Mice, Inbred C57BL ; Parabiosis ; Peritonitis/*immunology ; Sepsis/*immunology ; Shock, Septic/immunology ; Spleen/immunology ; Toll-Like Receptor 4/immunology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 5
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    Mechanical stress-activated integrin {alpha}5{beta}1 induces opening of connexin 43 hemichannels [Cell Biology] (2012)
    National Academy of Sciences
    Details
    Publication Date: 2012-02-29
    Description: The connexin 43 (Cx43) hemichannel (HC) in the mechanosensory osteocytes is a major portal for the release of factors responsible for the anabolic effects of mechanical loading on bone formation and remodeling. However, little is known about how the Cx43 molecule responds to mechanical stimulation leading to the opening of the HC. Here, we demonstrate that integrin α5β1 interacts directly with Cx43 and that this interaction is required for mechanical stimulation-induced opening of the Cx43 HC. Direct mechanical perturbation via magnetic beads or conformational activation of integrin α5β1 leads to the opening of the Cx43 HC, and this role of the integrin is independent of its association with an extracellular fibronectin substrate. PI3K signaling is responsible for the shear stress-induced conformational activation of integrin α5β1 leading to the opening of the HC. These results identify an unconventional function of integrin that acts as a mechanical tether to induce opening of the HC and provide a mechanism connecting the effect of mechanical forces directly to anabolic function of the bone.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 6
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    Fra-2 regulates B cell development by enhancing IRF4 and Foxo1 transcription (2017)
    Rockefeller University Press
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    Publication Date: 2017-07-04
    Description: The role of AP-1 transcription factors in early B cell development and function is still incompletely characterized. Here we address the role of Fra-2 in B cell differentiation. Deletion of Fra-2 leads to impaired B cell proliferation in the bone marrow. In addition, IL-7–stimulated pro–B cell cultures revealed a reduced differentiation from large pre–B cells to small B cells and immature B cells. Gene profiling and chromatin immunoprecipitation sequencing analyses unraveled a transcriptional reduction of the transcription factors Foxo1 , Irf4 , Ikaros , and Aiolos in Fra-2–deficient B cells. Moreover, expression of IL7Rα and Rag 1/2 , downstream targets of Irf4 and Foxo1, were also reduced in the absence of Fra-2. Pro–B cell proliferation and small pre–B cell differentiation were fully rescued by expression of Foxo1 and Irf4 in Fra-2–deficient pro–B cells. Hence, Fra-2 is a key upstream regulator of Foxo1 and Irf4 expression and influences proliferation and differentiation of B cells at multiple stages.
    Print ISSN: 0022-1007
    Electronic ISSN: 1540-9538
    Topics: Medicine
    Published by Rockefeller University Press
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  • 7
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    Mechanical and signaling roles for keratin intermediate filaments in the assembly and morphogenesis of Xenopus mesendoderm tissue at gastrulation [RESEARCH ARTICLE] (2017)
    The Company of Biologists
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    Publication Date: 2017-11-29
    Description: Pooja R. Sonavane, Chong Wang, Bette Dzamba, Gregory F. Weber, Ammasi Periasamy, and Douglas W. DeSimone The coordination of individual cell behaviors is a crucial step in the assembly and morphogenesis of tissues. Xenopus mesendoderm cells migrate collectively along a fibronectin (FN) substrate at gastrulation, but how the adhesive and mechanical forces required for these movements are generated and transmitted is unclear. Traction force microscopy (TFM) was used to establish that traction stresses are limited primarily to leading edge cells in mesendoderm explants, and that these forces are balanced by intercellular stresses in follower rows. This is further reflected in the morphology of these cells, with broad lamellipodial protrusions, mature focal adhesions and a gradient of activated Rac1 evident at the leading edge, while small protrusions, rapid turnover of immature focal adhesions and lack of a Rac1 activity gradient characterize cells in following rows. Depletion of keratin (krt8) with antisense morpholinos results in high traction stresses in follower row cells, misdirected protrusions and the formation of actin stress fibers anchored in streak-like focal adhesions. We propose that maintenance of mechanical integrity in the mesendoderm by keratin intermediate filaments is required to balance stresses within the tissue to regulate collective cell movements.
    Print ISSN: 0950-1991
    Electronic ISSN: 1477-9129
    Topics: Biology
    Published by The Company of Biologists
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  • 8
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    Innate Response Activator B Cells Aggravate Atherosclerosis by Stimulating T Helper-1 Adaptive Immunity [Vascular Medicine] (2014)
    American Heart Association (AHA)
    Details
    Publication Date: 2014-04-23
    Description: Background— Atherosclerotic lesions grow via the accumulation of leukocytes and oxidized lipoproteins in the vessel wall. Leukocytes can attenuate or augment atherosclerosis through the release of cytokines, chemokines, and other mediators. Deciphering how leukocytes develop, oppose, and complement each other’s function and shape the course of disease can illuminate our understanding of atherosclerosis. Innate response activator (IRA) B cells are a recently described population of granulocyte macrophage colony-stimulating factor–secreting cells of hitherto unknown function in atherosclerosis. Methods and Results— Here, we show that IRA B cells arise during atherosclerosis in mice and humans. In response to a high-cholesterol diet, IRA B cell numbers increase preferentially in secondary lymphoid organs via Myd88-dependent signaling. Mixed chimeric mice lacking B cell–derived granulocyte macrophage colony-stimulating factor develop smaller lesions with fewer macrophages and effector T cells. Mechanistically, IRA B cells promote the expansion of classic dendritic cells, which then generate interferon –producing T helper-1 cells. This IRA B cell–dependent T helper-1 skewing manifests in an IgG1-to-IgG2c isotype switch in the immunoglobulin response against oxidized lipoproteins. Conclusions— Granulocyte macrophage colony-stimulating factor–producing IRA B cells alter adaptive immune processes and shift the leukocyte response toward a T helper-1–associated milieu that aggravates atherosclerosis.
    Keywords: Cell biology/structural biology, Growth factors/cytokines, Mechanism of atherosclerosis/growth factors
    Electronic ISSN: 1524-4539
    Topics: Medicine
    Published by American Heart Association (AHA)
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  • 9
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    The amino acid metabolite homocysteine activates mTORC1 to inhibit autophagy and form abnormal proteins in human neurons and mice [Research] (2017)
    The Federation of American Societies for Experimental Biology (FASEB)
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    Publication Date: 2017-02-02
    Description: The molecular mechanisms leading to and responsible for age-related, sporadic Alzheimer’s disease (AD) remain largely unknown. It is well documented that aging patients with elevated levels of the amino acid metabolite homocysteine (Hcy) are at high risk of developing AD. We investigated the impact of Hcy on molecular clearance pathways in mammalian cells, including in vitro cultured induced pluripotent stem cell-derived forebrain neurons and in vivo neurons in mouse brains. Exposure to Hcy resulted in up-regulation of the mechanistic target of rapamycin complex 1 (mTORC1) activity, one of the major kinases in cells that is tightly linked to anabolic and catabolic pathways. Hcy is sensed by a constitutive protein complex composed of leucyl-tRNA-synthetase and folliculin, which regulates mTOR tethering to lysosomal membranes. In hyperhomocysteinemic human cells and cystathionine β-synthase-deficient mouse brains, we find an acute and chronic inhibition of the molecular clearance of protein products resulting in a buildup of abnormal proteins, including β-amyloid and phospho-Tau. Formation of these protein aggregates leads to AD-like neurodegeneration. This pathology can be prevented by inhibition of mTORC1 or by induction of autophagy. We conclude that an increase of intracellular Hcy levels predisposes neurons to develop abnormal protein aggregates, which are hallmarks of AD and its associated onset and pathophysiology with age.—Khayati, K., Antikainen, H., Bonder, E. M., Weber, G. F., Kruger, W. D., Jakubowski, H., Dobrowolski, R. The amino acid metabolite homocysteine activates mTORC1 to inhibit autophagy and form abnormal proteins in human neurons and mice.
    Print ISSN: 0892-6638
    Electronic ISSN: 1530-6860
    Topics: Biology
    Published by The Federation of American Societies for Experimental Biology (FASEB)
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  • 10
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    Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis (2014)
    Rockefeller University Press
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    Publication Date: 2014-06-03
    Description: Pneumonia is a major cause of mortality worldwide and a serious problem in critical care medicine, but the immunophysiological processes that confer either protection or morbidity are not completely understood. We show that in response to lung infection, B1a B cells migrate from the pleural space to the lung parenchyma to secrete polyreactive emergency immunoglobulin M (IgM). The process requires innate response activator (IRA) B cells, a transitional B1a-derived inflammatory subset which controls IgM production via autocrine granulocyte/macrophage colony-stimulating factor (GM-CSF) signaling. The strategic location of these cells, coupled with the capacity to produce GM-CSF–dependent IgM, ensures effective early frontline defense against bacteria invading the lungs. The study describes a previously unrecognized GM-CSF-IgM axis and positions IRA B cells as orchestrators of protective IgM immunity.
    Print ISSN: 0022-1007
    Electronic ISSN: 1540-9538
    Topics: Medicine
    Published by Rockefeller University Press
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