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Spinal cord injury induces expression of RGS7 in microglia/macrophages in rats (2002)

Hausmann, Oliver N. ; Hu, Wen-Hui ; Keren-Raifman, Tal ; [et al.]

Oxford, UK : Blackwell Science, Ltd

European journal of neuroscience 15 (2002), S. 0

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ISSN: 1460-9568

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: RGS proteins regulate G protein-mediated signalling pathways through direct interaction with the Gα subunits and facilitation of GTP hydrolysis. An RGS subfamily consisting of RGS 6, 7, 9, and 11 also interacts with the G protein β subunit Gβ5 via a characteristic Gγ-like domain. Thus far, these complexes were found only in neurons, with RGS7 being the most widely distributed in the brain. Here we confirm the expression of RGS7 in spinal neurons and show as a novel finding that following an experimental spinal cord injury in rats, expression of RGS7 is induced in a subpopulation of other cells. Immunofluorescent confocal microscopy using a series of cell specific antibodies identified these RGS7 positive cells as activated microglia and/or invading peripheral macrophages. To rule out interference from the adjacent neurons and confirm the presence of RGS7-Gβ5 complex in inflammatory cells, we performed immunocytochemistry, RT-PCR, Western blot, and immunoprecipitation using microglial (BV2) and peripheral macrophage (RAW) cell lines. Expression of RGS7 mRNA and protein are nearly undetectable in non-stimulated BV2 and RAW cells, but remarkably increased after stimulation with LPS or TNF-α In addition, RGS7-positive cells were also found in the perinodular rim in the rat spleen. Our findings show that RGS7-Gβ5 complex is expressed in immunocompetent cells such as resident microglia and peripheral macrophages following spinal cord injury. This expression might contribute to the post-traumatic inflammatory responses in the central nervous system.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1460-9568.2002.01916.x

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Recent progress in immunoisolated cell therapy (1994)

Lysaght, Michael J. ; Frydel, Beata ; Gentile, Frank ; [et al.]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 56 (1994), S. 196-203

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ISSN: 0730-2312

Keywords: immuniosmlation therapy ; transplantation ; cell therapy ; therapeutic gene prducts ; encapsulation ; diabetes ; neurodegenerative discorders ; Parkinson's ; Alzheimers ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: Biohybrid implalts represent a new class of medical device in which living cells, supported in a hydrogel matrix, and surrounded by semipepmiable membrane, produce and deliver therapeutic reagents tm specific sites wthin a host. First prpmsed in the mid-1970s for tpeatment modality has progressed rapidly in the past four years and is now being investigated not just for endcrine disorders but also for alleviation of chronic pain, treatment of neurodegenerative disorders, and delivery of neurotrophic factors to sites withil the blood brain barrier, and aq a practical alternative to conventional ex vivo.

Additional Material: 4 Ill.