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  • 1
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    Perspectives of renewable energy in the Danube region (2015)
    Pécs : Hungarian Acad. of Sciences
    Details
    Publication Date: 2019-04-01
    Keywords: ddc:600
    Repository Name: Wuppertal Institut für Klima, Umwelt, Energie
    Language: English
    Type: conferenceobject , doc-type:conferenceObject
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    PAPER (OA)
  • 2
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of Citrus limon 26S rRNA gene and secondary structure model of its RNA (1990)
    Springer
    Plant molecular biology 14 (1990), S. 147-161 
    Details
    ISSN: 1573-5028
    Keywords: Citrus limon ; expansion segments ; rDNA sequencing ; 26S rRNA ; secondary structure model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The complete nucleotide sequence of Citrus limon 26S rDNA has been determined. The sequence has been aligned with large ribosomal RNA (L-rRNA) sequences of Escherichia coli, Saccharomyces cerevisiae and Oryza sativa. Nine extensive expansion segments in dicot 26S rRNA relative to E. coli 23S rRNA have been identified and compared with analogous segments of monocot, yeast, amphibian and human L-rRNAs. A secondary structure model for lemon 26S rRNA has been derived based on the refined model of E. coli 23S rRNA. It has been compared with other eukaryotic L-rRNAs models in terms of location of functionally important regions. Origin and evolution of L-rRNA expansion segments are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018556
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic selection of lambda phage clones from a gene clonotheque (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 312-315 
    Details
    ISSN: 1617-4623
    Keywords: Homologous recombination ; Transduction ; Phagemid ; α-interferon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A genetic procedure for selection of specific λ clones, by homologous recombination between λ clones from a gene clonotheque and sequences cloned into a plasmid, was developed. Resulting clones are isolated in transduction experiments by plating infected Escherichia coli cells under conditions selecting for the antibiotic resistance marker carried by the plasmid. The feasibility of the method was demonstrated in a model test system as well as by isolation of α-interferon-specific sequences from the human gene clonotheque.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333972
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Analysis of the Equalization of Inverted Repeats and Neurovirulence Using a Pseudorabies Virus Mutant Strain Altered at the Ul/Ir Junction (1998)
    Springer
    Virus genes 17 (1998), S. 89-98 
    Details
    ISSN: 1572-994X
    Keywords: herpesvirus ; pseudorabies (Aujeszky’s disease) virus ; equalization ; neurovirulence determinant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A recombinant pseudorabies (Aujeszky’s disease) virus (PrV) designated as vE16lac was constructed by deleting a 3-kbp DNA segment spanning the junction of long and short components of the viral genome, and by replacing the deleted segment with a lacZ-expression cassette. The aim of constructing this mutant was (a) to determine whether the terminal repeat (Tr) can serve as a template for the regeneration of the internal repeat (Ir), and (b) whether this deletion causes a reduction in the neuroinvasiveness of the virus. To analyze the mechanism of equalization, revertant viruses were selected and structurally characterized from vE16lac infection of PK-15 cells, mice and pigs. Because all revertants acquired Ir sequences identical to that of the wild-type virus, the equalization process occurred using the Tr as a template to reconstitute the Ir. We also found that the recombinant virus vE16lac was virulent in both pigs and mice. The data are discussed in view of studies performed with similar PrV mutants by other authors (Rall et al., 1992, Dean and Cheung, 1995 and Dean et al., 1996).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008061220442
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Overexpression and purification of enzymatically active recombinant integrase protein of rous sarcoma virus (1992)
    Springer
    Virus genes 6 (1992), S. 301-306 
    Details
    ISSN: 1572-994X
    Keywords: molecular cloning ; gene expression ; integrase protein ; enzyme purification ; Rous sarcoma virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The carboxy-terminal domain of polymerase gene of Rous sarcoma virus was cloned into an expression vector under the control oflac regulatory elements, resulting in the plasmid pMF1413. Upon isopropyl-β-D-thiogalactopyranoside induction, viral integration (IN) protein was expressed in large quantity inEscherichia coli. The expressed recombinant protein was prepurified by successive washing of the bacterial pellet with 0.1 M NaCl and detergents. Further purification was performed in high yield by standard chromatography methods. The purified enzyme revealed selective DNA cleaving activity on supercoiled plasmid with the LTR-LTR junction fragment. The reaction was metal ion dependent, with a preference for Mn2+ over Mg2+, and showed substrate specificity at 1 mM MnCl2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01702568
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Transient expression assay in a baculovirus system using firefly luciferase gene as a reporter (1992)
    Springer
    Virus genes 6 (1992), S. 379-386 
    Details
    ISSN: 1572-994X
    Keywords: recombinant DNA ; gene expression ; polyhedrin gene promoter ; expression vector ; DNA sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Transient gene expression assays were developed to assess the function of the regulatory sequences of baculovirusesBombyx mori nuclear polyhedrosis virus (BmNPV) andAutographa californica nuclear polyhedrosis virus (AcNPV) in insect cells ofBombyx mori andSpodoptera frugiperda, respectively. DNA sequences encoding luciferase (luc) of the fireflyPhotinus pyralis was successfully employed in the expression assay as a reporter gene. Recombinant plasmids were constructed containing the luc gene under control of baculovirus-specific or heterologous promoters. Cotransfection ofBombyx mori andSpodoptera frugiperda cells with recombinant plasmids carrying virus-specific promoter sequences and BmNPV and AcNPV DNA, respectively, gave rise to efficient synthesis of luciferase (Luc), while heterologous promoters induced a low level of luc expression. We found that flanking sequences of the AcNPV DNA in the transfer plasmid contained an unknown promoter conferring an efficient luc expression. The activity of this promoter was modulated by the polh promoter sequences. The assay allows one to conduct highly sensitive monitoring of the transient expression of foreign genes from the transfecting plasmids prior to construction of recombinant viruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01703086
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    Paper (German National Licenses)
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  • 7
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    A multi-system geochronology in the Ad-3 borehole, Pannonian Basin (Hungary) with implications for dating volcanic rocks by low-temperature thermochronology and for interpretation of (U-Th)/He data (2015)
    Wiley-Blackwell
    Details
    Publication Date: 2015-04-24
    Description: Independent geochronological and thermal modelling approaches are applied to a biostratigraphically exceptionally well-controlled borehole, Alcsútdoboz Ad-3, in order to constrain the age of Cenozoic geodynamic events in the western Pannonian Basin and to test the efficacy of the methods for dating volcanic rocks. Apatite fission track and zircon U-Pb data show two volcanic phases of Middle Eocene (43.4-39.0 Ma) and Early Oligocene (32.72±0.15 Ma) age, respectively. Apatite (U-Th)/He ages (23.8-14.8 Ma) and independent thermal and subsidence history models reveal a brief period of heating to 55-70°C at ~17 Ma caused by an increased heat-flow related to crustal thinning and mantle upwelling. Our results demonstrate that, contrary to common perception, the apatite (U-Th)/He method is likely to record ‘apparent’ or ‘mixed’ ages resulting from subsequent thermal events rather than ‘cooling’ or ‘eruption’ ages directly related to distinct geological events. It follows that a direct conversion of ‘apparent’ or ‘mixed’ (U-Th)/He ages into cooling, exhumation or erosion rates is incorrect. This article is protected by copyright. All rights reserved.
    Print ISSN: 0954-4879
    Electronic ISSN: 1365-3121
    Topics: Geosciences
    Published by Wiley-Blackwell
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