ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
: The potential use of the non-pathogenic ciliate Tetrahymena thermophila for converting cholesterol into pro-vitamin D sterols in egg suspensions was investigated. Conditions screened for efficient conversion included inoculum size and age, egg and egg yolk dilution, initial pH, and aeration. By optimizing these parameters, 55%± 6 cholesterol reduction was obtained in 24 hours incubation. Tetrahymena converted cholesterol into three unsaturated sterols, D7,22 bis-dehydrocholesterol, an ergosterol analog (27%), D7-dehydrocholesterol, also known as pro-vitamin D3 (4%), and Δ22- dehydrocholesterol (8%). Despite significant proteolysis, culturing with Tetrahymena did not result in net consumption of total amino acids content, which was in fact increased by 16%± 2. These findings emphasize the potential use of Tetrahymena in the food industry.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2002.tb09561.x
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