ISSN:
1573-5028
Keywords:
Agrobacterium tumefaciens
;
T-DNA
;
plant transformation
;
Ac/Ds transposable elements
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Different patterns of T-DNA integration in Arabidopsis were obtained that depended on whether a root or a leaf-disc transformation method was used. An examination of 82 individual transgenic Arabidopsis plants, derived from 15 independent Agrobacterium-mediated transformations in which different cointegrate and binary constructs were used, indicated that the transformation method had a significant influence on the type and copy number of T-DNA integration events. Southern hybridizations showed that most of the transgenic plants produced by a leaf-disc method contained multiple T-DNA insertions (89%), the majority of which were organized as right-border inverted repeat structures (58%). In contrast, a root transformation method mostly resulted in single T-DNA insertions (64%), with fewer right-border inverted repeats (38%). The transformation vectors, including cointegrate and binary types, and the plant selectable markers, hygromycin phosphotransferase and dihydrofolate reductase, did not appear to influence the T-DNA integration patterns.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00021539
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