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  • 1
    Publication Date: 2020-06-19
    Description: The dichotomy between high microbial abundance (HMA) and low microbial abundance (LMA) sponges has been long recognized. In the present study, 56 sponge species from three geographic regions (greater Caribbean, Mediterranean, Red Sea) were investigated by transmission electron microscopy for the presence of microorganisms in the mesohyl matrix. Additionally, bacterial enumeration by DAPI-counting was performed on a subset of samples. Of the 56 species investigated, 28 were identified as belonging to the HMA and 28 to the LMA category. The sponge orders Agelasida and Verongida consisted exclusively of HMA species, and the Poecilosclerida were composed only of LMA sponges. Other taxa contained both types of microbial associations (e.g., marine Haplosclerida, Homoscleromorpha, Dictyoceratida), and a clear phylogenetic pattern could not be identified. For a few sponge species, an intermediate microbial load was determined, and the microscopy data did not suffice to reliably determine HMA or LMA status. To experimentally determine the HMA or LMA status of a sponge species, we therefore recommend a combination of transmission electron microscopy and 16S rRNA gene sequence data. This study significantly expands previous reports on microbial abundances in sponge tissues and contributes to a better understanding of the HMA-LMA dichotomy in sponge-microbe symbioses.
    Type: Article , PeerReviewed
    Format: text
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  • 2
    Publication Date: 2018-11-09
    Type: Conference or Workshop Item , NonPeerReviewed
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  • 3
    Publication Date: 2014-11-07
    Description: Comparison of Sollasella digitata Lendenfeld, 1888, up until the present assigned to its own family Sollasellidae Lendenfeld, 1887 in the order Hadromerida, and Raspailopsis cervicornis Burton, 1959, assigned to Raspailiidae Nardo, 1833 in the order Poecilosclerida, leads to the conclusion that both should be considered congeneric and are best assigned to a single genus Sollasella. This conclusion is based on examination of habit and skeletal characters of the type material of S. digitata and both type and freshly collected material of S. cervicornis. The conclusion is strengthened by the discovery of a new species, Sollasella moretonensis n.sp. collected in North Australia (primarily in the northeastern coast, but also an isolated record from the northwestern Australian coast), which possesses in addition to the characteristic surface pattern and skeletal structure, genuine echinating acanthostyles. The redefined genus Sollasella shares axial / extra-axial arrangement of the skeleton, special surface brushes of oxeas surrounding a single protruding style, and vestigial occurrence of acanthostyles with many Raspailia s.l. Nevertheless, it is retained as a separate genus, on account of its peculiar polygonal arrangement of surface pores. The distribution of the genus is disjunctive including both (southeast, northeast and northwest) Australian and Western Indian Ocean localities, but so far no intermediate records. Based on this morphological evidence, it is proposed – pending publication of corroborating molecular evidence to be presented in a follow-up study – to reassign Sollasella and the family Sollasellidae to the poecilosclerid family Raspailiidae.
    Keywords: sponges ; classification ; Sollasellidae ; Raspailiidae ; Sollasella ; Raspailopsis ; Australia ; Oman ; 42.72
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
    Format: application/pdf
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  • 4
    Publication Date: 2024-01-12
    Description: Comparison of Sollasella digitata Lendenfeld, 1888, up until the present assigned to its own family Sollasellidae Lendenfeld, 1887 in the order Hadromerida, and Raspailopsis cervicornis Burton, 1959, assigned to Raspailiidae Nardo, 1833 in the order Poecilosclerida, leads to the conclusion that both should be considered congeneric and are best assigned to a single genus Sollasella. This conclusion is based on examination of habit and skeletal characters of the type material of S. digitata and both type and freshly collected material of S. cervicornis. The conclusion is strengthened by the discovery of a new species, Sollasella moretonensis n.sp. collected in North Australia (primarily in the northeastern coast, but also an isolated record from the northwestern Australian coast), which possesses in addition to the characteristic surface pattern and skeletal structure, genuine echinating acanthostyles. The redefined genus Sollasella shares axial / extra-axial arrangement of the skeleton, special surface brushes of oxeas surrounding a single protruding style, and vestigial occurrence of acanthostyles with many Raspailia s.l. Nevertheless, it is retained as a separate genus, on account of its peculiar polygonal arrangement of surface pores. The distribution of the genus is disjunctive including both (southeast, northeast and northwest) Australian and Western Indian Ocean localities, but so far no intermediate records. Based on this morphological evidence, it is proposed \xe2\x80\x93 pending publication of corroborating molecular evidence to be presented in a follow-up study \xe2\x80\x93 to reassign Sollasella and the family Sollasellidae to the poecilosclerid family Raspailiidae.
    Keywords: sponges ; classification ; Sollasellidae ; Raspailiidae ; Sollasella ; Raspailopsis ; Australia ; Oman
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 5
    Publication Date: 2024-01-12
    Description: This is the second part of a revision and re-classification of the demosponge family Sollasellidae, and an example of a successful use of combined morphological and molecular data. Sollasella had been a poorly known, long forgotten taxon, placed incertae sedis in the order Hadromerida in the last major revision of the demosponges. It has recently been suggested to belong to Raspailiidae in the order Poecilosclerida due to striking morphological similarities. The present analysis verified this re-classification using molecular markers. Comparing 28S rDNA fragments of Sollasella cervicornis, a newly described species S. moretonensis and a representative set of raspailiid and hadromerid samples. In our analyses Sollasella clearly clusters inside the Raspailiidae clade, and distantly from hadromerid taxa. Supporting morphological hypothesis of Van Soest et al. (2006), that Sollasella is a raspailiid sponge.
    Keywords: sponges ; classification ; Raspailiidae ; Sollasella ; Raspailopsis ; 28S rDNA ; molecular systematics
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 6
    Publication Date: 2024-01-12
    Description: The giant barrel sponge Xestospongia testudinaria is an ecologically important species that is widely distributed across the Indo-Pacific. Little is known, however, about the precise biogeographic distribution and the amount of morphological and genetic variation in this species. Here we provide the first detailed, fine-scaled (〈200 km2) study of the morphological and genetic composition of X. testudinaria around Lembeh Island, Indonesia. Two mitochondrial (CO1 and ATP6 genes) and one nuclear (ATP synthase \xce\xb2 intron) DNA markers were used to assess genetic variation. We identified four distinct morphotypes of X. testudinaria around Lembeh Island. These morphotypes were genetically differentiated with both mitochondrial and nuclear markers. Our results indicate that giant barrel sponges around Lembeh Island, which were all morphologically identified as X. testudinaria, consist of at least two different lineages that appear to be reproductively isolated. The first lineage is represented by individuals with a digitate surface area, CO1 haplotype C5, and is most abundant around the harbor area of Bitung city. The second lineage is represented by individuals with a predominantly smooth surface area, CO1 haplotype C1 and can be found all around Lembeh Island, though to a lesser extent around the harbor of Bitung city. Our findings of two additional unique genetic lineages suggests the presence of an even broader species complex possibly containing more than two reproductively isolated species. The existence of X. testudinaria as a species complex is a surprising result given the size, abundance and conspicuousness of the sponge.
    Keywords: Xestospongia testudinaria ; Indo-Pacific ; DNA ; genetic composition ; sponge
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 7
    Publication Date: 2024-01-12
    Description: The existence of multiple independently derived populations in landlocked marine lakes provides an opportunity for fundamental research into the role of isolation in population divergence and speciation in marine taxa. Marine lakes are landlocked water bodies that maintain a marine character through narrow submarine connections to the sea and could be regarded as the marine equivalents of terrestrial islands. The sponge Suberites diversicolor (Porifera: Demospongiae: Suberitidae) is typical of marine lake habitats in the Indo-Australian Archipelago. Four molecular markers (two mitochondrial and two nuclear) were employed to study genetic structure of populations within and between marine lakes in Indonesia and three coastal locations in Indonesia, Singapore and Australia. Within populations of S. diversicolor two strongly divergent lineages (A & B) (COI: p = 0.4% and ITS: p = 7.3%) were found, that may constitute cryptic species. Lineage A only occurred in Kakaban lake (East Kalimantan), while lineage B was present in all sampled populations. Within lineage B, we found low levels of genetic diversity in lakes, though there was spatial genetic population structuring. The Australian population is genetically differentiated from the Indonesian populations. Within Indonesia we did not record an East-West barrier, which has frequently been reported for other marine invertebrates. Kakaban lake is the largest and most isolated marine lake in Indonesia and contains the highest genetic diversity with genetic variants not observed elsewhere. Kakaban lake may be an area where multiple putative refugia populations have come into secondary contact, resulting in high levels of genetic diversity and a high number of endemic species.
    Keywords: Suberites diversicolor ; Indo-Australian Archipelago ; marine lakes ; evolution
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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