GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    The Type III Secretion System Effector SeoC of Salmonella enterica subsp. salamae and S. enterica subsp. arizonae ADP-Ribosylates Src and Inhibits Opsonophagocytosis [Cellular Microbiology: Pathogen-Host Cell Molecular Interactions] (2016)
    The American Society for Microbiology (ASM)
    Details
    Publication Date: 2016-11-19
    Description: Salmonella species utilize type III secretion systems (T3SSs) to translocate effectors into the cytosol of mammalian host cells, subverting cell signaling and facilitating the onset of gastroenteritis. In this study, we compared a draft genome assembly of Salmonella enterica subsp. salamae strain 3588/07 against the genomes of S. enterica subsp. enterica serovar Typhimurium strain LT2 and Salmonella bongori strain 12419. S. enterica subsp. salamae encodes the Salmonella pathogenicity island 1 (SPI-1), SPI-2, and the locus of enterocyte effacement (LEE) T3SSs. Though several key S . Typhimurium effector genes are missing (e.g., avrA , sopB , and sseL ), S. enterica subsp. salamae invades HeLa cells and contains homologues of S. bongori sboK and sboC , which we named seoC . SboC and SeoC are homologues of EspJ from enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), which inhibit Src kinase-dependent phagocytosis by ADP-ribosylation. By screening 73 clinical and environmental Salmonella isolates, we identified EspJ homologues in S. bongori , S. enterica subsp. salamae , and Salmonella enterica subsp. arizonae . The β-lactamase TEM-1 reporter system showed that SeoC is translocated by the SPI-1 T3SS. All the Salmonella SeoC/SboC homologues ADP-ribosylate Src E310 in vitro . Ectopic expression of SeoC/SboC inhibited phagocytosis of IgG-opsonized beads into Cos-7 cells stably expressing green fluorescent protein (GFP)-FcRIIa. Concurrently, S. enterica subsp. salamae infection of J774.A1 macrophages inhibited phagocytosis of beads, in a seoC -dependent manner. These results show that S. bongori , S. enterica subsp. salamae , and S. enterica subsp. arizonae share features of the infection strategy of extracellular pathogens EPEC and EHEC and shed light on the complexities of the T3SS effector repertoires of Enterobacteriaceae .
    Print ISSN: 0019-9567
    Electronic ISSN: 1098-5522
    Topics: Medicine
    Published by The American Society for Microbiology (ASM)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Interregional foodborne salmonellosis outbreak due to powdered infant formula contaminated with lactose-fermenting Salmonella virchow (1996)
    Springer
    European journal of epidemiology 12 (1996), S. 377-381 
    Details
    ISSN: 1573-7284
    Keywords: Foodborne-outbreak ; Powdered infant formula ; Salmonella virchow lactose +
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Spain's Salmonella surveillance system backed by regionally-based epidemiologists around the country made it possible to detect and halt the spread of a foodborne salmonellosis outbreak due to powdered infant formula contaminated with a lactose-fermenting strain of Salmonella virchow. Forty-eight cases involving children, mostly under 7 months old, were detected in 14 out of Spain's 17 Regions. The outbreak started in January and ended in June 1994. All cases were microbiologically confirmed. The implicated strain had a 3.6 kb plasmid, was susceptible to all antimicrobials tested except nitrofurantoin and was phagetype 4a. Isolates from 8 of 24 Brand “A” milk samples tested had the same characteristics as case isolates. All affected/suspect batches of Brand “A” milk were destroyed and the product withdrawn from sale, which led to the end of the outbreak. This incident underscores the importance of maintaining surveillance systems able to detect and prevent foodborne outbreaks and alert to the possibility of isolating unusual lactose-fermenting Salmonella serotypes in especially sensitive food products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00145301
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Investigation of an outbreak of Salmonella typhi in a public school in Madrid (1993)
    Springer
    European journal of epidemiology 9 (1993), S. 251-254 
    Details
    ISSN: 1573-7284
    Keywords: Typhoid fever ; Epidemiology ; Salmonella typhi ; Plasmid analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A typhoid fever outbreak affecting 54 school students occurred in a Public School of Móstoles, Madrid. The date of onset was 11 June 1991 and the last detected case was 8 July 1991. Salmonella typhi was cultured from blood and/or stool samples corresponding to 54 patients and one food-handler. There were no secondary cases detected. Epidemiological investigation suggested a salad or a custard as the common source. Patients and the food-handler were treated with ampicillin/amoxicillin for up to three weeks. There were seven relapses that were also treated with the same antibiotics with success. None were found to be excreting the organisms when tested after four months. All the Salmonella ryphi isolated strains were phagetype 34, biotype Xylose +, Tetrationate Reductase + and harboured a similar 22 Mdal plasmid, they were also susceptible to the antibiotics tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00146259
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Molecular subtyping ofVibrio cholerae O1 strains recently isolated from patient, food and environmental samples in Spain (1994)
    Springer
    European journal of clinical microbiology & infectious diseases 13 (1994), S. 299-303 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract NineteenVibrio cholerae O1 strains isolated in Spain from patient, food and environmental samples in the period 1990–1992 were characterized by detection of cholera toxin by enzyme immunoassay, detection of cholera toxin gene by polymerase chain reaction, and by biotyping, ribotyping and pulsed-field gel electrophoresis. Ten isolates were toxigenic and were further characterized by multilocus enzyme electrophoresis. Molecular subtyping methods allowed precise differentiation between isolates, indicating their geographic origin. Isolates associated with the ongoing seventh pandemic were distinguishable from those associated with the present Latin American epidemic. All isolates from the environment and seafood were nontoxigenic, and were genetically different and more diverse than toxigenic isolates. The data suggest that a focus of endemic cholera does not exist in Spain, and that the analyzed nontoxigenicVibrio cholerae O1 isolates from imported seafood were not a threat to public health.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974604
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Multiple analysis of a foodborne outbreak caused by infant formula contaminated by an atypicalSalmonella virchow strain (1998)
    Springer
    European journal of clinical microbiology & infectious diseases 17 (1998), S. 551-555 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An outbreak of food poisoning involving most autonomous Spanish communities was detected in the first half of 1994. The causative food was infant formula milk contaminated by lactose-fermentingSalmonella virchow. It was not possible to isolate the causative strain from the manufacturer's facilities. During the same period of time, there was a significant increase in lactose-non-fermentingSalmonella virchow strains compared with the same period in previous years. Simultaneously, lactose-non-fermenting strains were recovered from clinical samples from children and from some milk samples that were involved in the outbreak. Therefore, it was speculated that the outbreak might be more extensive than initially thought. The following epidemiological markers were used for typing theSalmonella virchow strains involved in the outbreak: (i) phage typing; (ii) ribotyping, using a set of 20 different endonucleases; and (iii) pulsed-field gel electrophoresis, using three different endonucleases. The most useful markers for this serotype were phage typing and pulsed-field gel electrophoresis, since ribotyping was not able to distinguish all strains tested. The results obtained revealed that the outbreak was caused by at least two strains: one presenting phage type 4-4a and pulsed-field patterns Al or A2 and L + or L−, and another presenting phage type 2 and pulsed-field patterns A1 or A2 and L + or L−. The results indicate that the outbreak was more extensive than initially thought and that the Virchow serotype is very clonal in Spain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01708617
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of Phenotypic and Genotypic Markers for Characterisation of the Emerging Gastroenteritis Pathogen Salmonella hadar (2000)
    Springer
    European journal of clinical microbiology & infectious diseases 19 (2000), S. 275-281 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Over the last 4 years Salmonella hadar has increasingly been isolated in Europe in conjunction with food-borne gastroenteritis. The aim of this study was to evaluate epidemiological methods (phage typing, antimicrobial susceptibility testing, DNA plasmid analysis, ribotyping and pulsed-field gel electrophoresis analysis) for characterising Salmonella hadar isolates. The 100% phage typeability of isolates and the high discriminatory index of 0.8856 suggest that phage typing is the method of choice. In order to obtain subdivisions of the most frequent Salmonella hadar phage types, a combination of molecular methods, such as ribotyping performed with BglI and EcoRI or pulsed-field gel electrophoresis using XbaI and XhoI, would be desirable as the usefulness of each technique varies with the phage type being analysed. Of note was the high (86%) rate of resistance to tetracycline and nalidixic acid but full susceptibility to ciprofloxacin in the strains studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100960050475
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Multiple Analysis of a Foodborne Outbreak caused by Infant Formula Contaminated by an Atypical Salmonella virchow Strain (1998)
    Springer
    European journal of clinical microbiology & infectious diseases 17 (1998), S. 551-555 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  An outbreak of food poisoning involving most autonomous Spanish communities was detected in the first half of 1994. The causative food was infant formula milk contaminated by lactose-fermenting Salmonella virchow. It was not possible to isolate the causative strain from the manufacturer's facilities.During the same period of time, there was a significant increase in lactose-non-fermenting Salmonella virchow strains compared with the same period in previous years. Simultaneously, lactose-non-fermenting strains were recovered from clinical samples from children and from some milk samples that were involved in the outbreak. Therefore, it was speculated that the outbreak might be more extensive than initially thought. The following epidemiological markers were used for typing the Salmonella virchow strains involved in the outbreak: (i) phage typing; (ii) ribotyping, using a set of 20 different endonucleases; and (iii) pulsed-field gel electrophoresis, using three different endonucleases. The most useful markers for this serotype were phage typing and pulsed-field gel electrophoresis, since ribotyping was not able to distinguish all strains tested. The results obtained revealed that the outbreak was caused by at least two strains: one presenting phage type 4–4a and pulsed-field patterns Al or A2 and L+ or L–, and another presenting phage type 2 and pulsed-field patterns Al or A2 and L+ or L–. The results indicate that the outbreak was more extensive than initially thought and that the Virchow serotype is very clonal in Spain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01708617
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Occurrence ofSalmonella in cold-blooded animals in Gran Canaria, Canary Islands, Spain (1995)
    Springer
    Antonie van Leeuwenhoek 68 (1995), S. 191-194 
    Details
    ISSN: 1572-9699
    Keywords: Salmonella ; cold-blooded animals ; Gran Canaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence ofSalmonella in endemic and subendemic species of lizard and frog of Gran Canaria,Gallotia stehlini andRana perezi, as well as captive reptiles from other regions of the world was investigated. The occurrence ofSalmonella was statistically higher in endemic and subendemic species than in captive animals (p〈0.001). Seventy strains ofSalmonella were isolated.S. berta andS. grancanaria were the most frequently isolated serotypes. The study ofSalmonella in gall-bladder contents showed a high parasitation (85%), being higher inGallotia stehlini (100%) than inRana perezi (60%). None of the isolated salmonellae were resistant to tested antibiotics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00871814
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...