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  • 1
    Electronic Resource
    Electronic Resource
    Somaclonal variation in cell suspension culture-derived regenerants of Cymbopogon martinii (Roxb.) Wats var. motia (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Plants regenerated from cell suspension cultures of palmarosa grass, Cymbopogon martinii (Roxb.) Wats, were analysed for somaclonal variation in five clonal generations. A wide range of variation in important quantitative traits, e.g. plant yield, height, tiller number, oil content and qualitative changes in essential oil constituents—geraniol, geranyl acetate, geranyl formate and linalool—were observed among the 120 somaclones screened. Eight somaclones were selected on the basis of high herb and oil yield over the donor line and high geraniol content in the oil. Based on performance in the field trials, three superior lines were selected, and maintained for five clonal generations. The superior lines exhibited a reasonable degree of stability in the traits selected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.1999.00383.x
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  • 2
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martinii) (1997)
    Springer
    Plant cell reports 16 (1997), S. 430-434 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cell suspension culture ; Cymbopogon martinii ; Monocot ; Palmarosa ; Plant regeneration ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell suspension culture was established from nodal callus of Cymbopogon martinii (Roxb.) Wats in a liquid medium containing Murashige and Skoog (1962) basal salts, vitamins, 100 mg l–1 myo-inositol and 20 g l–1 of sucrose (MS) that was supplemented with 13.6 µM 2,4-dichlorophenoxyacetic acid and 1.15 µM kinetin. An initial inoculum density of 2 × 104 cells ml–1 exhibited optimum cell growth. Calli were obtained 12–15 days after the suspension was plated onto semisolid medium of a similar composition. When calli were transferred to semisolid regeneration medium containing MS + 6.7 µM N 6-benzyl-adenine + 1.15 µM kinetin, somatic embryogenesis and plantlet regeneration occurred after 10–25 days. There was no significant decrease in the regeneration potential of the calli even when the cultures were initiated from 47-week-old cell suspensions. Chromosome counts of cells in suspensions, calli and somatic embryos derived from cultures of different ages revealed the presence of diploids, tetraploids and octaploids. However, the 33 regenerated plants tested were all diploid, indicating that only diploid cells were capable of regeneration in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146788
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  • 3
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martinii) (1997)
    Springer
    Plant cell reports 16 (1997), S. 430-434 
    Details
    ISSN: 1432-203X
    Keywords: Cell suspension culture ; Cymbopogon martinii ; Monocot ; Palmarosa ; Plant regeneration ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell suspension culture was established from nodal callus ofCymbopogon martinii (Roxb.) Wats in a liquid medium containing Murashige and Skoog (1962) basal salts, vitamins, 100 mg 1−1 myo-inositol and 20 g l−1 of sucrose (MS) that was supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid and 1.15 μM kinetin. An initial inoculum density of 2 x 104 cells ml−1exhibited optimum cell growth. Calli were obtained 12–15 days after the suspension was plated onto semisolid medium of a similar composition. When calli were transferred to semisolid regeneration medium containing MS + 6.7 μM N 6-benzyl-adenine + 1.15 μM kinetin, somatic embryogenesis and plantlet regeneration occurred after 10–25 days. There was no significant decrease in the regeneration potential of the calli even when the cultures were initiated from 47-week-old cell suspensions. Chromosome counts of cells in suspensions, calli and somatic embryos derived from cultures of different ages revealed the presence of diploids, tetraploids and octaploids. However, the 33 regenerated plants tested were all diploid, indicating that only diploid cells were capable of regeneration in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146788
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  • 4
    Electronic Resource
    Electronic Resource
    Rapid propagation of lemongrass (Cymbopogon flexuosus (Nees) Wats.) through somatic embryogenesis in vitro (1996)
    Springer
    Plant cell reports 15 (1996), S. 367-370 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryos induced from callus cultures of lemongrass [Cymbopogon flexuosus (Nees) Wats.] on Murashige and Skoog medium supplemented with 5 mg/l of 2,4-D, 0.1 mg/l of NAA and 0.5 mg/l of Kn developed into plantlets when plated on a medium supplemented with 3 mg/l of BA, 1 mg/l of GA3 and 0.1 mg/l of NAA. The regeneration potential of callus was retained for more than 2 years on the nutrient medium supplemented with comparatively lower levels of growth regulators (2,4-D at 2 mg/l, NAA at 0.1 mg/l and Kn at 0.25 mg/l). Approximately 30–35 plantlets were produced after two months of culture per 100 mg of callus inoculated. Regenerants were transplanted into soil and transferred to the field for assessment of various morphological and biochemical characteristics. The results of 1 year of field trials showed that plants derived from somatic embryoids were more uniform in all the characteristics examined when compared with the field performance of plants raised through slips by standard propagation procedures. Thus, a procedure has been developed for high frequency long term plant production of lemongrass through in vitro methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232373
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  • 5
    Electronic Resource
    Electronic Resource
    Micropropagation of Hemidesmus indicus (L.) R. Br. through axillary bud culture (1996)
    Springer
    Plant cell reports 15 (1996), S. 427-430 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure for rapid in vitro propagation of the aromatic and medicinal plant Hemidesmus indicus (L.) R.Br. (Family Asclepiadaceae) from nodal explants is described. The highest shoot multiplication rate of 8.2 ± 0.4 shoots/explant with a 95% frequency was achieved in S weeks culture period on Murashige and Skoog medium supplemented with 1.15 μM kinetin and 0.054 μM α-naphthaleneacetic acid. Excised shoots were rooted on the same basal medium supplemented with 1.15 μM kinetin and 7.35 μM indole-3-butyric acid. Shoots derived from subcultures exhibited better rooting response than those from primary cultures. After a hardening phase of 2 weeks, there was a 70% transplantation success in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232069
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  • 6
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis in callus cultures of Rosa hybrida L. cv. Landora (1991)
    Springer
    Plant cell, tissue and organ culture 27 (1991), S. 65-69 
    Details
    ISSN: 1573-5044
    Keywords: callus ; in vitro ; L-proline ; Rosa hybrida cv. Landora ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus was initiated from immature leaf and stem segments of rose (Rosa hybrida cv. Landora) and subcultured every four weeks on a basal medium of half-strength Murashige & Skoog (1962) salts plus 30 g l-1 sucrose (1/2 MS) and supplemented with 2.2 μM BA, 5.4 μM NAA and 2.2–9.0 μM 2,4-D. Embryogenic callus and subsequently somatic embryos were obtained from 8-week-old callus culture on 1/2 MS+2.2 μM BA+0.05 μM NAA+0.3 μM GA3+200−800 mg l-1 L-proline. Long-term cultures were established and maintained for up to 16 months by repeated subculture of embryogenic callus on L-proline deficient medium. About 12% of cotyledonary stage embryos taken from cultures cold-stored at 8±1°C for 4 days germinated on 1/2 MS+2.2 μM BA+0.3 μM GA3+24.7 μM adenine sulphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048208
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