Keywords:
Botanical chemistry.
;
Electronic books.
Description / Table of Contents:
The Biochemistry of Plants, Volume 13: Methodology focuses on the biological applications of filter paper chromatography. This book explores the developments in the technology of countercurrent liquid chromatography that led to the emergence of machines involving droplet chromatography, centrifugal chromatography, and planet coil centrifugal chromatography. Organized into six chapters, this volume starts with an overview of the methods of enzymology and the immunochemical techniques that enable biochemists to elucidate cellular processes that are not readily investigated by other techniques. This book then emphasizes the use of the specific antigen-antibody reaction to localize antigens in tissue sections. Other chapters consider the rationale underlying the use of mutants to study plant biochemistry. This text discusses as well the practical aspects of nuclear magnetic resonance (NMR) spectroscopy, which can generate various data about chemically complex mixtures, such as living cells. Biochemists, organic chemists, and biologists will find this book extremely useful.
Type of Medium:
Online Resource
Pages:
1 online resource (309 pages)
Edition:
1st ed.
ISBN:
9780323162470
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=1181877
DDC:
581
Language:
English
Note:
Front Cover -- Methodology -- Copyright Page -- Table of Contents -- List of Contributors -- General Preface -- Preface to Volume 13 -- Chapter 1. Immunochemistry for Enzymology -- I. INTRODUCTION -- II. PRODUCTION OF ANTIBODIES -- III. IMMUNOCHEMICAL STUDIES OF PROTEINS -- IV. APPLICATION OF IMMUNOCHEMISTRY TO STUDIES OF ENZYME INDUCTION AND DEGRADATION -- V. IMMUNOCHEMICAL STUDIES OF LOW-MOLECULAR-WEIGHT METABOLITES -- VI. CONCLUSIONS -- REFERENCES -- Chapter 2. The Use of Mutants for the Study of Plant Metabolism -- I. INTRODUCTION -- II. ANALYSIS OF METABOLIC PATHWAYS -- III. ANALYSIS OF REGULATORY MECHANISMS -- IV. NEW TECHNIQUES -- V. CONCLUDING REMARKS -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 3. The Use of Plant Cell Cultures in Studies of Metabolism -- I. INTRODUCTION -- II. ISOLATION AND CHARACTERIZATION OF VARIANT CELL LINES IN CULTURE -- III. METABOLISM OF AMINO ACIDS -- IV. METABOLISM OF AMINO ACID-DERIVED SECONDARY PRODUCTS -- V· CONCLUSIONS -- REFERENCES -- Chapter 4. The Application of Mass Spectrometry to Biochemical and Physiological Studies -- I. INTRODUCTION -- II. ISOTOPIC DISCRIMINATION -- III. STUDIES WITH 1 80 (WITH ASIDES ON OTHER ISOTOPES OF O) -- IV. STUDIES WITH 2H (D) WITH A BRIEF COMPARISON WITH 3H (T) -- V. STUDIES WITH 15N (WITH COMMENTS ON 13N, C2H2, 36ClO3 …, AND 14CH3NH3+) -- VI. STUDIES WITH 13C (WITH COMMENTS ON 11C AND 14C) -- VII. CONCLUSIONS -- REFERENCES -- Chapter 5. NMR in Plant Biochemistry -- I. INTRODUCTION -- II. THEORY OF FOURIER-TRANSFORM NMR -- III. MEASUREMENTS POSSIBLE WITH NMR SPECTROSCOPY -- IV. PRACTICAL ASPECTS OF IN VIVO NMR -- V. IN VIVO 31P-NMR SPECTROSCOPY OF PLANTS -- VI. STUDIES OF ION TRANSPORT AND METABOLISM -- VII. STUDIES OF METABOLIC PATHWAYS -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 6. Electron Spin Resonance -- I. INTRODUCTION TO THE ESR METHOD.
,
II. NATURALLY OCCURRING PARAMAGNETIC CENTERS -- III. APPLICATIONS TO PLANT METABOLISM -- IV. CONCLUDING REMARKS -- V. GLOSSARY -- REFERENCES -- Index -- Contents of Other Volumes.
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