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  • 1
    Online Resource
    Online Resource
    New York, NY :Springer,
    Keywords: Physical measurements-Congresses. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (647 pages)
    Edition: 1st ed.
    ISBN: 9781489921451
    Series Statement: NATO Science Series B: Series ; v.98
    Language: English
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  • 2
    Online Resource
    Online Resource
    Totowa :Humana Press,
    Keywords: Proteins -- Purification -- Laboratory manuals. ; Biomolecules. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (498 pages)
    Edition: 2nd ed.
    ISBN: 9781592596553
    DDC: 547.7/5046
    Language: English
    Note: Intro -- Protein Purification Protocols: Second Edition -- Preface -- Contents -- Contributors -- 1 General Strategies -- 2 Preparation of Extracts From Animal Tissues -- 3 Protein Extraction From Plant Tissues -- 4 Extraction of Recombinant Protein From Bacteria -- 5 Protein Extraction From Fungi -- 6 Subecllular Fractionation of Animal Tissues -- 7 Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria -- 8 The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds -- 9 Avoidance of Proteolysis in Extracts -- 10 Concentration of Extracts -- 11 Making and Changing Buffers -- 12 Purification and Concentration by Ultrafiltration -- 13 Bulk Purification by Fractional Precipitation -- 14 Ion-Exchange Chromatography -- 15 Hydrophobic Interaction Chromatography -- 16 Affinity Chromatography -- 17 Dye-Ligand Affinity Chromatography -- 18 Lectin Affinity Chromatography -- 19 Immunoaffinity Chromatography -- 20 Immobilized Metal-Ion Affinity Chromatography -- 21 Chromatography on Hydroxyapatite -- 22 Thiophilic Affinity Chromatography and Related Methods -- 23 Affinity Precipitation Methods -- 24 Isoelectric Focusing -- 25 Chromatofocusing -- 26 Size-Exclusion Chromatography -- 27 Fast Protein Liquid Chromatography -- 28 Reversed-Phase Chromatography of Proteins -- 29 Extraction of Membrane Proteins -- 30 Removal of Detergent From Protein Fractions -- 31 Purification of Membrane Proteins -- 32 Lyophilization of Proteins -- 33 Storage of Pure Proteins -- 34 Electroelution of Proteins From Polyacrylamide Gels -- 35 Electroblotting of Proteins From Polyacrylamide Gels -- 36 Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses -- 37 Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for Comprehensive Proteome Analysis -- 38 Practical Column Chromatography. , 39 Detection Methods -- 40 Peptide Proteomics -- 41 Multidimensional Liquid Chromatography of Proteins -- 42 Mass Spectrometry -- 43 Purification of Therapeutic Proteins -- 44 Purification Process Scale-Up -- Index.
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  • 3
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 65 (1994), S. 2151-2153 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The geometry of an atomically sharp or nearly atomically sharp tip in proximity to a planar anode may be closely approximated in the prolate-spheroidal coordinate system. An exact three-dimensional electrostatic-potential solution for a free charge in such a tip/base junction is given in this letter, including calculations for both the symmetrical on-axis case and the asymmetric off-axis case. An exact solution for the potential-energy barrier is also given; this solution has immediate applications in three-dimensional tunneling studies and in calculations of electron trajectories in micron- and submicron-sized field-emitter arrays. © 1994 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 74 (1999), S. 1093-1095 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Recent studies of field emission from wide-band-gap semiconductors have concentrated on thin-film and needle geometries. It has been proposed that the emission originates from localized asperities (or crystallites) on the film (which can be of nanometer or even atomic size) or from very sharp tips approaching atomic size in the case of needle geometry. A quantity important in determining the origin of the tunneling electron states is the density of states function. In the present work we have calculated the local density of states at an atomically sharp diamond asperity (or tip) using a tight-binding model. A pyramidal-shaped cluster of 159 atoms is constructed to model the tip. The forces are calculated and used to optimize the atomic geometry of the top six layers of atoms. The bottom layers are fixed to simulate the bulk diamond. Results indicate that the local density of states of the topmost single atom on the tip is significantly different from that of the bulk and suggest that the discrete geometry of the structure plays a role in determining the field-emission characteristics. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 67 (1995), S. 3697-3701 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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