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  • 1
    Electronic Resource
    Electronic Resource
    Localization of arginine decarboxylase in tobacco plants (2004)
    Oxford, UK; Malden , USA : Munksgaard International Publishers
    Physiologia plantarum 120 (2004), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The lack of knowledge about the tissue and subcellular distribution of polyamines (PAs) and the enzymes involved in their metabolism remains one of the main obstacles in our understanding of the biological role of PAs in plants. Arginine decarboxylase (ADC; EC 4.1.1.9) is a key enzyme in polyamine biosynthesis in plants. We have characterized a cDNA coding for ADC from Nicotiana tabacum L. cv. Petit Havana SR1. The deduced ADC polypeptide had 721 amino acids and a molecular mass of 77 kDa. The ADC cDNA was overexpressed in Escherichia coli, and the ADC fusion protein obtained was used to produce polyclonal antibodies. Using immunological methods, we demonstrate the presence of the ADC protein in all plant organs analysed: flowers, seeds, stems, leaves and roots. Moreover, depending on the tissue, the protein is localized in two different subcellular compartments, the nucleus and the chloroplast. In photosynthetic tissues, ADC is located mainly in chloroplasts, whereas in non-photosynthetic tissues the protein appears to be located in nuclei. The different compartmentation of ADC may be related to distinct functions of the protein in different cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0031-9317.2004.0216.x
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance (1997)
    Springer
    Planta 201 (1997), S. 293-297 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Drought tolerance ; Nicotiana ; Osmotic stress ; Transgenic plants ; Trehalose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The yeast trehalose-6-phosphate synthase gene (TPS1) was engineered under the control of the cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants. Using Agrobacterium-mediated transfer, the gene was incorporated into the genomic DNA and constitutively expressed in Nicotiana tabacum␣L. plants. Trehalose was determined in the transformants, by anion-exchange chromatography coupled to pulsed amperometric detection. The non-reducing disaccharide accumulated up to 0.17 mg per g fresh weight in leaf extracts of transgenic plants. Trehalose-accumulating plants exhibited multiple phenotypic alterations, including stunted growth, lancet-shaped leaves, reduced sucrose content and improved drought tolerance. These pleiotropic effects, and the fact that water loss from detached leaves was not significantly affected by trehalose accumulation, suggest that synthesis of this sugar, rather than leading to an osmoprotectant effect, had altered sugar metabolism and regulatory pathways affecting plant development and stress tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050069
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  • 3
    Electronic Resource
    Electronic Resource
    Right-border sequences enable the left border of an Agrobacterium tumefaciens nopaline Ti-plasmid to produce single-stranded DNA (1988)
    Springer
    Plant molecular biology 11 (1988), S. 389-399 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; DNA transfer ; overdrive ; T-region ; virD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The T-region of nopaline-type Ti-plasmids (the portion of the plasmid that is transferred to plant cells) of Agrobacterium tumefaciens is delimited by 23–25 bp direct repeats. They are nicked by the products of the virD locus and the presence of these nicked sites is correlated with the synthesis of single-stranded T-region copies. Despite previous indications to the contrary, we show that the pTiT37 T-region left border is capable of producing single-stranded DNA with high efficiency and that its ability to do so is totally dependent on right border-proximal cis-acting sequences, most probably overdrive, located several kilobases from the border. The absence of overdrive does not affect the single-strand nicking activity of the virD product but only the production of single-stranded copies from the nicked substrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039019
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of p-dimethylaminoazobenzene and doxorubicin on tobacco cell growth (Nicotiana tabacum L.) (1987)
    Springer
    Plant growth regulation 5 (1987), S. 155-164 
    Details
    ISSN: 1573-5087
    Keywords: Tissue culture ; cell growth ; non-histone chromatin proteins ; Nicotiana ; p-dimethylaminoazobenzene ; doxorubicin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Callus growth, in tobacco pith tissue culture, is activated by p-dimethylaminoazobenzene (p-DAAB), whose carcinogenic properties are well known. Ultrastructural changes, appearing in a period prior to initiation of cell proliferation, occur earlier and more intensely in the presence of the carcinogen. This also influences changes in non-histone chromatin proteins (NHCP). Doxorubicin (DR), an anti-tumor drug, inhibits callus growth and modifies the pattern of NHCP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024742
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization, subcellular localization and nuclear targeting of casein kinase 2 from Zea mays (1999)
    Springer
    Plant molecular biology 40 (1999), S. 199-211 
    Details
    ISSN: 1573-5028
    Keywords: casein kinase 2 ; gene structure ; phosphorylation ; nuclear localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) α subunit using the previously described αCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100–150 bp. The sequence of the exons is 100% homologous to the sequence of the αCK2-1 cDNA. Southern hybridization of total genomic DNA from maize embryos with αCK2 cDNA indicated that the αCK2-1 gene is part of a multigenic family. We also isolated a new embryo cDNA clone coding for an αCK2-2 subunit. We studied the regulation of the enzyme in embryos at the mRNA level, at the protein level and by activity testing. By using immunocytochemistry the CK2 protein was localized in several types of cells of mature embryos. Particularly strong signals were visible in the cytoplasm of epidermis and meristematic cells. Decoration of nuclei of root cortex and scutellum cells was also observed suggesting that CK2 can shift from the cytoplasm into nuclei in specific cell types. We examined whether CK2 contained specific protein domains which actively target the protein to the nucleus by using in-frame fusions of the maize CK2 α subunit to the reporter gene encoding β-glucuronidase (GUS) which were assayed in transiently transformed onion epidermal cells. Analysis of chimeric constructs identified one region containing a nuclear localization signal (NLS) that is highly conserved in other αCK2 proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006196530079
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  • 6
    Electronic Resource
    Electronic Resource
    Expression and cellular localization of Atrab28 during Arabidopsis embryogenesis (1999)
    Springer
    Plant molecular biology 40 (1999), S. 355-363 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; embryo-specific gene expression ; Lea proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maize abscisic acid (ABA)-responsive gene rab28 has been shown to be ABA-inducible in embryos and vegetative tissues, expression being mostly restricted to vascular elements during late embryogenesis. In the course of an expressed sequence tags (ESTs) programme, we have isolated an Arabidopsis thaliana gene, Atrab28, encoding the orthologue of maize rab28. The Atrab28 cDNA is 1090 bp long, including a poly(A)+ stretch, and encodes a polypeptide of 262 amino acids. Atrab28 antibody against the recombinant protein recognizes a polipeptide of about 30 kDa and pI 6, in close agreement with the predicted molecular mass and pI. As for maize rab28, expression studies with Atrab28 revealed high specificity for embryo tissues, transcription being stimulated by the transcriptional activator abi3. In contrast, Atrab28 was not induced in vegetative tissues by ABA, osmotic stress or dehydration. The expression of Atrab28 mRNA and the accumulation of Atrab28 protein was largely restricted to provascular tissues of mature embryos and in the seed coat outer tegument and embryo and silique epidermis, as revealed by in situ hybridization and immunocytochemistry with anti-Atrab28 antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006219315562
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  • 7
    Electronic Resource
    Electronic Resource
    The expression of a peroxiredoxin antioxidant gene, AtPer1, in Arabidopsis thaliana is seed-specific and related to dormancy (1998)
    Springer
    Plant molecular biology 36 (1998), S. 833-845 
    Details
    ISSN: 1573-5028
    Keywords: embryo- and aleurone-specific expression ; desiccation tolerance ; thiol-specific antioxidant (TSA) ; late embryogenesis-abundant (lea) ; aba-1 and abi3-1 mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated a gene, AtPer1, from the dicotyledon Arabidopsis thaliana, which shows similarity to the 1-cysteine (1-Cys) peroxiredoxin family of antioxidants. In higher plants, members of this group of antioxidants have previously only been isolated from monocotyledons. It has been suggested that seed peroxiredoxins protect tissues from reactive oxygen species during desiccation and early imbibition and/or are involved in the maintenance of/protection during dormancy. AtPer1 expression is restricted to seeds. Despite differences in seed development between monocots and dicots, AtPer1 shows an expression pattern during seed development and germination similar to the dormancy-related transcript Per1 in barley. In situ hybridization identifies AtPer1 as the first aleurone-expressed transcript characterized in developing Arabidopsis seeds. The transcript is also expressed in the embryo. AtPer1 expression in seeds is unaltered in an ABA-deficient mutant (aba-1) during seed development, while expression in seeds of an ABA-insensitive mutant (abi3-1) is reduced. The transcript is not induced in vegetative tissue in response to stress by ABA or drought. AtPer1 transcript levels are correlated to germination frequencies of wildtype seeds, but AtPer1 transcript abundance is not sufficient for expression of dormancy in non-dormant mutants. Hypotheses on peroxiredoxin function are discussed in view of the results presented here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005900832440
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