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  • 1
    Electronic Resource
    Electronic Resource
    The Bardeen–Cooper–Schrieffer approach to electron correlation in the density matrix formalism (1997)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 107 (1997), S. 180-187 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: A generalization of the reduced density matrix theory when the number of particles is not fixed is given here. The first and second order reduced density matrices are defined with respect to a Bardeen–Cooper–Schrieffer ground state for molecules with an even number of electrons. This formalism is extended to molecules with an odd number of electrons. Valatin's generalized Hartree-Fock method is applied to the functional of energy. A new one-particle operator, V(circumflex)q, is thus obtained. This operator has two new terms. It takes into account all the correlation effects mainly through a new non-local potential L(circumflex)q — the Exchange and Time Inversion Potential. The operator V(circumflex)q thus correlates the motion of two particles with anti-parallel spins and determines a new set of spin-orbitals. The new set of equations is established and solved by using the MO-LCAO approximation. The method was tested for several molecules with good results. The calculated vertical ionization potentials compare satisfactorily with the experimental data. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.474363
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  • 2
    Electronic Resource
    Electronic Resource
    Production in Escherichia coli of a rat chimeric proinsulin polypeptide carrying human A and B chains and its preparative chromatography
    Amsterdam : Elsevier
    Journal of Biotechnology 38 (1994), S. 89-96 
    Details
    ISSN: 0168-1656
    Keywords: Expression vector ; Fusion protein ; Gene construction ; HPLC ; Inclusion body
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0168-1656(94)90150-3
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  • 3
    Electronic Resource
    Electronic Resource
    Recombinant protein production in cultures of an Escherichia coli trp − strain (1993)
    Springer
    Applied microbiology and biotechnology 39 (1993), S. 541-546 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Fermentation conditions were developed in order to achieve simultaneously a high biomass concentration and high-level expression of a hybrid cI-human insulin B peptide gene. In our system, this hybrid gene is under control of the Escherichia coli trp promoter, in a trp − derivative strain of E. coli W3110. The dual role of tryptophan concentration on cellular growth and hybrid gene regulation was studied in 10-l batch fermentations. In the best batch conditions, a biomass concentration of 12 g dry weight/l can be obtained, and 0.53 g/l of cI-insulin B hybrid protein is produced. Tryptophan in the culture medium is consumed by the growing culture, until a level is reached that causes induction of the hybrid gene. Plasmid loss was detected, as only 62% of the cells retained the recombinant plasmid. In order to increase the hybrid protein production level, a fed-batch culture strategy was developed whereby the specific growth rate of the cells was restrained. Using the same amount of nutrients as in the batch fermentations, it was possible to increase the final biomass concentration to 20 g/l, plasmid-bearing cells in the population to 90% and recombinant hybrid protein to 1.21 g/l.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00205048
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  • 4
    Electronic Resource
    Electronic Resource
    Vulnerability assessment of Angat water reservoir to climate change (1996)
    Springer
    Water, air & soil pollution 92 (1996), S. 191-201 
    Details
    ISSN: 1573-2932
    Keywords: Philippines ; water resources ; CCCM ; GFDL ; UKMO ; WATBAL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Global warming due to an anticipated doubling of carbon dioxide concentration in the atmosphere is expected to alter the earth's climate system within the next century. The potential changes in the climate system could affect hydrological cycles and processes. Possible impacts of climate change on water resources should be assessed to evaluate probable adaptation measures. In the Philippines, a preliminary assessment of the vulnerability of water resources to climate change and variability was undertaken. For this particular study, the Angat Reservoir was chosen as the study area. Because of its socioeconomic importance, it is useful to assess its vulnerability to climate change. A rainfall-runoff simulation model, WATBAL, was used to determine the effect of temperature and rainfall changes, based on CO2 doubling, on inflow to the reservoir. Climate change scenarios developed from results from three general circulation models and incremental changes were used. The results showed that changes in temperature and rainfall could affect runoff either positively or negatively. Using the temperature and rainfall changes from the Geophysical Fluid Dynamics Laboratory model there was a 32% increase in runoff, and with the Canadian Climate Centre Model, there was a 15% decrease in runoff. Under a climate scenario generated by the United Kingdom Meteorological Office model, runoff is estimated to increase by 5%. The use of incremental scenarios revealed the strong sensitivity of runoff to changes in rainfall as compared with changes in temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00175565
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  • 5
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    Addition of three species of the genus Plesionika (Crustacea: Caridea: Pandalidae) to the known Atlantic marine fauna of Colombia (2004)
    In:  Zoologische Mededelingen (00240672) vol.78, 1-16 (2004) p.131
    Details
    Publication Date: 2007-01-18
    Description: In recent sampling along the Colombian Caribbean coast, three species of the genus Plesionika (P. gigliolii, P. longicauda, and P. willesi) have been added to the seven reported upon by Campos et al. (in press). P. gigliolii is now recorded for the first time from the West Atlantic. The taxonomic status of P. acanthonotus is discussed. A key to the Atlantic species of Plesionika is provided.
    Keywords: Caridea ; Pandalidae ; Plesionika ; range extensions ; West Atlantic ; Colombia ; key ; 42.74
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
    Format: application/pdf
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  • 6
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    Nuevos registros de cangrejos del género Palicus (Philippi, 1838) (Crustacea: Decapoda: Brachyura: Palicidae) para el mar Caribe colombiano (2007)
    Details
    Publication Date: 2021-05-19
    Description: A new range extension of Palicus floridanus and five new records of Palicidae are presented, the samples are collected from the Colombian Caribbean coast during the cruise of INVEMAR-Macrofauna I between 1998 and 1999, along the upper continental Slope (300 and 500 m deep). Six species have now been recorded from the area: P. alternatus Rathbun, 1897; P. cursor A. Milne- Edwards, 1880; P. floridanus Rathbun, 1918; P. gracilipes A. Milne-Edwards, 1880; P. gracilis Smith, 1883, and P. sicus A. Milne-Edwards, 1880. Previously, P. alternatus was the only member of the family reported for this area before this study.
    Description: Published
    Description: Palicidae, Crabs
    Keywords: New records ; Marine crustaceans
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: pp.81-100
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  • 7
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    Nuevos registros de crustáceos brachyura y ampliación de la distribución de algunas especies para el mar Caribe colombiano. (2010)
    INVEMAR, Santa Marta (Colombia)
    Details
    Publication Date: 2021-05-19
    Description: Se registran por primera vez para el mar Caribe colombiano tres nuevas especies de cangrejos Brachyura: Tyche emarginata White, 1847, Piloslambrus guerini (Brito Capello, 1871) y Hepatus scaber Holthuis, 1959; y se amplía el ámbito de distribución hacia el norte del país para 20 especies previamente registradas, con ejemplares colectados durante el crucero INVEMAR-CORPOGUAJIRA en marzo y abril de 2005 en la plataforma continental (10 y 50 m de profundidad) de La Guajira.
    Description: Three species of Brachyura crabs, Tyche emarginata White, 1847; Piloslambrus guerini (Brito Capello, 1871), and Hepatus scaber Holthuis, 1959, are recorded for first time in Colombian Caribbean Sea. Also, the distribution range of 20 previously recorded species is widened towards the north of the country. Samples were collected during INVEMAR-CORPOGUAJIRA cruise between March and April 2005 at the continental shelf (10 and 50 m depth) in La Guajira.
    Description: Published
    Description: Crustaceans, Brachyura
    Keywords: Geographical distribution ; New records ; Geographical distribution ; Crab culture
    Repository Name: AquaDocs
    Type: Journal Contribution
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  • 8
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    Los camarones de la familia crangonidae de la franja superior del talud continental del mar Caribe colombiano (2009)
    INVEMAR - Santa Marta, Colombia
    Details
    Publication Date: 2021-05-19
    Description: Four surveys were conducted between October 1998 and April 1999 along the upper section of the Colombian Caribbean Sea continental slope with the aim of compliting marine fauna inventories. During the surveys (INVEMAR-MACROFAUNA 1) individuals of the shrimps Parapontocaris caribbaea, P. vicina, Prionocrangon pectinata and Parapontophilus gracilis (Decapoda: Crangonidae) were found and are herein registered for the first time in Colombian waters. Along with the description of the collected material and reference to site of collection, identification keys, diagnostic characters, diagrams and bathimetric and geographic distribution are included as well as comments on morphological variations of the individuals.
    Description: Entre octubre de 1998 y abril de 1999 se realizaron cuatro cruceros de exploración a lo largo de la franja superior del talud continental del mar Caribe colombiano con el fin de complementar los inventarios de la fauna marina. Durante esta expedición (INVEMAR - MACROFAUNA I) se colectaron ejemplares de cuatro especies de camarones de la familia Crangonidae: Parapontocaris caribbaea, P. vicina, Prionocrangon pectinata y Parapontophilus gracilis las cuales se registran por primera vez en aguas colombianas. Se incluyen claves de identificación, caracteres diagnósticos de las especies, descripciones con base en el material colectado, imágenes y datos de distribución batimétrica y geográfica. También se presentan comentarios acerca de las variaciones morfológicas encontradas en los especímenes.
    Description: Published
    Description: Parapontocaris; Parapontophilus; Prionocrangon
    Keywords: Continental slope
    Repository Name: AquaDocs
    Type: Journal Contribution
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  • 9
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    Addition of three species of the genus Plesionika (Crustacea: Caridea: Pandalidae) to the known Atlantic marine fauna of Colombia (2004)
    In:  Zoologische Mededelingen vol. 78, 1-16, pp. 131-146
    Details
    Publication Date: 2024-01-12
    Description: In recent sampling along the Colombian Caribbean coast, three species of the genus Plesionika (P. gigliolii, P. longicauda, and P. willesi) have been added to the seven reported upon by Campos et al. (in press). P. gigliolii is now recorded for the first time from the West Atlantic. The taxonomic status of P. acanthonotus is discussed. A key to the Atlantic species of Plesionika is provided.
    Keywords: Caridea ; Pandalidae ; Plesionika ; range extensions ; West Atlantic ; Colombia ; key
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 10
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    Conformational flexibility of the oncogenic protein LMO2 primes the formation of the multi-protein transcription complex (2014)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2014-01-11
    Description: LMO2 was discovered via chromosomal translocations in T-cell leukaemia and shown normally to be essential for haematopoiesis. LMO2 is made up of two LIM only domains (thus it is a LIM-only protein) and forms a bridge in a multi-protein complex. We have studied the mechanism of formation of this complex using a single domain antibody fragment that inhibits LMO2 by sequestering it in a non-functional form. The crystal structure of LMO2 with this antibody fragment has been solved revealing a conformational difference in the positioning and angle between the two LIM domains compared with its normal binding. This contortion occurs by bending at a central helical region of LMO2. This is a unique mechanism for inhibiting an intracellular protein function and the structural contusion implies a model in which newly synthesized, intrinsically disordered LMO2 binds to a partner protein nucleating further interactions and suggests approaches for therapeutic targeting of LMO2. Scientific Reports 4 doi: 10.1038/srep03643
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Nature Publishing Group (NPG)
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