Note: Cover -- Half Title -- Title Page -- Copyright Page -- Dedication -- Table of Contents -- Preface -- Note to Reader -- Chapter 1: Nomenclature -- Reaction Components -- Kinetic Mechanism -- Kinetic Constants -- Microscopic Rate Constants -- Macroscopic Rate Constants -- Chapter 2: Introduction to Kinetics -- Chemical Kinetics -- First-Order Kinetics -- Second-Order Kinetics -- Pseudo-First-Order Kinetics -- Saturation Kinetics -- Temperature Dependence of Kinetic Parameters -- Steady-State Enzyme Kinetics -- Initial Rate -- Steady State -- The Michaelis-Menten/Briggs-Haldane Equation -- Overall Rate Equation -- Presentation of Initial Rate Data -- Chapter 3: Enzyme Assays -- Assays with Reduced Pyridine Nucleotides -- Fixed Time Assays -- Coupled Assays -- Analysis of Time Courses -- Chapter 4: Derivation of Initial Velocity Rate Equations and Data Processing -- Derivation of Rate Equations -- Algebraic Solution -- King-Altman Method -- Alternative Reaction Pathways -- Conversion of the Initial Velocity Rate Equation to Kinetic Constants -- Net Rate Constant Method -- Rapid Equilibrium Approximation -- Derivation of Equations for Isotope Exchange -- Derivation of Equations for Isotope Effects -- Shorthand Notation for Rate Constants -- Data Processing -- Chapter 5: Initial Velocity Studies in the Absence of Added Inhibitors -- Uni Bi Enzyme Reactions -- Bireactant Enzyme Reactions -- Ordered Sequential Mechanisms -- Equilibrium Ordered Mechanism -- Steady-State Ordered Mechanism -- Theorell-Chance Mechanism -- Random Sequential Reactions -- Dependence of Km on Reactant Concentration -- Ping Pong Reactions -- Determination of Vmax -- Can Sequential and Ping Pong Mechanisms Resemble One Another? -- Crossover Point Analysis -- Terreactant Enzyme Mechanisms -- No Constant Term-Ping Pong Mechanisms -- Constant Term Present-Sequential Mechanisms. , Methods for Telling Which Denominator Terms Are Missing -- Haldane Relationships -- Alternative Substrate Studies -- Kinetics of Metal Ions -- Cooperativity and Allosterism -- Positive Cooperativity -- Negative Cooperativity -- The Hill Plot -- Allosterism -- Kinetic Mechanism of Regulation -- Transmission of Allosteric Effects-Coupling of Active and Allosteric Sites -- Practical Considerations -- From Data to Interpretation -- Chapter 6: Initial Velocity Studies: Presence of Added Inhibitors -- Irreversible Step -- Types of Inhibition -- Competitive Inhibition -- Noncompetitive Inhibition -- Uncompetitive Inhibition -- Product Inhibition -- Uni Bi Reaction Mechanisms -- Uni Bi Steady-State Ordered -- Uni Bi Rapid Equilibrium Random -- Rules for Predicting Product Inhibition Patterns -- Bireactant Enzyme Mechanisms -- Ordered Kinetic Mechanisms -- Steady-State Ordered -- Theorell-Chance -- Equilibrium Ordered -- Random Kinetic Mechanisms -- Rapid Equilibrium Random with EBQ Dead-End Complex -- Steady-State Random -- Ping Pong Mechanisms -- Classical (One-Site) Ping Pong Mechanism -- Nonclassical (Two-Site) Ping Pong Mechanism -- Alternate Product Inhibition -- Dead-End Inhibition -- Rules for Predicting Dead-End Inhibition Patterns -- Combination of Inhibitor with More Than a Single Enzyme Form -- Mixed Product and Dead-End Inhibition -- Multiple Combinations of a Dead-End Inhibitor -- Substrate Inhibition -- Complete Substrate Inhibition -- Partial Substrate Inhibition -- Induced Substrate Inhibition -- Alternate Substrate Inhibition -- Ping Pong Mechanism -- Sequential Mechanisms -- Double Inhibition -- Slow Binding and Tight Binding Inhibition -- Slow Binding Inhibition -- Effect of a Reversible Inhibitor on a Slow Binding Inhibitor -- Tight Binding Inhibition -- Slow Tight Binding Inhibition -- Some Practical Considerations. , Chapter 7: Pre-Steady-State and Relaxation Kinetics -- Reactant Concentration in Excess of Enzyme Concentration -- Irreversible First-order Reactions -- Reversible First-order Reactions -- Consecutive First-order Reactions -- Parallel First-order Reactions -- Burst in a Time Course -- Reactant Concentration Comparable to Enzyme Concentration -- Temperature Jump -- Methods of Pre-Steady-State Analysis -- Rapid Spectral Acquisition -- Single Wavelength Methods -- Stopped Flow -- Rapid Quench -- Relaxation Methods -- Chapter 8: Isotopic Probes of Kinetic Mechanism -- Isotopic Exchange -- Isotopic Exchange at Equilibrium -- Ping Pong Exchange Patterns -- Exchange at Equilibrium in Sequential Mechanisms -- Isotope Exchange Not at Equilibrium -- Oversaturation and Iso Mechanisms -- Countertransport of Label -- Positional Isotopic Exchange (PIX) -- Determination of Stickiness -- Isotope Partitioning -- Experimental -- Data Analysis -- Theory -- Theoretical Limits -- Variation of V/K with Viscosity -- Chapter 9: Isotope Effects as a Probe of Mechanism -- Types of Isotope Effects -- Nomenclature -- Measurement of Kinetic Isotope Effects -- Direct Comparison of Initial Rates -- Equilibrium Perturbation -- Internal Competition -- Remote Label Method -- Types of Isotope Effects -- Equilibrium Isotope Effects -- Equations for Isotope Effects -- Calculation of Dissociation Constant -- Isotope Effects on More Than One Step -- Determination of Intrinsic Isotope Effects -- Northrop's Method -- Multiple Isotope Effect Method -- Multiple Isotope Effects in Stepwise Mechanisms -- Intermediate Partitioning -- Reactant Dependence of Isotope Effects -- Substrate Dependence of Isotope Effects in Bireactant Sequential Mechanisms -- Ordered Mechanisms -- Random Mechanisms -- Ping Pong Mechanisms. , Substrate Dependence of Isotope Effects in Terreactant and Higher Order Mechanisms -- Product Dependence of Isotope Effects -- Ordered Kinetic Mechanisms -- Random Kinetic Mechanisms -- Ping Pong Kinetic Mechanisms -- Isotope Effects as a Probe of Regulatory Mechanism -- Isotope Effects as a Probe of Chemical Mechanism -- Isotope Effects as a Probe of Transition-State Structure -- Formate Dehydrogenase -- Alcohol Dehydrogenase -- Glutamate Mutase -- Acyl and Phosphoryl Transfers -- Phosphoryl Transfer -- Glycosyl Transferases -- Binding Isotope Effects -- Transient-State Kinetic Isotope Effects -- Chapter 10: pH Dependence of Kinetic Parameters and Isotope Effects -- pH-Rate Studies -- Single Buffers with Overlap -- Mixed Buffer Systems -- pH Dependence of Equilibrium Dissociation Constant -- pH Dependence of V/K -- Activity Lost at Low pH -- Activity Lost at Low pH and pK1 above pH 7.3 -- Activity Lost at High pH -- Activity Lost at High pH and pK1 Less Than 7.3 -- V/K Profile Decreases at both Low and High pH -- V/K Profiles That Show Two pKs at Low or High pH -- Identifying the Groups Seen in V/K Profiles -- pH Dependence of Vmax -- V Profile with a Sticky Substrate and Proton -- pH Dependence of Vmax in a Ping Pong Mechanism -- Metal Ion Binding -- pKi Profiles for Metal Ions -- pH Dependence of Isotope Effects -- pH-Dependent Step is Sensitive to Isotopic Substitution -- Random Addition of Proton and Substrate to Enzyme -- Dead-End Protonation of Enzyme -- Dead-End Protonation of Enzyme and Enzyme-Reactant Complex -- Dead-End Formation of Protonated Enzyme-Reactant Complex -- DV in a Ping Pong Mechanism -- pH- and Isotope-Sensitive Steps Differ -- Appendices -- A1: King and Altman Patterns and Distribution Equations. , A2: Rate Equations, Definitions of Kinetic and Inhibition Constants, Haldanes, Distribution Equations, and Rate Constant Calculations for a Number of Multireactant Mechanisms -- Index.