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  • 1
    Electronic Resource
    Electronic Resource
    Quantization of longitudinal excitons in CuCl thin films (2001)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 90 (2001), S. 3662-3664 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The quantization of the exciton translational motion in CuCl thin films, having a thickness much less than the photon wavelength and overlying a substrate, is theoretically investigated by employing the 45° reflectometry. We show that the resonances associated with quantized longitudinal excitons can be directly measured by means of such a polarization-modulation technique despite the relatively large damping factors for very thin films. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1391419
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  • 2
    Electronic Resource
    Electronic Resource
    Infrared 45° reflectometry of very thin films (1999)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 86 (1999), S. 1404-1409 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: It is shown that the spectra given by the difference between the reflectivity for p-polarized light and the squared reflectivity for s-polarized light (Rp−Rs2) with an angle of incidence of 45° can be used for directly determining the frequencies of zone-center transverse optical (ωTO) and longitudinal optical (ωLO) phonons in very thin polar-semiconductor films on substrates. These spectra are also useful for measuring resonance frequencies of both transverse and longitudinal optical confined phonons in ultrathin layers of superlattices. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.370902
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  • 3
    Electronic Resource
    Electronic Resource
    Subcellular Distribution Studies of Diadenosine Polyphosphates—Ap4A and Ap5A—in Bovine Adrenal Medulla: Presence in Chromaffin Granules (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 51 (1988), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Diadenosine tetraphosphate (Ap4A) and diadenosine pentaphosphate (Ap5A) have been identified in bovine adrenal medullary tissue using an HPLC method. The values obtained were 0.1 ± 0.05 μmol/g of tissue for both compounds. The subcellular fraction where Ap4A and Ap5A were present in the highest concentration was chromaffin granules: 32 nmol/mg of protein for both compounds (∼6 mM intragranularly). This value was 30 times higher than in the cytosolic fraction. Enzymatic degradation of Ap4A and Ap5A, isolated from chromaffin granules, with phosphodiesterase produces AMP as the final product. The Ap4A and Ap5A obtained from this tissue were potent inhibitors of adenosine kinase. Their Ki values relative to adenosine were 0.3 and 2 μM for Ap4A and Ap5A, respectively. The cytosolic fraction also contains enzymatic activities that degrade Ap4A as well as Ap5A. These activities were measured by an HPLC method; the observed Km values were 10.5 ± 0.5 and 13 ± 1 μM for Ap4A and Ap5A, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1988.tb01147.x
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  • 4
    Electronic Resource
    Electronic Resource
    Histamine-Evoked Chromaffin Cell Scinderin Redistribution, F-Actin Disassembly, and Secretion: In the Absence of Cortical F-Actin Disassembly, an Increase in Intracellular Ca2+ Fails to Trigger Exocytosis (1995)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 65 (1995), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Histamine is a known chromaffin cell secretagogue that induces Ca2+-dependent release of catecholamines. However, conflicting evidence exists as to the source of Ca2+ utilized in histamine-evoked secretion. Here we report that histamine-H1 receptor activation induces redistribution of scinderin, a Ca2+-dependent F-actin severing protein, cortical F-actin disassembly, and catecholamine release. Histamine evoked similar patterns of distribution of scinderin and filamentous actin. The rapid responses to histamine occurred in the absence of extracellular Ca2+ and were triggered by release of Ca2+ from intracellular stores. The trigger for the release of Ca2+ was inositol 1,4,5-trisphosphate because U-73122, a phospholipase C inhibitor, but not its inactive isomer (U-73343), inhibited the increases in IP3 and intracellular Ca2+ levels, scinderin redistribution, cortical F-actin disassembly, and catecholamine release in response to histamine. Thapsigargin, an agent known to mobilize intracellular Ca2+, blocked the rise in intracellular Ca2+ concentration, scinderin redistribution, F-actin disassembly, and catecholamine secretion in response to histamine. Calphostin C and chelerythrine, two inhibitors of protein kinase C, blocked all responses to histamine with the exception of the release of Ca2+ from intracellular stores. This suggests that protein kinase C is involved in histamine-induced responses. The results also show that in the absence of F-actin disassembly, rises in intracellular Ca2+ concentration are not by themselves capable of triggering catecholamine release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1995.65031297.x
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  • 5
    Electronic Resource
    Electronic Resource
    Loss and Ca2+-Dependent Retention of Scinderin in Digitonin-Permeabilized Chromaffin Cells: Correlation with Ca2+-Evoked Catecholamine Release (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 59 (1992), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Exposure of chromaffin cells to digitonin causes the loss of many cytosolic proteins. Here we report that scinderin (a Ca2+-dependent actin-filament-severing protein), but not gelsolin, is among the proteins that leak out from digitonin-permeabilized cells. Chromaffin cells that were exposed to increasing concentrations (15–40 μM) of digitonin for 5 min released scinderin into the medium. One-minute treatment with 20 μ digitonin was enough to detect scinderin in the medium, and scinderin leakage levelled off after 10 min of permeabilization. Elevation of free Ca2+ concentration in the permeabilizing medium produced a dose-dependent retention of scinderin. Results were confirmed by immunofluorescence microscopy of digitonin-permeabilized cells. Subcellular fractionation of permeabilized cells showed that scinderin leakage was mainly from the cytoplasm (80%); the remaining scinderin (20%) was from the microsomal fraction. Other Ca2+-binding proteins released by digitonin and also retained by Ca2+ were calmodulin, protein kinase C, and calcineurins A and B. Scinderin leakage was parallel to the loss of the chromamn cell secretory response. Permeabilization in the presence of increasing free Ca2+ concentrations produced a concomitant enhancement in the subsequent Ca2+-dependent catecholamine release. The experiments suggest that: (1) scinderin is an intracellular target for Ca2+, (2) permeabilization of chromaffin cells with digitonin in the presence of micro-molar Ca2+ concentrations retained Ca2+-binding proteins including scinderin, and (3) the retention of these proteins may be related to the increase in the subsequent Ca2+-dependent catecholamine release observed in permeabilized chromaffin cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1992.tb11003.x
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of Electron Beam Irradiation on the Bacterial Load and Sensorial Quality of Sliced Cantaloupe (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Electron beam irradiated sliced cantaloupe was tested for 21 d of storage for total aerobic microbial counts, texture, color, and different sensorial parameters as a function of irradiation doses 0, 0.7, and 1.4 kGy and the wash treatments, 1 and 200 ppm chlorine applied to the melons before cutting. Irradiation resulted in a reduction in the total aerobic microbial counts with increasing doses. Melons washed only with water before cutting had total aerobic bacterial counts of 4.0, 2.0, and 0.8 log colony-forming units (CFU)/g on day 0 at irradiation doses of 0, 0.7, and 1.4 kGy, respectively. Across all doses of irradiation, counts were consistently lower for cantaloupe pieces obtained from melons that had been subjected to chlorine rinse in comparison with those washed with water without chlorine. Melons washed with chlorine before cutting had total aerobic bacterial counts of 2.7, 0.7, and 0.5 log CFU/g on day 0 at irradiation doses of 0, 0.7, and 1.4 kGy, respectively. Objective color analysis indicated no significant effect of irradiation on the color of cantaloupe. Texture measured as compression force was lower only for cantaloupe irradiated at 1.4 kGy. Irradiation did not affect descriptive attribute flavor and texture sensory attributes of cantaloupe pieces. Decontamination of whole cantaloupes before cutting using chlorine wash may be combined with low-dose irradiation for shelf-life extension of sliced cantaloupe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb09941.x
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  • 7
    Electronic Resource
    Electronic Resource
    Accurate spectroscopic constants for the Cd(1S,3P,1P)–H2 van der Waals complexes: A theoretical study (1994)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 100 (1994), S. 8251-8256 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Very accurate interaction energies and equilibrium geometries are obtained for the C2v Cd(1S, 3P, 1P)–H2 van der Waals (VDW) complexes using extensive ab initio multireference configuration interaction plus second order multireference Möller–Plesset calculations. Gaussian basis sets are of triple-ζ plus polarization quality and a relativistic effective core potential for cadmium has been used. For the interaction energies the Boys and Bernardi counterpoise (CP) correction has been used. The ground state Cd(1S)–H2 van der Waals complex was found to be very weakly bound with a 108 cm−1 well at 8.4 a.u., as compared with the estimated experimental values of De (approximately-equal-to)50 cm−1 at 8.3 a.u. The computed (De=1580 cm−1, Re=4.9 a.u.) constants for the 1B1 Cd(1 P)–H2 exciplex are in good agreement with the recent experimentally derived values (De=1713 cm−1, Re=4.7 a.u.) obtained by Wallace et al. [J. Chem. Phys. 97, 3135 (1992)]. The well depth and equilibrium geometry for the 3B1 Cd (3P) –H2 exciplex were computed as 420 cm−1 and 5.7 a.u. while those of the 3B2 exciplex are 650 cm−1 and 4.75 a.u. The 1B2 surface did not lead to a VDW complex but instead it produced a strongly bound (De(approximately-equal-to)10 kcal/mol) bent H–Cd–H molecule responsible for the experimentally observed CdH+H and Cd+H+H reaction channels. The 3,1A1 states arising from the Cd(3,1P)+H2 asymptotes were found to be totally repulsive. In all cases the CP correction was a non-negligible fraction of the interaction energy. Finally, the energetic position and the geometry of the 1B1/3A1 surface crossing, crucial to explain the observed Cd(1B1,v')–H2 predissociation lifetimes, are accurately determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.466768
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  • 8
    Electronic Resource
    Electronic Resource
    Segregation distortion for agronomic traits in doubled haploid lines of barley (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four barley doubled haploid populations were produced by anther culture from the reciprocal crosses between two six-row barley cultivars, ‘Plaisant’ and ‘Orria’; the doubled haploid lines (DHLs) derived from each cross were subsequently assigned to weak or vigorous populations according to the weak or vigorous nature of the originating embryos. Well-formed embryos at day 25 on the induction medium were considered vigorous, whereas embryos maturing later were considered weak. The classification of vigorous and weak was closely associated with the ratio of green to albino plantlets regenerated. A random set of 25 DHLs from each of the four populations were selected for field testing in a replicated trial. Furthermore, a second set consisting of a total of 454 unreplicated DHLs from the four populations were also field assessed for grain yield. Distortion during in vitro culture may impede regeneration of a random array of microspores from a given cross, and may bias genetic estimates of specific trait/marker association in genetic studies. However, no significant differences were detected in this study among the four populations for days to heading, height, grain yield and thousand-kernel-weight when measured on the replicated trial of 100 DHLs, nor for grain yield in the second collection of 454 entries. This suggests that the likelihood of producing improved agronomic pure lines is independent of the direction of crossing and, more importantly, independent of the time when embryos matured in the induction media, at least for these particular six-row cultivars and for the anther culture method used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2005.01159.x
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular engineering of a hexagonal columnar (.PHI.h) mesophase exhibited by flexible copolyethers based on 1-(4-hydroxyphenyl)-2-(2-R-4-hydroxyphenyl)ethane with R = H, F, and flexible spacers (1992)
    s.l. : American Chemical Society
    Macromolecules 25 (1992), S. 1193-1197 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00029a029
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  • 10
    Electronic Resource
    Electronic Resource
    C3v versus C2v Cd(1S,3P,1P)–CH4 van der Waals complexes: A variational and perturbational multireference configuration interaction study (1993)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 98 (1993), S. 8065-8069 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The Cd(1S,3,1P)–CH4 interaction is studied for the C3v vertex-on Cd(1S,3,1P)–HCH3 and face-on Cd(1S,3,1P)–H3CH approaches as well as the C2v Cd(1S,3,1P)–H2CH2 edge-on approach of the metal atom to methane using extensive ab initio multireference configuration interaction plus second order multireference Møller–Plesset calculations. The ground state Cd(1S)–CH4 is totally repulsive for the C3v vertex-on and C2v edge-on approaches. Only a stable face-on Cd(1S)–H3CH complex was found. Although vertex-on and edge-on minima were found for the Cd(3P)–CH4 excited complexes, they are less attractive than the corresponding Cd(3P)–H3CH face-on exciplexes. The optimal orientation of the face-on occupied 5p orbital of Cd(5s5p) is that which minimizes the overlap with C–H bonds leading to a "3Πy'' exciplex. For the Cd(1P)–CH4 interaction, two face-on and two edge-on very stable complexes (all with De≥1300 cm−1) were found. The Cd(1S)–H3CH (1A1) complex was found to have a 185 cm−1 well at 8.03 a.u. Both excited P states present shorter equilibrium distances—the Cd(3P)–H3CH (3E) exciplex has a 799 cm−1 well at 6.08 a.u., while the Cd(1P)–H3CH exciplex (1E) has a 1337 cm−1 well at 5.57 a.u. These results are in good agreement with the recent experimental values X 1O+(1S)—(De=121 cm−1, Re=7.9 a.u.); A 3O+(3P)—(De=677 cm−1, Re=5.80 a.u.); and 1P—(De(approximately-equal-to)1300 cm−1, Re=5.59 a.u.) obtained by Wallace and Breckenridge [J. Chem. Phys. 97, 2318 (1992)]. The present work confirms the C3v facial nature of the observed (1S) ground and (3P) excited complexes. The present results are inconclusive as to the facial or edge-on nature of the Cd(1P)–CH4 exciplex, but they preclude the C3v vertex-on possibility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.464562
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