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  • 1
    ISSN: 1432-0983
    Keywords: Yeast ; 2 μm plasmid ; Mitotic recombination ; Coincident conversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of the 2 μm plasmid on homologous recombination in the right arm of chromosome XV of the yeast Saccharomyces cerevisiae has been examined. No differences between spontaneous mitotic recombination rates in [cir 0] and [cir +] derivatives of two yeast diploid tester strains were detected. In the course of analysis an unusually high coincident conversion frequency at ADE2, HIS3, and two RFLP loci adjacent to ADE2, was observed. The character of coincident homozygotization of linked markers argues for a “break-and-replicate” mechanism underlying the coincident conversion events.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Episomal plasmid ; Chromosomal integration ; Plasmid rescue ; Gene seduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Yeast diploids containing a chromosomally integrated episomal plasmid manifest the mitotic instability of the chromosomes with the plasmid. Probably as a results of the destabilization integrated plasmid may be excised out of the chromosome. It was found that the rescue of the plasmid may be irregular, with the taking up of adjacent chromosomal gene. Using an integrant with the plasmid integrated into chromosome I very near the ADE1 locus we cloned this gene “in vivo” by selecting rescued plasmid marker LEU2. The new plasmid has retained the LEU2 gene and the capacity to replicate autonomously. This plasmid was used to transfer the cloned ADEI gene from one cell to another by transformation and from one resident chromosome to another by integration. The phenomenon of irregular excision of an integrated episomal plasmid together with linked chromosomal gene(s) and transfer to other chromosomes or cells we propose to term Seduction.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2019-05-20
    Description: The question "Are we alone in the Universe?" is perhaps the most fundamental one that affects mankind. How can we address the search for life in our Solar System? Mars, Enceladus and Europa are the focus of the search for life outside the terrestrial biosphere. While it is more likely to find remnants of life (fossils of extinct life) on Mars because of its past short time window of the surface habitability, it is probably more likely to find traces of extant life on the icy moons and ocean worlds of Jupiter and Saturn. Nevertheless, even on Mars there could still be a chance to find extant life in niches near to the surface or in just discovered subglacial lakes beneath the South Pole ice cap. Here, the different approaches for the detection of traces of life in the form of biosignatures including pre-biotic molecules will be presented. We will outline the required infrastructure for this enterprise and give examples of future mission concepts to investigate the presence of life on other planets and moons. Finally, we will provide suggestions on methods, techniques, operations and strategies for preparation and realization of future life detection missions.
    Type: Conference or Workshop Item , NonPeerReviewed
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