GLORIA

GEOMAR Library Ocean Research Information Access

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Norfolk :Caister Academic Press,
    Keywords: Polymerase chain reaction. ; Electronic books.
    Description / Table of Contents: This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. Topics covered include: principles of PCR, fluorescent chemistries, instrumentation, quantification strategies, extraction and purification of nucleic acids, sample preparation, controls for validation, primers and probes, standardization of methods, MIQE guidelines, mRNA expression and PCR arrays. This book provides a comprehensive overview of PCR theory, instrumentation and methods. The book represents an excellent, detailed guide for anyone interested in the development and use of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.
    Type of Medium: Online Resource
    Pages: 1 online resource (269 pages)
    Edition: 1st ed.
    ISBN: 9781912530250
    DDC: 547.28
    Language: English
    Note: Intro -- Contents -- Chapter 1 Introduction to the Real-time Polymerase Chain Reaction -- Chapter 2 Principles of the Real-time Polymerase Chain Reaction -- Chapter 3 Homogenous Fluorescent Chemistries for Real-time PCR -- Chapter 4 Instrumentation and Fluorescent Chemistries Used in Quantitative Polymerase -- Chapter 5 Quantification Strategies in Real-time Polymerase Chain Reaction -- Chapter 6 The Extraction and Purification of Nucleic Acids for Analysis by PCR -- Chapter 7 Sample Preparation for Real-time PCR in Food Science -- Chapter 8 Internal and Other Controls for Real-time PCR Validation -- Chapter 9 Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase -- Chapter 10 Internal Amplification Controls in Real-time Polymerase Chain Reaction-Based -- Chapter 11 Standardization of Real-time PCR Methods in Food Microbiology -- Chapter 12 MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay -- Chapter 13 Analysis of mRNA Expression by Real-time PCR -- Chapter 14 Real-time PCR Arrays.
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 2
    Online Resource
    Online Resource
    La Vergne :Royal Society of Chemistry, The,
    Keywords: DNA-drug interactions. ; DNA-binding proteins. ; Gene targeting. ; Drug targeting. ; Chemotherapy. ; Electronic books.
    Description / Table of Contents: This book discusses diverse modes of binding of antibiotics and drugs to DNA, emphasising matters that are important or promising for cancer treatment.
    Type of Medium: Online Resource
    Pages: 1 online resource (271 pages)
    Edition: 1st ed.
    ISBN: 9781847555304
    Series Statement: Issn Series
    DDC: 572.8633
    Language: English
    Note: Sequence-Specific DNA Binding Agents -- Contents -- Chapter 1 DNA Recognition by Triple Helix Formation -- 1.1 Introduction -- 1.1.1 Triplets and Triplex Motifs -- 1.2 Strategies to Increase Triplex Stability -- 1.2.1 Sugar Modifications -- 1.2.2 Addition of Positive Charges -- 1.2.3 Backbone Modifications -- 1.2.4 Base Stacking -- 1.2.5 Triplex-Binding Ligands -- 1.3 Overcoming the pH Dependency -- 1.3.1 Pyrimidine Base Analogues -- 1.3.2 Purine Base Analogues -- 1.4 Recognition of Pyrimidine Interruptions -- 1.4.1 Null Bases and Abasic Linkers -- 1.4.2 Natural Bases -- 1.4.3 Nucleotide Analogues for Recognizing Pyrimidine Interruptions -- 1.4.4 Nucleotide Analogues for Recognizing both Partners of the Base Pair -- 1.5 Mixed Sequence Recognition -- Acknowledgements -- References -- Chapter 2 Interfacial Inhibitors of Human Topoisomerase I -- 2.1 Introduction -- 2.2 Molecular Mechanism of Action of Drugs that Trap Top1 Cleavage Complexes -- 2.2.1 Intercalation between the Base Pairs Flanking the Top1-Mediated DNA Break -- 2.2.2 DNA Untwisting by Drugs at the Top1-Mediated DNA Cleavage Site -- 2.2.3 Common Hydrogen-Bond Network for Top1 Inhibitors Bound in the Ternary Complex -- 2.3 Generalization of the Interfacial Inhibitor Concept -- Acknowledgments -- References -- Chapter 3 Diversity of Topoisomerase I Inhibitors for Cancer Chemotherapy -- 3.1 Introduction -- 3.2 Camptothecins -- 3.3 Indenoisoquinolines -- 3.4 Benzimidazoles -- 3.5 Indolocarbazoles -- 3.6 Phenanthridines and Related Compounds -- 3.7 Marine Alkaloids -- 3.8 Plant Natural Products -- 3.9 Conclusion -- References -- Chapter 4 Slow DNA Binding -- 4.1 Introduction - Kinetics vs. Thermodynamics of DNA Binding -- 4.2 Different DNA-Binding Modes - Different DNA-Binding Kinetics -- 4.2.1 External Electrostatic Binding -- 4.2.2 Groove Binding -- 4.2.3 Intercalation. , 4.2.4 Threading Intercalation -- 4.3 Common Slow DNA Binders -- 4.3.1 Actinomycin D -- 4.3.2 Nogalamycin -- 4.4 Ruthenium Complexes Exhibiting Slow DNA Binding Kinetics -- 4.4.1 Bis-intercalating Ru-dimer [μ-c4(cpdppz)2(phen)4Ru2]4+ -- 4.4.2 Semirigid Ru-dimer [μ-(11,110-bidppz)(x)4Ru2]4+ (x=phen or bipy) -- References -- Chapter 5 DNA Gene Targeting using Peptide Nucleic Acid (PNA) -- 5.1 Introduction -- 5.2 Duplex DNA Recognition in vitro -- 5.3 PNA Conjugates -- 5.4 Effect of PNA Binding on DNA Structure -- 5.5 Cellular Gene Targeting -- 5.6 Activation of Gene Transcription -- 5.7 Gene-Targeted Repair -- 5.8 Cellular Delivery and Bioavailability in vivo -- 5.9 Prospects -- References -- Chapter 6 Actinomycin D: Sixty Years of Progress in Characterizing a Sequence-Selective DNA-Binding Agent -- 6.1 Summary -- 6.2 Introduction -- 6.2.1 Historical Perspectives -- 6.3 DNA-Binding Studies: The Early Years -- 6.3.1 The Intercalation Model -- 6.3.2 Sequence-Selectivity of Actinomycin D -- 6.4 Characterization of the Actinomycin D-DNA Complex -- 6.4.1 Role of Bases Flanking the Actinomycin D-Binding Site -- 6.4.2 Promiscuity in the Sequence Selectivity of Actinomycin D -- 6.5 Global vs. Microscopic Sequence-Recognition -- 6.5.1 The Shuffling Hypothesis Revisited -- 6.6 Structural Motifs as Actinomycin D Targets -- 6.6.1 The Era of Single-Strand DNA Binding -- 6.7 Conclusions -- Acknowledgments -- References -- Chapter 7 Thermal Denaturation of Drug-DNA Complexes: Tools and Tricks -- 7.1 Introduction -- 7.2 Thermal Denaturation Tools -- 7.2.1 Analysis of Tm Shifts in the Presence of Drug -- 7.2.2 Obtaining Binding Enthalpy Values by DSC -- 7.2.3 Modeling Melting Curves by McGhee's Algorithm -- 7.2.4 Case Studies: Bisintercalating Anthracyclines and Echinomycin -- 7.2.5 Summary: Advantages and Pitfalls -- 7.3 Thermal Denaturation: New Tricks. , 7.3.1 Melting Mixtures to Assess Sequence- and Structural-Selectivity -- 7.3.2 Advantages and Prospects -- 7.4 Summary -- Acknowledgments -- References -- Chapter 8 Computer Simulations of Drug-DNA interactions: A Personal Journey -- 8.1 Introduction -- 8.2 Minor Groove DNA Binders -- 8.3 Natural Bifunctional Intercalators and Hoogsteen Base Pairing -- 8.4 Bis-Intercalation of Echinomycin and Related Bifunctional Agents in Relation to Binding Sequence Preferences -- 8.5 Binding Preferences of Synthetic Pyridocarbazole Bis-Intercalators -- 8.6 Sequence Selectivity of Actinomycin D -- 8.7 Binding of the Potent Antitumour Agent Trabectedin to DNA -- 8.8 Lamellarins as Topoisomerase I Poisons -- 8.9 Concluding Remarks -- Acknowledgements -- References -- Chapter 9 The Discovery of G-Quadruplex Telomere Targeting Drugs -- 9.1 Introduction -- 9.2 Anthraquinones and Intercalation into Duplex DNA -- 9.3 Interactions with Higher-Order DNA -- 9.4 Telomerase and Cancer -- 9.5 First-Generation G-Quadruplex Ligands -- 9.6 Molecular Models for Quadruplex-Trisubstituted Acridine Complexes -- 9.7 Cellular and Pharmacological Properties of Trisubstituted Acridines -- 9.8 Conclusions -- Acknowledgements -- References -- Chapter 10 The Mechanism of Action of Telomestatin, a G-Quadruplex-Interactive Compound -- 10.1 Introduction -- 10.1.1 Telomere Structure in Mammals and Telomerase -- 10.1.2 Mechanism of Inhibition of Telomerase by Telomestatin -- 10.1.3 The Stoichiometry of Binding of Telomestatin to the Human Telomeric G-Quadruplex -- 10.1.4 Identity of the Telomeric G-Quadruplex Formed in the Presence of Telomestatin -- 10.1.5 Proposed Models for Telomestatin Binding to the Human Telomeric G-Quadruplex Structure -- 10.1.6 Potential Effect of Telomestatin on the Assembly of Telomeres into Higher-Order Structures. , 10.1.7 Genomic Instability Caused by Telomestatin Treatment and Activation of DNA Damage Response -- 10.1.8 Other Mechanisms of Telomestatin in Mediating its Biological Activity -- 10.2 Concluding Remarks -- Acknowledgments -- References -- Chapter 11 Structural Features of the Specific Interactions between Nucleic Acids and Small Organic Molecules -- 11.1 Introduction -- 11.2 Diels-Alder Ribozymes -- 11.3 Theophylline and Flavin Mononucleotide Binding -- 11.4 Purine Riboswitches -- 11.5 Adenosine Monophosphate Binding -- 11.6 Conclusions -- 11.7 Perspectives -- References -- Subject Index.
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 3
    Online Resource
    Online Resource
    La Vergne :Royal Society of Chemistry, The,
    Keywords: DNA -- Synthesis. ; DNA replication. ; Electronic books.
    Description / Table of Contents: Written by leaders in the field, this volume describes the preparation and application of DNA-conjugates. Several have been used as sensors whilst others link reporter groups such as proteins or fluorophores to RNA or DNA for detection, single molecule studies, and increasing the sensitivity of PCR.
    Type of Medium: Online Resource
    Pages: 1 online resource (317 pages)
    Edition: 1st ed.
    ISBN: 9781849734936
    Series Statement: Issn Series
    DDC: 572.86
    Language: English
    Note: DNA Conjugates and Sensors -- Contents -- Chapter 1 Fluorophore-functionalised Locked Nucleic Acids (LNAs) -- 1.1 Introduction -- 1.2 LNA - a Primer -- 1.3 Fluorophore-functionalised LNA - an Overview -- 1.3.1 Introduction -- 1.3.2 N2'-Functionalised 2'-amino-LNA -- 1.3.3 N2'-Functionalised 2'-amino-α-L-LNA -- 1.3.4 C5-Functionalised LNA -- 1.3.5 LNA with Fluorescent Nucleobase Surrogates -- 1.4 Applications of Fluorophore-functionalised LNA -- 1.4.1 Formation of Pyrene Arrays -- 1.4.2 Hybridisation Probes -- 1.4.3 Base Discriminating Fluorescent Probes -- 1.4.4 Excimer-based Probes -- 1.5 Summary and Perspective -- References -- Chapter 2 Fluorophore Conjugates for Single Molecule Work -- 2.1 Introduction -- 2.1.1 Single Molecule Detection Techniques -- 2.2 Labelling DNA for Single Molecule Detection -- 2.2.1 Selection of Fluorophores -- 2.2.2 Dye Photophysics -- 2.2.3 Labelling Strategies -- 2.3 Applications -- 2.3.1 DNA Sequence Analysis -- 2.3.2 Elucidating DNA Structure and Function -- 2.4 Conclusions -- Acknowledgements -- Con ict of Interest Statement -- References -- Chapter 3 Small Molecule-Oligonucleotide Conjugates -- 3.1 Introduction -- 3.2 The Recognition of Nucleic Acids by Synthetic Oligonucleotides -- 3.2.1 Duplex Formation and the Antisense Strategy -- 3.2.2 Triplex Formation and the Antigene Strategy -- 3.3 Small Molecule-Oligonucleotide Conjugation -- 3.4 Improving the Hybridisation Properties of Oligonucleotides -- 3.4.1 Stabilisation by Edge Binders -- 3.4.2 Stabilisation by Intercalators -- 3.4.3 Stabilisation by Groove Binders -- 3.5 Cross-linking Oligonucleotides to Nucleic Acid Targets -- 3.5.1 Photoactive Cross-linkers -- 3.5.2 Alkylating and Alkylating-like Cross-linkers -- 3.6 Oligonucleotide-directed Cleavage of Nucleic Acid Targets -- 3.6.1 Metal Complexes -- 3.6.2 Topoisomerase Inhibitors. , 3.7 Summary and Future Directions -- References -- Chapter 4 Small Molecule-RNA Conjugates -- 4.1 Introduction -- 4.2 Small Molecule-RNA Conjugates -- 4.2.1 Examples of Naturally Occurring Small Molecule-RNA Conjugates -- 4.2.2 Synthetic Small Molecule-RNA Conjugates -- 4.2.3 Other Synthetic Small Molecule-RNA Conjugates -- 4.3 Concluding Remarks -- References -- Chapter 5 Click Chemistry - a Versatile Method for Nucleic Acid Labelling, Cyclisation and Ligation -- 5.1 Introduction to Click Chemistry -- 5.2 The CuAAC Reaction for Oligonucleotide Labelling -- 5.3 Reverse Click Labelling of Oligonucleotides by the CuAAC Reaction -- 5.4 DNA Labelling in vivo -- 5.5 Other Click Reactions for Oligonucleotide Labelling -- 5.6 Click Chemistry for Oligonucleotide Strand Ligation -- 5.7 Click Chemistry in Nanotechnology -- 5.8 Synthesis of Cyclic DNA -- 5.9 The SPAAC Reaction on DNA -- 5.10 Conclusion -- Acknowledgements -- References -- Chapter 6 Therapeutic Applications of Nucleic Acid Aptamer Conjugates -- 6.1 Introduction -- 6.2 Alternative Chemistries Improve Oligonucleotide Stability, Bioavailability and Distribution -- 6.2.1 Nucleotide Modi cation -- 6.2.2 End Modi cation -- 6.3 Oligonucleotide Conjugates as Novel Targeted Therapies -- 6.3.1 Aptamer Smart Drugs -- 6.3.2 Oligonucleotide Hybrids and Chimeras -- 6.3.3 Aptamers Delivering Toxic Cargo -- 6.3.4 Aptamer-targeted Delivery Vehicles -- 6.4 Oligonucleotide Conjugates as Imaging Reagents -- 6.5 Nanoparticles -- 6.6 Toxicology -- 6.7 Perspectives -- References -- Chapter 7 pH Sensitive DNA Devices -- 7.1 Introduction -- 7.2 Triplexes -- 7.2.1 Structural Features of Triplexes -- 7.2.2 Molecular Devices Based on Triplexes -- 7.3 i-Motifs -- 7.3.1 Structural Features of i-Motifs -- 7.3.2 Molecular Devices Based on i-Motifs -- 7.4 P-Helix and A-Motifs. , 7.4.1 Structural Characteristics of Poly rA and Poly dA Oligomers -- 7.4.2 Molecular Devices Based on A-Motifs -- 7.5 Outlook -- References -- Chapter 8 Making Sense of Catalysis: The Potential of DNAzymes as Biosensors -- 8.1 Introduction -- 8.1.1 Discovery of DNAzymes through in vitro Selection -- 8.1.2 RNA-Cleaving DNAzymes -- 8.1.3 Other Reactions Catalysed by DNAzymes -- 8.2 Fluorescent DNAzyme-based Sensors -- 8.2.1 DNAzyme-Fluorophore Conjugates -- 8.2.2 Fluorescent Sensors using Existing DNAzymes -- 8.2.3 Isolation of de novo Fluorescent DNAzyme Sensors through in vitro Selection -- 8.2.4 Fluorescence-generating Aptazymes: Linking Target Recognition to Catalytic Activity -- 8.2.5 Using DNAzymes to Amplify Fluorescent Signals -- 8.3 DNAzyme Sensors with Other Reporting Systems -- 8.3.1 Gold Nanoparticle-based Sensors -- 8.3.2 Electrochemical-based Sensors -- 8.4 Conclusions and Future Prospects -- References -- Chapter 9 Electrochemical Techniques as Powerful Readout Methods for Aptamer-based Biosensors -- 9.1 Introduction to Aptamer-based Sensors (Aptasensors) -- 9.1.1 Aptamers -- 9.1.2 Aptamers as Biosensors -- 9.2 Electrochemical Aptasensors -- 9.2.1 Introduction to Electrochemical Transduction -- 9.2.2 Electrochemical Aptasensors -- 9.3 Conclusions and Future Prospects -- Acknowledgements -- References -- Chapter 10 Oligonucleotide Conjugates for Detection of Speci c Nucleic Acid Sequences -- 10.1 Introduction -- 10.2 Linear Probes -- 10.3 Binary (Split) Probes -- 10.4 Molecular Beacons -- 10.5 Conclusion -- References -- Chapter 11 Nucleic Acid-Nanoparticle Conjugate Sensors for Use with Surface Enhanced Resonance Raman Scattering (SERRS) -- 11.1 Introduction -- 11.1.1 SERS -- 11.1.2 SERRS -- 11.1.3 Suitable SE(R)RS Surfaces -- 11.1.4 Nanoparticles -- 11.2 DNA Detection Methods Based on SE(R)RS -- 11.2.1 Colloid-based Detection Techniques. , 11.2.2 Bulk Substrate-based Detection Techniques -- 11.3 Conclusion -- 11.4 Forward Outlook -- References -- Chapter 12 Covalent and Non-covalent Conjugates of Oligonucleotides as Arti cial Restriction DNA Cutters -- 12.1 Introduction -- 12.2 Ce(IV)/EDTA as Molecular Scissors Showing Remarkable Substrate-Speci city -- 12.2.1 Combination of Oligonucleotide Additives and Ce(IV)/EDTA for Site-Selective Scission of Single-Stranded DNA -- 12.2.2 Promotion of Site-Selective Scission by Conjugating Oligonucleotide with a Multiphosphonate Ligand -- 12.2.3 Scission of Human Telomeric Overhang DNA -- 12.2.4 Use of Peptide Nucleic Acid (PNA) to Prepare Arti cial Enzymes for Site-Selective Scission of Double-Stranded DNA -- 12.3 Preparation of an Arti cial DNA Cutter from Ce(III) as a Precursor of Catalytically Active Ce(IV) -- 12.4 Conclusion -- Acknowledgements -- References -- Subject Index.
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 4
    Online Resource
    Online Resource
    München : acatech - Deutsche Akademie der Technikwissenschaften | Halle (Saale) : Deutsche Akademie der Naturforscher Leopoldina | Mainz : Union der deutschen Akademien der Wissenschaften
    Keywords: Klimaneutralität ; Energieversorgung ; Industrieproduktion ; Szenariotechnik ; Deutschland ; Klimaschutz ; Umweltbezogenes Management ; Umweltfaktor ; Umweltschutzmarkt ; Konflikt ; Ökologie ; Umweltökonomie ; Technologie ; Kooperation ; Umwelttechnik ; Umweltbilanz ; Ökologisches Gleichgewicht ; Biotechnologie ; Planungsprozess ; Ökologischer Fußabdruck ; Kohlenstoff ; Carbon dioxide capture and storage ; Deutschland ; Graue Literatur ; Forschungsbericht ; Erneuerbare Energien ; Energiewende ; Strategie ; Grüner Wasserstoff ; Windenergie ; Sonnenenergie
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (224 Seiten, 12,81 MB) , Diagramme, Illustrationen
    ISBN: 9783982005355
    Series Statement: Schriftenreihe Energiesysteme der Zukunft
    Parallel Title: In Beziehung stehende Manifestation Wie wird Deutschland klimaneutral?
    Language: German
    Note: Förderkennzeichen BMBF 03EDZ2016 , Projektlaufzeit: 03/2016 bis 12/2023 , Literaturverzeichnis: Seite 208-216 , Volltext: PDF , Glossar: Seite 8-11
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 5
    Keywords: Forschungsbericht
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (48 Seiten, 3,92 MB) , Diagramme, Karte
    Language: German
    Note: Förderkennzeichen BMBF 01QE1145. - Verbund-Nummer 01114005 , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 6
    Keywords: Forschungsbericht
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (12 Seiten, 129,24 KB)
    Language: German
    Note: Förderkennzeichen BMBF 03EK3055E , Verbundnummer 01184654 , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 30 (1991), S. 1372-1385 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 31 (1992), S. 6390-6395 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...