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  • 1
    Electronic Resource
    Electronic Resource
    A semi-quantitative direct contact assay using fluorescein diacetate and L929 mouse fibroblasts in monolayer culture (1993)
    Springer
    Methods in cell science 15 (1993), S. 165-170 
    Details
    ISSN: 1573-0603
    Keywords: in vitro ; fluorescent dyes ; cell culture ; cytotoxicity ; L929
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A semi-quantitative procedure is described for measuring cell viability after short-term exposure to a test substance using a monolayer culture. Test substances are placed in direct contact with cell monolayers for various time intervals. The substances are removed and the monolayers are incubated in the presence of fluorescein diacetate. Monolayers are viewed under a fluorescent microscope and the percentage of fluorescing (viable) cells is estimated. The method is suitable for examining cytotoxic effects at short times of exposure and for discriminating between test substances that give similar, low toxicity endpoints in standard 24-h assays.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388271
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Susceptibility of normal and X-irradiated animals toAeromonas hydrophila infections (1986)
    Springer
    Current microbiology 13 (1986), S. 129-132 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The virulence of threeAeromonas hydrophila strains isolated from humans and water were compared by intramuscular inoculation of normal and x-irradiated mice and goldfish. Selected strains, irrespective of source, were pathogenic for mice and goldfish with maximum mortality of normal hosts observed at 24–48 h after injection. Immunosuppression resulted in increased mortality of hosts by two of the threeA. hydrophila strains. The variation in virulence potential of differentA. hydrophila isolates may depend upon the in vivo interaction of endotoxins, surface antigens, and extracellular bacterial products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01568507
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    Paper (German National Licenses)
    Fulltext
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