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  • 1
    Book
    Book
    Amsterdam [u.a.] : Elsevier
    Keywords: Nitrogen cycle ; Denitrification ; hardback ; academic/specialist ; Aufsatzsammlung ; Stickstoffkreislauf
    Type of Medium: Book
    Pages: XVII, 427 S. , Ill., graph. Darst.
    Edition: 1. ed.
    ISBN: 0444528571 , 9780444528575
    RVK:
    Language: English
    Note: Literaturangaben , Introduction to the biochemistry and molecular biology of denitrification / Rob J. M. van Spanning, David J. Richardson, Stuart J. FergusonThe prokaryotic nitrate reductases / David J. Richardson, Rob J. M. van Spanning, Stuart J. Ferguson -- Nitrite reductases in denitrification / Serena Rinaldo, Francesca Cutruzzolà -- Nitric oxide reductase : structural variations and catalytic mechanism / Simon de Vries, Suharti A. M. Pouvreau, Laurice A. M. Pouvreau -- Nitrous oxide reductases / Walter G. Zumft, Heinz Korner -- Denitrification in rhizobia-legume symbiosis / Maria J. Delgado, Sergio Casella, Eulogio J. Bedmar -- The dissimilatory reduction of nitrate to ammonia by anaerobic bacteria / Sudesh B. Mohan, Jeff A. Cole -- Physiology, biochemistry, and molecular biology of nitrogen fixation / William E. Newton -- Regulatory cascades to express nitrogenases / Bernd Masepohl, Karl Forchhammer -- The rhizobium-legume nitrogen-fixing symbiosis / Gary Stacey -- Plant symbioses with frankia and cyanobacteria / Katharina Pawlowski, Birgitta Bergman -- Associative nitrogen fixation / Anne Van Dommelen, Jos Vanderleyden -- Measuring N₂ fixation in the field / Jonathan P. Zehr, Joseph P. Montoya -- Biochemistry and molecular biology of nitrification / Stuart J. Ferguson, David J. Richardson, Rob J. M. van Spanning -- The ecology of nitrifying bacteria / Jim I. Prosser -- Anammox / Huub J. M. Op den Camp, Mike S. M. Jetten, Marc Strous -- Nitrate assimilation in bacteria / Conrado Moreno-Vivian, Enrique Flores -- Nitrate assimilation in plants / Rudolf Tischner, Werner Kaiser -- Characterization of proteolytic microbes and their activities in soils / Mirna Mrkonjic Fuka, Marion Engel, Jean-Charles Munch, Michael Schloter -- Molecular tools to assess the diversity and density of denitrifying bacteria in their habitats / Sara Hallin, Gesche Braker, Laurent Philippot -- Denitrification and agriculture / Jean Charles Munch, Gerard L. Velthof -- Denitrification and N-cycling in forest ecosystems / Per Ambus, Sophie Zechmeister-Boltenstern -- Denitrification in wetlands -- Oswald Van Cleemput, Pascal Boeckx, Per-Eric Lindgren, Karin Tonderski -- Organisms of the nitrogen cycle under extreme conditions : low temperature, salinity, pH value and water stress / Blaz̆ Stres ...[et.al] -- Nitrous oxide emission and global changes : modeling approaches / Lars Bakken, Peter Dörsch -- Interactions among organisms that result in enhanced activities of N-cycle reactions / Hermann Bothe, Harold Drake.
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  • 2
    Online Resource
    Online Resource
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Keywords: Nitrogen-fixing bacteria-Congresses. ; Electronic books.
    Description / Table of Contents: Proceedings of the NATO Advanced Research Workshop Biological Fixation of Nitrogen for Ecology and Substainable Agriculture", held in Poznan, Poland, September 10-14, 1996.
    Type of Medium: Online Resource
    Pages: 1 online resource (321 pages)
    Edition: 1st ed.
    ISBN: 9783642591129
    Series Statement: Nato asi Subseries G: Series ; v.39
    DDC: 572.545293
    Language: English
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  • 3
    Online Resource
    Online Resource
    San Diego :Elsevier Science & Technology,
    Keywords: Nitrogen cycle. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (453 pages)
    Edition: 1st ed.
    ISBN: 9780080471334
    DDC: 577.145
    Language: English
    Note: Front Cover -- Title page -- Copyright page -- Table of Contents -- List of Contributors -- Preface -- Part I: Denitrification -- Chapter 1. Introduction to the Biochemistry and Molecular Biology of Denitrification -- 1.1 Introduction -- 1.2 Proteins of denitrification -- 1.3 Bioenergetics of denitrification -- 1.4 Genes coding for enzymes of denitrification -- 1.5 Regulation of transcription of the denitrification genes -- 1.6 Regulatory networks in denitrifiers -- 1.7 Concluding remarks -- References -- Chapter 2. The Prokaryotic Nitrate Reductases -- 2.1 Introduction -- 2.2 The membrane-bound NO3¯ -reductase (NarGHI) -- 2.3 The Archaeal Nar system -- 2.4 The periplasmic nitrate reductase (Nap) -- 2.5 The assimilatory nitrate reductase (Nas) -- 2.6 The eukaryotic nitrate reductases -- References -- Chapter 3. Nitrite Reductases in Denitrification -- 3.1 Introduction -- 3.2 Cd1 nitrite reductase -- 3.3 Copper nitrite reductase -- References -- Chapter 4. Nitric Oxide Reductase: Structural Variations and Catalytic Mechanism -- 4.1 Introduction -- 4.2 Structural variations in NORs -- 4.3 Catalytic mechanism of NOR -- 4.4 Comparison between catalysis by NOR and CcO -- References -- Chapter 5. Nitrous Oxide Reductases -- 5.1 Introduction -- 5.2 Properties of N2O reductase -- 5.3 Enzyme structure and Cu centers -- 5.4 Metal center assembly -- 5.5 Gene patterns and dissemination of nos genes -- 5.6 Evolutionary aspects -- 5.7 Transport processes for Nos proteins -- 5.8 Role of accessory flavoproteins -- References -- Chapter 6. Denitrification in Rhizobia-Legume Symbiosis -- 6.1 Introduction -- 6.2 Denitrification in free-living rhizobia -- 6.3 Denitrification in nodules -- References -- Chapter 7. The Dissimilatory Reduction of Nitrate to Ammonia by Anaerobic Bacteria. , 7.1 Dissimilatory nitrate reduction to ammonia, a process distinct from denitrification and nitrate assimilation -- 7.2 The cytoplasmic pathway for nitrate dissimilation to ammonia -- 7.3 The periplasmic pathway for respiratory reduction of nitrate to ammonia -- 7.4 Regulation of the cytoplasmic and periplasmic pathways for nitrate reduction to ammonia -- 7.5 Distribution of enzymes for nitrate reduction to ammonia and their ecological significance -- 7.6 Nitrate reduction to ammonia as an evolutionary link between nitrate assimilation and denitrification -- 7.7 Current challenges and unanswered questions -- References -- Part II: Biological Nitrogen Fixation -- Chapter 8. Physiology, Biochemistry, and Molecular Biology of Nitrogen Fixation -- 8.1 Which organisms fix N2? -- 8.2 Nitrogenases -- 8.3 MgATP and Mo-nitrogenase catalysis -- 8.4 Genetics of N2-fixation -- 8.5 Regulation of N2-fixation -- 8.6 Perspectives and future research -- References -- Chapter 9. Regulatory Cascades to Express Nitrogenases -- 9.1. Introduction -- 9.2 Environmental signals regulating expression of nitrogenases -- 9.3 Levels of regulation of N2-fixation in proteobacteria and the central role of PII -- 9.4 Transcriptional control of the nifA gene represents the first level of the regulatory cascade in diazotrophic proteobacteria -- 9.5 NifA activates nif gene expression in concert with a specific sigma factor, RpoN -- 9.6 Control of NifA activity represents the second level of the regulatory cascade -- 9.7 Control of nitrogenase activity represents the third level of the regulatory cascade -- 9.8 Regulation of alternative nitrogenases -- 9.9 Regulation of N2-fixation in nonproteobacterial species -- 9.10 Concluding remarks -- References -- Chapter 10. The Rhizobium-Legume Nitrogen-Fixing Symbiosis -- 10.1 Introduction -- 10.2 An overview of nodule formation. , 10.3 Molecular mechanisms of signal exchange -- 10.4 Symbiotic nitrogen fixation and assimilation -- 10.5 Conclusions -- References -- Chapter 11. Plant Symbioses with Frankia and Cyanobacteria -- 11.1 Introduction -- 11.2 Actinorhizal symbioses -- 11.3 Cyanobacterial symbioses -- References -- Chapter 12. Associative Nitrogen Fixation -- 12.1 Introduction -- 12.2 Non-symbiotic colonization of plants -- 12.3 N2-fixing bacteria associated with plants -- 12.4 Conclusion -- References -- Chapter 13. Measuring N2 Fixation in the Field -- 13.1 Introduction -- 13.2 Rate measurements -- 13.3 Detecting N2 fixation potential and N2-fixing microorganisms -- References -- Part III: Other Reactions of the Nitrogen Cycle -- Chapter 14. Biochemistry and Molecular Biology of Nitrification -- 14.1 Introduction -- 14.2 The general energetic problem faced by lithotrophic nitrifiers -- 14.3 Denitrification reactions catalysed by nitrifiers -- 14.4 Heterotrophic nitrification -- References -- Chapter 15. The Ecology of Nitrifying Bacteria -- 15.1 Introduction -- 15.2 Nitrifying microorganisms -- 15.3 Community structure of nitrifiers in natural environments -- 15.4 Factors influencing the ecology of nitrifying bacteria -- 15.5 Conclusions -- References -- Chapter 16: Anammox -- 16.1 Anammox: Discovery and introduction -- 16.2 Enrichment of anammox biomass -- 16.3 Physiology of anammox aggregates from the SBR -- 16.4 Identification of the key player -- 16.5 Ultrastructure of Brocadia anammoxidans -- 16.6 Lipids of anammox bacteria -- 16.7 Significance of anammox on a global scale -- 16.8 Biochemistry and the role of environmental genomics -- 16.9 The application of the anammox process -- 16.10 Integration and perspectives -- References -- Chapter 17. Nitrate Assimilation in Bacteria -- 17.1 Introduction -- 17.2 The NO3 ¯ and NO2 ¯ uptake systems -- 17.3 Assimilatory NRs. , 17.4 Assimilatory NiRs -- 17.5 Regulation of NO3 ¯ assimilation -- 17.6 Concluding remarks -- References -- Chapter 18. Nitrate Assimilation in Plants -- 18.1 Introduction -- 18.2 Physiology and molecular biology of nitrate uptake -- 18.3 Assimilatory NO3 ¯ reduction in higher plants -- References -- Chapter 19. Characterization of Proteolytic Microbes and Their Activities in Soils -- 19.1 Introduction -- 19.2 Methods to assess proteolysis -- 19.3 Tools to study the gene- and transcript pool of proteolytic organisms -- 19.4 Investigation of proteolysis in terrestrial ecosystems -- References -- Part IV: Applications of Reactions of the Nitrogen Cycle, with Emphasis on Denitrification -- Chapter 20. Molecular Tools to Assess the Diversity and Density of Denitrifying Bacteria in Their Habitats -- 20.1 Introduction -- 20.2 Molecular markers for denitrifying bacteria -- 20.3 Genetic fingerprinting of denitrifier communities -- 20.4 Quantification of denitrifier communities -- 20.5 Examples of denitrifier communities in habitats -- 20.6 Improving our ways to study denitrifiers in habitats -- References -- Chapter 21. Denitrification and Agriculture -- 21.1 Nitrogen in agricultural systems -- 21.2 Factors controlling denitrification in agricultural soils -- 21.3 Agricultural consequences -- 21.4 Environmental consequences of denitrification -- 21.5 Quantification of denitrification losses -- 21.6 Mitigation of denitrification activity in the field -- References -- Chapter 22. Denitrification and N-Cycling in Forest Ecosystems -- 22.1 Introduction -- 22.2 Characteristics of the N-cycle in forest ecosystems -- 22.3 Impact of increased nitrogen inputs on denitrification -- 22.4 Nitrogen inputs affect forest microbial communities -- 22.5 Environmental regulation of denitrification -- 22.6 Species involved in denitrification. , 22.7 Importance of denitrification for N2O-emissions -- 22.8 Effect of forest type on denitrification -- 22.9 Competition between plants and microorganisms -- 22.10 The N2O:N2 ratio and in situ quantification -- 22.11 Modelling -- 22.12 Austrian case - the missing nitrogen -- References -- Chapter 23. Denitrification in Wetlands -- 23.1 Introduction -- 23.2 Wetlands as an environment for denitrification -- 23.3 Molecular diversity of denitrifying bacteria -- 23.4 Denitrification in riparian wetlands -- 23.5 Denitrification in constructed wetlands -- 23.6 Emission of nitrogen gases from wetlands -- References -- Chapter 24. Organisms of the Nitrogen Cycle Under Extreme Conditions: Low Temperature, Salinity, pH Value and Water Stress -- 24.1 Introduction -- 24.2 Low temperature -- 24.3 Denitrification in halophilic environments -- References -- Chapter 25. Nitrous Oxide Emission and Global Changes: Modeling Approaches -- 25.1 Introduction -- 25.2 Product stoichiometry of denitrification -- 25.3 Models of soil anaerobiosis as a regulator for denitrification -- 25.4 Denitrification and N2O flux in soil biogeochemical models -- 25.5 Microbial kinetics of denitrification in biogeochemical models -- References -- Chapter 26. Interactions among Organisms that Result in Enhanced Activities of N-Cycle Reactions -- 26.1 Introductory statement -- 26.2 Associative bacteria are potentially beneficial to the health of plants -- 26.3 Plants eating animals (carnivorous plants) to get access to an extra N-source -- 26.4 The role of mycorrhizal fungi in mobilizing soil nutrients, in particular nitrogen -- 26.5 The earthworm gut as a transient habitat for terrestrial denitrifiers -- References -- Index -- Colour Plate Section.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science, Ltd
    Physiologia plantarum 115 (2002), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: PCR amplifications using tomato DNA and degenerate oligonucleotide primers allowed identification of a new putative nitrate transporter, termed NRT2;3. Its sequence showed typical motifs of a high affinity nitrate transporter of the Major Facilitator Superfamily (MFS). The formation of its mRNA was positively controlled by nitrate, and negatively by ammonium, but not by glutamine. In situ hybridization experiments showed that this transporter was mainly expressed in rhizodermal cells. Results from expression studies with two other nitrate transporters, LeNRT1;1 and LeNRT2;1, were essentially in accord with data of the literature. In roots colonized by the arbuscular mycorrhizal fungus Glomus intraradices Sy167, transcript formation of NRT2;3 extended to the inner cortical cells where the fungal structures, arbuscules and vesicles, were concentrated. Northern analyses indicated that the expression of only NRT2;3 among the transporters assayed was higher in AMF colonized tomato roots than in non-colonized controls. AMF-colonization caused a significant expression of a nitrate reductase gene of G. intraradices. The results may mean that AMF-colonization positively affects nitrate uptake from soil and nitrate allocation to the plant partner, probably mediated preferentially by LeNRT2;3. In addition, part of the nitrate taken up is reduced by the fungal partner itself and may then be transferred, when in excess, as glutamine to the plant symbiotic partner.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Hydrogen metabolism is of central interest in cyanobacterial research because of its potential applications. The gene expression and physiological role of the cyanobacterial bidirectional NAD(P)+-reducing hydrogenase are poorly understood. Transcription rates of hoxEF and hoxUYH encoding this enzyme have been studied in Synechococcus sp. PCC7942. PhoxU activity was about three times higher than that of PhoxE. Circadian phasing of both promoters was found to be synchronous and influenced expression levels by at least one order of magnitude. This is the first demonstration of circadian control of gene expression for any hydrogenase. For the majority of PhoxU-driven messages, transcription presumably terminates between hoxU and hoxH. Being part of a polycistronic hoxUYHW… operon, hoxW, encoding a protease involved in C-terminal processing of the hydrogenase large-subunit HoxH, is mainly expressed by its own promoter, PhoxW. The complex transcript formation may be a key feature for controlling bidirectional hydrogenase expression in vivo.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 195 (2001), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Pyruvate:ferredoxin (flavodoxin) oxidoreductase (PFO, EC 1.2.7.1) catalyses the oxidative cleavage of pyruvate and coenzyme A to acetylcoenzyme A and CO2 with the simultaneous reduction of ferredoxin or flavodoxin. PFO occurs in anaerobes and in some aerobic archaea and bacteria. For cyanobacteria, activity measurements indicated the occurrence of PFO in heterocystous forms. The completely sequenced genomes of the unicellular Synechocystis sp. PCC 6803 and the heterocystous Anabaena sp. PCC 7120 and Nostoc punctiforme revealed the existence of one PFO (encoded by nifJ) in Synechocystis 6803 and N. punctiforme but two different PFOs, encoded by nifJ1 and nifJ2, in Anabaena. Sequence comparison now indicates that all cyanobacterial PFOs are more closely related to those of anaerobes than to those of aerobes. Reverse transcription-polymerase chain reaction (RT-PCR) experiments show that nifJ is transcribed in the presence of saturating iron concentrations in aerobically grown cells of the unicellular Synechococcus sp. PCC 6301 and Synechocystis 6803. Both nifJ genes are transcribed in aerobically grown Anabaena 7120. These findings are corroborated by luciferase reporter gene analysis of nifJ in Synechococcus sp. PCC 7942. The occurrence of PFO in these cyanobacteria is enigmatic.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 24 (2000), S. 0 
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: This review summarizes aspects of the current knowledge about the ecology of ammonia-oxidizing and denitrifying bacteria. The development of molecular techniques has contributed enormously to the rapid recent progress in the field. Different techniques for doing so are discussed. The characterization of ammonia-oxidizing and -denitrifying bacteria by sequencing the genes encoding 16S rRNA and functional proteins opened the possibility of constructing specific probes. It is now possible to monitor the occurrence of a particular species of these bacteria in any habitat and to get an estimate of the relative abundance of different types, even if they are not culturable as yet. These data indicate that the composition of nitrifying and denitrifying communities is complex and apparently subject to large fluctuations, both in time and in space. More attempts are needed to enrich and isolate those bacteria which dominate the processes, and to characterize them by a combination of physiological, biochemical and molecular techniques. While PCR and probing with nucleotides or antibodies are primarily used to study the structure of nitrifying and denitrifying communities, studies of their function in natural habitats, which require quantification at the transcriptional level, are currently not possible.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The diaphorase subunits Hox(E)FU of the cyanobacterial bidirectional hydrogenase complex have been suggested to serve also as the three missing proteins of the cyanobacterial respiratory complex I. These subunits, encoded by nuoEFG in Escherichia coli, contain the NAD+ and FMN binding sites. Previous physiological and molecular experiments demonstrated that neither the bidirectional hydrogenase activity nor hoxYH, encoding the hydrogenase dimer of the bidirectional enzyme, occur in the heterocystous cyanobacterium Nostoc PCC 73102. The present study demonstrates, by heterologous Southern blot hybridizations, that the genes hoxFU, encoding diaphorase subunits of the bidirectional enzyme, are both not present in Nostoc PCC 73102, whilst the genes hoxFU were detectable in all other heterocystous and unicellular cyanobacteria examined which possess the bidirectional hydrogenase. However, Nostoc PCC 73102 respires with rates comparable to those of other cyanobacteria and sequences similar to the genes ndhJ, ndhD2, ndhA and ndhI, encoding subunits of the respiratory complex I of Synechocystis PCC 6803, are present within the genome of Nostoc PCC 73102. Previous studies, using the unicellular strains Anacystis nidulans and Synechocystis PCC 6803, demonstrated that mutants in the diaphorase genes hoxU or hoxF are unable to evolve H2, whereas the respiration is not affected. Altogether, these data are strongly against the hypothesis of a common use of the hox(E)FU gene products by the bidirectional hydrogenase and by the respiratory complex I in cyanobacteria.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Twenty-six Hyphomicrobium isolates from the sewage treatment plant and its receiving water body in Plön (Schleswig-Holstein, Germany) and two culture collection strains were screened for the occurrence of genes coding for denitrification enzymes (dissimilatory nitrate, nitrite and nitrous oxide reductases), for dinitrogen fixation (nitrogenase reductase) and for nitrification (ammonia monooxygenase catalyzing the first stage of this process) by DNA-probing. More than one half of the isolates had genes coding for denitrification enzymes. The DNA-DNA hybridization signals obtained with the gene segments correlated with enzyme activity measurements. The DNA of some isolates distinctly hybridized with the nifH probe indicating the occurrence of nitrogenase in the genus Hyphomicrobium. No signal was detected with the gene probe for nitrification. The results show that probes consisting of gene segments can be employed successfully to monitor the occurrence of genes which can show complex expression and in bacteria growing at low rates. The distribution pattern of the denitrification genes indicates that methylotrophic prosthecate bacteria of the sewage treatment plant and its receiving water body occupy different ecological niches.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 36 (2001), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The populations of N2-fixing and denitrifying bacteria in an acid forest soil near Cologne were characterized by gene probing. The DNA isolated from the soil for this purpose was suitable for DNA–DNA hybridization using 0.4–0.7-kb probes targeting denitrification enzymes, dinitrogenase reductase (nifH) and eubacterial 16S rRNA. The densitometrical comparison of band intensities obtained in these Southern hybridizations indicated that the highest number of total bacteria, of denitrifying and N2-fixing microorganisms always occurred in the upper (∼5 cm) soil layer. The concentration of all these organisms decreased in parallel with the soil depth. The soil investigated was rich in nitrate in all layers, and the availability of nitrate apparently did not govern the distribution of denitrifying and N2-fixing bacteria in this soil. Soil cores investigated in the laboratory formed N2O on addition of nitrate irrespective of the presence of C2H2. Hybridization intensities, with a gene probe for the 16S rRNA, and MPN numbers were generally higher in soil samples taken from the roots of plants than in the bulk soil. There was no selective enrichment of denitrifying or N2-fixing bacteria at the roots. The data obtained by hybridizing isolated soil DNA generally matched previous results obtained with culturable bacteria.
    Type of Medium: Electronic Resource
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