Note: Front Cover -- Transgenesis and Targeted Mutagenesis in Immunology -- Copyright Page -- Table of Contents -- Contributors -- Preface -- Chapter 1. Transgenic Mouse Models to Study VDJ Recombination -- I. Introduction -- II. Transgenic Model to Define VDJ Recombination Regulatory Elements: Evidence That Recombinational Sequences Are Associated with Lymphoid Transcriptional Enhancers -- III. Other Transgenic Models -- IV. Future Prospects -- V. Concluding Remarks -- Acknowledgments -- References -- Chapter 2. Lymphocyte Development and Function in T Cell Receptor and RAG-1Mutant Mice -- I. Introduction -- II. Creation of Mutant Mice -- III. Thymocyte Development -- IV. Model for the Role of TCR Genes in αß Thymocyte Differentiation -- V. Lymphocyte Function -- Acknowledgments -- References -- Chapter 3. RAG-2-Deficient Blastocyst Complementation -- I. Introduction -- II. Procedures -- III. Applications -- IV. Prospects -- Acknowledgments -- References -- Chapter 4. Role of CD4 and CD8 in Determining Cell Fate in the Thymus -- I. Background -- II. Coreceptor Transgenes and TCR Repertoire Selection -- III. Coreceptor Transgenes and Thymocyte Lineage Commitment -- IV. Other Data Pertaining to Coreceptor Lineage Commitment -- V. Future Directions -- Acknowledgments -- References -- Chapter 5. T Cell Development in CD4, CD8, and p56ICK Gene-Targeted Mice -- I. Introduction -- II. T Cell Development in CD4, CD8, and CD4 and CD8 Double-Negative Mice -- III. Thymic Development in p56ICK-Deficient Mice -- IV. Concluding Remarks -- References -- Chapter 6. T Cell Tolerance in T Cell Receptor Transgenic Ipr/lpr Mice -- I. Introduction -- II. Effect of T Cell Receptor Transgenes on T Cell Development in Ipr/lpr Mice -- III. Loss of T Cell Tolerance in Db/HY T Cell Receptor Transgenic Ipr/lpr Mice. , IV. Loss of T Cell Tolerance in Vβ8 T Cell Receptor Transgenic MRL Ipr/lpr Mice Neonatally Tolerized by the Staphylococcal Enterotoxin B -- V. Costimulatory Signaling in Vβ8 T Cell Receptor Transgenic MRL Ipr/lpr Mice Neonatally Tolerized with Staphylococcal Enterotoxin B -- VI. Summary -- Acknowledgments -- References -- Chapter 7. Thymocyte Selection and Peripheral Tolerance Using the Lymphocytic Choriomeningitis Virus as a Model Antigen -- I. Introduction -- II. Positive Selection -- III. Negative Selection -- IV. Tolerance to Peripheral Self-Antigens -- V. Factors That Influence the Induction of Self-Reactivity -- VI. Prevention of Autoimmune Disease -- VII. Concluding Remarks -- Acknowledgments -- References -- Chapter 8. Peripheral T Cell Tolerance: Distinct Levels and Multistep Mechanisms -- I. Introduction -- II. Thymic Tolerance Induction by Minute Amounts of Antigen -- III. Parameters Controlling Peripheral Tolerance Induction -- IV. Distinct Manifestations of Peripheral Tolerance Due to Differential Expression of Self-Antigen -- V. Influence of the Amount of Antigen Expressed on Peripheral Tolerance Induction -- VI. Tolerance Induction as a Multistep Process -- VII. Anergy versus Memory -- VIII. Summary and Conclusions -- References -- Chapter 9. Peripheral Tolerance and CD4+ T Cells -- I. Introduction -- II. Class II MHC Transgenic Animals -- III. INS-HA Transgenic Animals -- IV. TCR INS-HA Transgenic Animals -- V. Discussion -- VI. Conclusions -- Acknowledgments -- References -- Chapter 10. Studies of Tolerance, Inflammation, and Autoimmunity inTransgenic Mice -- I. Introduction -- II. TNFα and TNFβ Are Sufficient to Induce an Inflammatory Infiltrate but Cannot Induce Autoimmunity -- III. Antigen Recognition and Its Consequences in SV-40 T Antigen Transgenic Mice. , IV. Two-Signal Model of T Cell Activation: Role of T Cell Activation in the Induction of Autoimmunity -- V. Can Locally Produced Interleukin 2 Bypass Tolerance Mechanisms?: Interleukin 2 Expression in Pancreatic B Cells Leads to Insulitis and Diabetes -- VI. Concluding Remarks -- Acknowledgments -- References -- Chapter 11. Immunological Studies Utilizing Cytokine Transgenic Mice -- I. Introduction -- II. Localized Expression of Interferon y -- III. Expression of Interleukin 10, an Immunoinhibitory Cytokine -- IV. Expression of Other Cytokines -- V. Expression of Molecules Other Than Cytokines -- VI. Conclusion -- References -- Chapter 12. Effect of MHC Class II Encoding Transgenes on Autoimmunity in Nonobese Diabetic Mice -- I. Introduction -- II. Effect of Transgenic Ea Expression on Insulin-Dependent Diabetes Mellitus -- III. Effect of Mutated Ab Transgenes on Insulin-Dependent Diabetes Mellitus -- IV. Conclusion -- References -- Chapter 13. Development and Function of the Immune System in Mice with Targeted Disruption of the Interleukin 2 Gene -- I. Introduction -- II. Generation of Interleukin 4-Deficient Mice by Targeted Mutagenesis -- III. Effect of Interleukin 4 on B Cell Development and Differentiation -- IV. Effect of Interleukin 4 on T Cell Development and Differentiation -- V. Role of Interleukin 4 in Allergy -- VI. Outlook -- Acknowledgments -- References -- Chapter 14. Immune Responses in Interleukin 4-Deficient Mice -- I. Introduction -- II. Generation of Interleukin 2-Deficient Mice -- III. Lymphopoiesis and Leukocyte Homeostasis in Young and Adult Mutant Mice -- IV. Immune Functions in Young Healthy Animals -- V. Development of Disease and Mortality of Adult Interleukin 2-Deficient Mice -- VI. Inflammatory Bowel Disease in Interleukin 2-Deficient Mice -- VII. Concluding Remarks -- References. , Chapter 15. Manipulation of Transgene-Encoded Self-Antigens to Explore Mechanisms of B Cell Tolerance -- I. Introduction -- II. Anti-CD8 IgM Transgenic Mouse Lines -- III. Conclusions -- Acknowledgments -- References -- Chapter 16. Transgenic Mouse Model for Peripheral B Cell Tolerance -- I. Introduction -- II. Effects of Lysozyme on Self-Reactive B Cells -- III. B Cell Anergy versus Elimination -- IV. Physiological and Pathological Implications of B Cell Elimination by Arrested Development -- References -- Chapter 17. Immunobiology of MHC Class l-Deficient Mice -- I. Introduction -- II. Production of ß2m-Deficient Mice -- III. Effects of ß2m Deficiency on Cell Surface Expression of Class I Molecules -- IV. Defective Lymphocyte Development in Class l-Deficient Mice -- V. Effect of Class I Deficiency on Susceptibility to Natural Killer Cells and Development of Natural Killer Activity -- VI. Transplantation Studies -- VII. Responses of Class l-Deficient Mice to Pathogens -- VIII. Summary and Conclusions -- Acknowledgments -- References -- Chapter 18. MHC Class II Mutant Mice -- I. Introduction -- II. Studies in MHC Class II Mutant Mice -- III. MHC-Deficient Mice -- IV. Conclusion -- Acknowledgments -- References -- Index.