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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Clinica Chimica Acta 76 (1977), S. 183-186 
    ISSN: 0009-8981
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Interleukin 2(IL-2) and gamma interferon (IFN-γ) were determined in supernatantsof mitogen and antigen-driven cell cultures from patients with hepatosplenic or intestinal schistosomiasis. Skin reactivity was tested using a panel of eight recall antigens. Results were compared with those of uninfected local controls. In both schistosomiasis groups, IL-2 activity was reduced before treatment. In less than one third of the patients, schistosomal antigens elicited detectable IL-2 activity. IFN-γ production was reduced more severely in hapatosplenic cases, in particular after stimulation by anti-CD3 monoclonal antibodies. After anti-schistosomal therapy with praziquantel, mitogen-induced IL-2 and IFN-γ activities became normal within 3 months in intestinal schistosomiasis, and within 6 months in the hepatosplenic patient group. Results of in vivo delayed-type hypersensitivity tests paralleled those of in vitro lymphokine production. In conclusion, evidence is presented for severe, antigen-unspecific suppression of lymphokine production and skin reactivity against recall antigens. Anti-parasitic chemotherapy is shown to reverse the impairment of cell-mediated immune responses at the cytokine level.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 102 (1989), S. 487-493 
    ISSN: 1437-1596
    Keywords: HIV-antibodies in bloodstains ; HIV-Antikörper in Blutspuren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wurden Blutspuren auf Baumwolltextil asserviert und bei Raumtemperatur gelagert. Diese Proben wurden im HIV-ELISA und -Western Blot untersucht. Dabei gelang ein HIV-Antikörpernachweis an bis zu vier Monate alten Proben.
    Notes: Summary Blood samples were collected on cotton wool and stored at +20°C. These samples were tested in an enzyme linked immunosorbent assay and the immunoblotting test. HIV-antibodies could be detected in samples stored up to four month.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1440
    Keywords: AIDS ; Lymphadenopathy ; Prognostic factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 26 homosexual men with antibodies to HIV and generalized lymphadenopathy were examined between 1982 and 1984. Lymphadenopathy was accompanied by clinical symptoms such as fever, diarrhoea and weight loss (50%), moderate leukopenia (15%), lymphocytosis (50%), hypoergy to intraepidermal skin test with recall-antigens (12%), elevated serum levels of IgG (62%), IgA (23%), IgM (19%), beta-2-microglobulin (76%) and neopterin (69%), diminished absolute numbers of CD4-helper cells in the peripheral blood (24%) and an inversion of the ratio CD4-helper cells/CD8-suppressor cells both in blood (64%) and in lymphnodes (36%). Mitogen induced lymphocyte proliferation was significantly lower and the serum levels of gamma-interferon were significantly higher than in healthy controls. Lymphnode biopsy revealed only nonspecific reactive hyperplasia with follicular hyperplasia in 54%, a mixed pattern of both follicular hyperplasia and follicular involution with paracortical expansion in 25% and follicular involution with paracortical expansion in 21%. 22 patients were followed longitudinally for a median time of 37 months (33–51 months). 10 patients (46%) developed fullblown AIDS within a median period of 35 months (24–46 months) after first examination and 43 months (36–77 months) after the anamnestic onset of the lymphadenopathy syndrome. The parameters distinguishing these patients from those without progression of disease were higher serum levels of gamma-interferon, higher relative and absolute numbers of CD8-suppressor cells in the peripheral blood, a reduced number of CD4-helper cells in the lymphnodes, and a lower CD4/CD8 ratio as well in the peripheral blood as in the lymphnodes. Despite of the significant differences, these parameters varied in a wide range. Therefore, we could not establish a symptom likely to predict the emergence of AIDS in an individual patient.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1440
    Keywords: AIDS ; Lymphadenopathy ; Prognostic factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 26 homosexual men with HIV-infection and lymphadenopathy syndrome were examined between 1982 and 1984 and prospectivelly observed over a period of 58 months (median, range 53–69 months). 16 (61.5%) developed full blown AIDS within a median period of 41 months (23–63 months). 12 of them died 50 months (median, range 29–67 months) after the first examination. 10 patients showed no symptoms of AIDS after a median observation time of 57 months (54–63 months). A panel of clinical symptoms, signs of concomitant infections, chemical and immunological analyses, including a lymphnode biopsy were correlated to the course of the disease. A decreased proportion of CD4-cells and a lower CD4/CD8ratio in the lymphnode suspensions at the first examination in patients with disease progression were the only parameter which correlated significantly with the development of AIDS.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 65 (1987), S. 376-379 
    ISSN: 1432-1440
    Keywords: Drug addicts ; HIV antibodies ; Risk factors ; Seroepidemiological features
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A series of 320 German i.v. drug abusers (32.2% female, 67.8% male) were tested in a cross-sectional study for antibodies against HIV. Seroprevalence increased from 0 in those who discontinued i.v. drug abuse before 1982 to 37.2% in those who stopped injecting drugs in 1985/1986 or who were still addicted. Antibodies to HIV were significantly associated with lymphadenopathy and clinical symptoms (fever, weight loss, diarrhea, fatigue, night sweat, dermal lesions) and with markers of hepatitis A and hepatitis B virus infection. Participants of the study admitted in 92.5% of cases to “needle sharing” and in 83.1% of cases to sexual contacts among drug abusers. Prostitution and drug abuse in prison were significantly correlated with seropositivity. No antibodies to HIV infection were detected in 131 subjects of a control group of household contacts.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 68 (1990), S. 1082-1082 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1440
    Keywords: Homosexuals ; HIV infection ; Risk factors ; Seroepidemiological characteristics ; Clinical findings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sera of 1980 homo- and bisexual men who visited the Landesinstitut für Tropenmedizin Berlin (West) between April 1983 and December 1987 were tested for HIV antibodies; 24.3% were positive. Of HIV-antibody-positive men, 78.9% reported both active and passive anal intercourse, 58.8%, rectal enemas, and 53.3%, use of butyl nitrite. There was a sexual partner with known positive HIV-antibody status or AIDS in 33.9%, and in 32.4% there was a history of sexual activity in the USA. In the year before the test, 18.8% had had more than 50 partners. All these behavioral characteristics were found to be significantly correlated to HIV antibodies. We noted a substantial reduction of high-risk behavior from 1983 to 1987. The most important behavioral factor for HIV infection in 1983 and 1984 was sexual activity in the USA, and from 1984 to 1987, the numbers of lifetime partners. Persons infected with HIV were significantly more often carriers of antibodies against HAV, HBV, CMV, EBV, and syphilis. Prevalence of antibodies against HIV, HAV, HBV, and syphilis increased with age, duration of homosexual practice, and the number of partners. Overall crude prevalence rates of HIV antibodies, anti-HBc, anti-HAV, and antibodies to syphilis declined during the observation period. Clinical findings such as fever, oral lesions, and lymphadenopathy syndrome (LAS) were found to be highly indicative of HIV infection. Lower hemoglobin values, a reduced white cell count, and hyperimmunoglobulinemia were significantly more frequent in subjects with HIV antibodies.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1440
    Keywords: Intravenous drug abusers ; HIV infection ; Risk factors ; Seroepidemiological features ; Time trends
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To investigate the epidemiology of HIV infection among intravenous drug abusers (IVDA) in Berlin (West), from October 1984 to October 1988, 741 heterosexual IVDA were recruited from facilities for drug treatment and counseling. In this group 22.8% were seropositive for antibodies against HIV, representing 21.1% of the females and 23.5% of the males. Seroprevalences of hepatitis B, hepatitis A, and lues were 67.7%, 40.1%, and 4.0%, respectively. Seropositivity for HIV antibodies correlated with positive seromarkers for hepatitis B and A, and with certain behavioral and social features such as sharing of injection equipment, imprisonment, and intravenous drug use in prison. The crude time trend of HIV seroprevalence shows an increase from 17.1% in those subjects who discontinued i.v. drug abuse in 1983 or earlier to 31.5% in 1985, and a decrease over the past 3 years to 14.1% in 1988. After adjusting for temporary changes in the study group, the estimated HIV seroprevalence odds show an almost steady increase and were significantly higher for those who were injecting drugs in 1987 and 1988 compared with those who stopped i.v. drug use before 1984. Thus IVDA with a persistent risk profile are at a still-increasing risk of acquiring HIV infection.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 42 (1981), S. 157-164 
    ISSN: 1432-0584
    Keywords: Malaria ; Thrombozytopenie ; Thrombozytenlebenszeit ; Malaria ; Thrombocytopenia ; Platelet survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Platelet counts were investigated in 26 patients withP. falciparum malaria and 39 patients withP. vivax malaria before and after treatment. Before schizontocidal treatment 22 of 26 (85%) patients withP. falciparum malaria and 30 of 39 (72%) patients withP. vivax malaria had depressed platelet counts below 150,000/Μl blood. There was a correlation between low platelet counts and high counts of malarial plasmodia (parasitized red blood cells) inP. falciparum andP. vivax infections (p 〈 0.001). Platelet survival, studied by malonaldehyde formation in three patients during the period of decreasing parasitaemia, revealed a shortened life span to 2–3 days in comparison to 7–10 days in normal controls. In all patients platelet counts rose to threefold the initial values within 5 days after clearance of parasites. The results demonstrate that, first, thrombocytopenia is a common feature in human malaria, second, thrombocytopenia induced by malaria is due to shortened life span in the peripheral blood and, third, some interaction is present between platelets and malaria plasmodia or parasitized red cells.
    Notes: Zusammenfassung Bei 26 Patienten mitMalaria tropica und 39 Patienten mitMalaria tertiana wurden die Thrombozytenzahlen vor und nach Behandlung untersucht. Vor der spezifischen Malariabehandlung zeigten 22 von 26 Patienten mitMalaria tropica (85%) und 30 von 39 Patienten mitMalaria tertiana (72%) erniedrigte Plättchenzahlen unter 150 000/Μd Blut. Zwischen niedrigen Thrombozytenzahlen und hoher Parasitämie bestand eine eindeutige Korrelation sowohl bei Infektionen durchP. falciparum als auch durchP. vivax (p 〈 0,001). Bei drei Patienten wurde während der abklingenden Parasitämie die Lebenszeit der Thrombozyten mit der Methode der Malonaldehyd-Bildung untersucht. Sie zeigte eine auf 2–3 Tage verkürzte Lebenszeit der Thrombozyten im Vergleich zu 7–10 Tagen bei normalen Kontrollpersonen. In allen Patienten stiegen die Thrombozytenzahlen nach beendeter malariaspezifischer Behandlung innerhalb von 5 Tagen bis auf das Dreifache der Ausgangswerte an. Die Ergebnisse lassen darauf schlie\en, da\ erstens eine Thrombozytopenie regelmä\ig bei der menschlichen Malaria vorhanden ist, zweitens die durch Malaria induzierte Thrombozytopenie bedingt ist durch eine verkürzte Lebenszeit der Thrombozyten im peripheren Blut, und drittens, da\ eine Interaktion zwischen Thrombozyten und den Malariaplasmodien bzw. den parasitierten Erythrozyten besteht.
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