Note: Front Cover -- Isotachophoresis: Theory, Instrumentation and Applications -- Copyright Page -- Contents -- Preface -- Chapter 1. Historical review -- Summary -- 1. Historical review -- References -- PART 1: THEORY -- Chapter 2. Principles of electrophoretic techniques -- Summary -- 2.1. Introduction -- 2.2. Principle of zone electrophoresis -- 2.3. Principle of moving-boundary electrophoresis -- 2.4. Principle of isotachophoresis -- 2.5. Principle of isoelectric focusing -- 2.6. Discussicin -- Chapter 3. Concept of mobility -- Summary -- 3.1. Introduction -- 3.2. Interpretation of electrophoretic migration -- 3.3. Ionic mobility and ionic equivalent conductivity -- 3.4. Effective ionic mobility -- 3.5. Determination of ionic mobilities -- References -- Chapter 4. Mathematical model for isotachophoresis -- Summary -- 4.1. Introduction -- 4.2. General equations -- 4.3. Mathematical model for the steady state in isotachophoresis -- 4.4. Validity of the isotachophoretic model -- 4.5. Check of the isotachophoretic model -- References -- Chapter 5. Choice of electrolyte systems -- Summary -- 5.1. Introduction -- 5.2. Choice of the solvent -- 5.3. Choice of the buffering counter ionic species -- 5.4. Choice of the pH of the leading electrolyte -- 5.5. Choice of the terminating and leading ionic species -- 5.6. Additions to the electrolyte solutions -- 5.7. Discussion -- 5.8. Examples -- References -- PART 2: INSTRUMENTATION -- Chapter 6. Detection systems -- Summary -- 6.1. Introduction -- 6.2. Thermometric recording -- 6.3. High-frequency conductivity detection -- 6.4. Conductivity detection -- 6.5. UV absorption meter -- 6.6. Additives to the electrolytes -- 6.7. Coating of the micro-sensing electrodes -- 6.8. Detection limits -- 6.9. Conclusion -- References -- Chapter 7. Instrumentation -- Summary -- 7.1. Introduction -- 7.2. Injection systems. , 7.3. Counter electrode compartments -- 7.4. Equipment -- 7.5. Counter flow of electrolyte -- PART 3: APPLICATIONS -- Chapter 8. Introduction -- Summary -- 8. Introduction -- Chapter 9. Practical aspects -- Summary -- 9.1. Introduction -- 9.2. Disturbances caused by hydrogen and hydroxyl ions -- 9.3. Disturbances due to the presence of carbon dioxide -- 9.4. Enforced isotachophoresis -- 9.5. Water as terminator -- 9.6. Purification of the terminator -- 9.7. Conversion of data measured with different detectors -- References -- Chapter 10. Quantitative aspects -- Summary -- 10.1. Introduction -- 10.2. Theoretical -- 10.3. Thermometric measurements -- 10.4. Conductimetric measurements -- 10.5. Conclusion -- References -- Chapter 11. Separation of cationic species in aqueous solutions -- Summary -- 11.1. Separation of cationic species in aqueous solutions using a thermocouple as detector -- 11.2. Separation of cationic species in water and deuterium oxide using a conductivity detector (a.c. method) and a UV absorption detector (256 nm) -- Chapter 12 . Separation of anionic species in aqueous solutions -- Summary -- 12.1. Separation of anionic species in aqueous solutions using a thermometric detector -- 12.2. Separation of anionic species in aqueous solutions using a conductivity detector (a.c. method) and a UV absorption detector (256 nm) -- Chapter 13. Amino acids, peptides and proteins -- Summary -- 13.1. Amino acids -- 13.2. Separation of proteins in ampholyte gradients -- 13.3. Separation of small peptides -- References -- Chapter 14. Separation of nucleotides in aqueous systems -- Summary -- 14.1. Introduction -- 14.2. Separation using a thermometric detector -- 14.3. Separation using a conductivity detector (a.c. method) and a UV absorption detector (256 nm) -- Chapter 15. Enzymatic reactions -- Summary -- 15.1. Introduction. , 15.2. Enzymatic conversion of glucose (fructose) into glucose-6-phosphate (fructose-6-phosphate) with hexokinase from yeast -- 15.3. Enzymatic conversion of pyruvate into lactate with lactate dehydrogenase from pig heart -- References -- Chapter 16. Separations in non-aqueous systems -- Summary -- 16.1. Introduction -- 16.2. Separation of anionic species in methanol using a thermometric detector -- 16.3. Separation of cationic species in methanol using a thermometric detector -- 16.4. Experiments in aqueous methanolic systems using a conductimetric detector (a.c. method) and UV absorption detector (256 nm) -- Chapter 17. Counter flow of electrolyte -- Summary -- 17.1. lntroduction -- 17.2. Experimental -- 17.3. Conclusion -- References -- PART 4: APPENDICES -- Appendix A. Simplified model of moving-boundary electrophoresis for the measurement of effective mobilities -- A.1. Introduction -- A.2. Model of moving-boundary electrophoresis -- A.3. Procedure of computation -- A.4. Experimental -- A.5. Discussion -- References -- Appendix B. Diameter of the narrow-bore tube, applied for separation -- Appendix C. Literature -- Symbols and abbreviations -- Symbols -- Subscripts -- Superscripts -- Examples -- Abbreviations -- Subject index.