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  • 1
    Electronic Resource
    Electronic Resource
    Phylogenetic and functional diversity of bacterioplankton during Alexandrium spp. blooms (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 54 (2005), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The phylogenetic and functional diversity of the bacterioplankton assemblage associated with blooms of toxic Alexandrium spp. was studied in three harbours of the NW Mediterranean. Denaturing gradient gel electrophoresis and DNA sequence analysis revealed the presence of a bacterium within the Roseobacter clade related to the presence of Alexandrium cells. Phylogenetic diversity was affected by the presence of Alexandrium spp., geographic situation and seasonality. In contrast, functional diversity, assessed with Biolog plates, was clearly affected by seasonality, but not by the presence of Alexandrium, indicating that the presence of the bacterium associated with the blooms was not enough to modify the metabolic pattern of the bacterioplankton assemblage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2005.04.005
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Picoeukaryotic diversity in an oligotrophic coastal site studied by molecular and culturing approaches (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 50 (2004), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Planktonic picoeukaryotes are important players in coastal marine food webs but little is known about their diversity. Here we report the picoeukaryotic diversity in Blanes Bay (NW Mediterranean) by retrieving environmental 18S rDNA sequences and by obtaining stable cultures. Four genetic libraries (one per season) were constructed and 383 clones analyzed, yielding 176 distinct sequences. The diversity of picoeukaryotes was very large, both at higher and lower phylogenetic levels. Novel alveolates-I (36% of clones), dinoflagellates (17%), novel stramenopiles (10%), prasinophytes (5%) novel alveolates-II (5%), and cryptophytes (4%) were the better represented phylogenetic groups. Nineteen additional groups were found at 〈3% clonal abundance. The four genetic libraries were dominated by the above-mentioned groups, implying a relative stability at high taxonomic level, but identical sequences were seldom found in consecutive dates, suggesting fast temporal changes of picoeukaryotic populations. Coastal and open sea picoeukaryotes were similar, but the representation of groups varied between habitats. The culturing effort revealed that some groups were well represented in clone libraries and in cultures (prasinophytes), others were found by both approaches but often with different sequences (cryptophytes), and others were found only in cultures (bicosoecids) or in clone libraries (novel alveolates and stramenopiles). Our data confirm that molecular approaches, such as cloning and sequencing 18S rRNA genes, are a necessary first step to study picoeukaryotic diversity. These results will aid to focus future research, most likely based on new and imaginative culturing efforts and the design and application of specific molecular probes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2004.07.001
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    Paper (German National Licenses)
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  • 3
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    Seawater carbonate chemistry and microbial abundances, bacterial activity and extracellular enzyme activities (2016)
    PANGAEA
    Details
    Publication Date: 2024-03-18
    Description: We investigated the effects of an increase in dissolved CO2 on the microbial communities of the Mediterranean Sea during two mesocosm experiments in two contrasting seasons: winter, at the peak of the annual phytoplankton bloom, and summer, under low nutrient conditions. The experiments included treatments with acidification and nutrient addition, and combinations of the two. We followed the effects of ocean acidification (OA) on the abundance of the main groups of microorganisms (diatoms, dinoflagellates, nanoeukaryotes, picoeukaryotes, cyanobacteria, and heterotrophic bacteria) and on bacterial activity, leucine incorporation, and extracellular enzyme activity. Our results showed a clear stimulation effect of OA on the abundance of small phytoplankton (pico- and nanoeukaryotes), independently of the season and nutrient availability. A large number of the measured variables showed significant positive effects of acidification in summer compared with winter, when the effects were sometimes negative. Effects of OA were more conspicuous when nutrient concentrations were low. Our results therefore suggest that microbial communities in oligotrophic waters are considerably affected by OA, whereas microbes in more productive waters are less affected. The overall enhancing effect of acidification on eukaryotic pico- and nanophytoplankton, in comparison with the non-significant or even negative response to nutrient-rich conditions of larger groups and autotrophic prokaryotes, suggests a shift towards medium-sized producers in a future acidified ocean.
    Keywords: Abundance per volume; Alkalinity, total; alpha-glucosidase activity; Aragonite saturation state; beta-glucosidase activity; Bicarbonate ion; Biomass/Abundance/Elemental composition; Blanes_Bay_Microbial_Observatory; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chitobiase activity; Chlorophyll a; Coast and continental shelf; Community composition and diversity; Containers and aquaria (20-1000 L or 〈 1 m**2); Entire community; EXP; Experiment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Leucine aminopeptidase activity; Leucine incorporation rate; Macro-nutrients; Mediterranean Sea; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Potentiometric titration; Replicate; Salinity; Spectrophotometric; Temperate; Temperature; Temperature, water; Treatment; Type
    Type: Dataset
    Format: text/tab-separated-values, 3914 data points
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    DATA PANGAEA
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