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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    The @International Journal Of Applied Radiation And Isotopes 15 (1964), S. 502 
    ISSN: 0020-708X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0304-4238
    Keywords: Saintpaulia ionantha ; homogenization ; in vitro ; micropropagation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Plant Science 54 (1988), S. 219-222 
    ISSN: 0168-9452
    Keywords: chromosome doubling ; colhicine ; nitrous oxide ; wheat haploids
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Plant Science 91 (1993), S. 75-86 
    ISSN: 0168-9452
    Keywords: agarose isoelectric focusing ; anther culture ; barley ; embryo specific isozymes
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 102 (1998), S. 101-108 
    ISSN: 1573-5060
    Keywords: albinism ; chromosome doubling ; colchicine treatment ; green plants ; haploids ; microspore embryogenesis ; T. aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Isolated microspores of two DH lines of wheat were treated with 8 different colchicine concentrations up to 3 mM for either 24 h or 48 h during microspore culture. Untreated control cultures produced on average 220 embryos per spike (100,000 microspores), 68% of the regenerated plantlets were green, and 15% of the flowering plants were fertile. The colchicine treatments had a significant effect on chromosome doubling as measured by the percentage of fertile regenerants. Using colchicine concentrations around 1 mM the percentage of fertile plants among the regenerants was increased up to 53%. The highest number of embryos and regeneration rates were observed after 24 h colchicine treatment, while the highest frequencies of green plants and fertile plants were obtained with 48 h colchicine treatments. The highest number of DH plants per spike was found after treatment with colchicine concentrations of 300 to 1000 μM. Such treatments resulted in an estimated average between the two genotypes of 23 doubled haploid plants per spike.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 105 (1999), S. 1-5 
    ISSN: 1573-5060
    Keywords: flower heteromorphism ; heritability ; Primula vulgaris ; seed set ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Within-line pollinations were used when measuring the seed set of plants from seven early generation inbred lines of Primula vulgaris. One high seed setting plant and one low seed setting one were selected from each line and the seed set was evaluated on the offspring from selfings and within-line pollinations of these plants. Regression of offspring on parents for seed set was studied. The slope was estimated to be 0.17 ± 0.08, equivalent to an estimated heritability of around 34%. No significant differences in the slope of regression between the two reproduction methods (selfings or within-line pollinations) were detected. The realized improvement through one cycle of selection of high seed setting single plants in this early generation inbred material of P. vulgaris was 23.7%. Analysis of the seed set of within-line pollinated clones from late generation inbred lines of P. vulgaris revealed that only limited within-line variation remained after removal of the effects of the heteromorphic incompatibility system. It was concluded that selection of high seed setting single plants during early generations of inbreeding is efficient for improvement of seed set in future inbred lines of P. vulgaris to be used for hybrid seed production.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-0662
    Keywords: stratosphere ; ozone loss ; ground-based ; balloon-sonde ; model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The total ozone reduction in the Arctic during the winters of 1993/94 and 1994/95 has been evaluated using the ground-based total ozone measurements of five SAOZ spectrometers distributed in the Arctic and from number density profiles of a balloon-borne version of the instrument. The ozone change resulting from transport has been removed using a 3D Chemistry Transport Model (CTM) run without chemistry. A cumulative total ozone depletion at the end of winter in March of 18% ± 4% in 1994 and of 32% ± 4% in 1995 was observed within the polar vortex, and of 15% ± 4% in both years outside the vortex. This evaluation is not sensitive to the vertical transport in the model. The periods, locations and altitudes at which ozone loss occurred were tightly connected to temperatures lower than NAT condensation temperature. The maximum loss was observed at 50 hPa in 1994 and lower, 60-80 hPa, in 1995. Half of the depletion in 1994 and three quarters in 1995 occurred during the early winter, showing that a late final warming is not a prerequisite for large ozone destruction in the northern hemisphere. The timing, the geographical location and the altitude of the ozone losses are well captured by the 3D CTM photochemical model using current chemistry, but its amplitude at low sun during the early winter, is underestimated. The model simulations also capture the early season reductions observed outside the vortex. This suggests that the losses occurred in situ in the early winter, when low temperatures are frequent, and not later in March, when ozone is most reduced inside the vortex, which would be the case if leakage from the vortex was the cause of the depletion.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5060
    Keywords: Primula vulgaris ; seed set ; diallel ; heteromorphism ; incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Analyses of a 12 × 12 diallel cross among inbred lines of Primula vulgaris disclosed considerable genetic variation for seed set, with differences between floral morphs accounting for 37.3% of the total variation. The differences between morphs agree with those reported elsewhere, with intra-thrum crosses being almost sterile, and intra-pin crosses relatively fertile. Inter-morph crosses produced higher seed yield when the thrum morph was used as the maternal parent. Results further showed that significant genetic effects, explaining 25.2% of the total variation, remained when the effects of the heteromorphic incompatibility system had been removed. Analysis revealed that these effects are due mainly to additive genetic differences between the lines and reciprocal general effects. A factorial analysis of the data indicated that differences in seed set capacity occurred only when the lines were evaluated as females. Seed set from within-line and between-line pollinations were highly positively correlated. These results suggest that selection for within-line fertility during inbreeding of parental lines could be an efficient method for improving seed setting in future inbred lines of P. Vulgaris for hybrid sedd production.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-5060
    Keywords: amiprophos-methyl ; Beta vulgaris ; chromosome doubling ; colchicine ; in vitro culture ; oryzalin ; pronamide ; trifluralin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In vitro chromosome doubling during ovule culture of sugar and fodder beets (Beta vulgaris L.) was studied with four anti-microtubule herbicides: amiprophos-methyl (APM), oryzalin, pronamide, and trifluralin at concentrations of 0–300 μM. Best chromosome doubling results were obtained by treatment of the ovules with 100 μM APM which produced 4.7 diploid plants per 100 ovules. Highest chromosome doubling was found with oryzalin using 1 μM, with trifluralin at 10 μM, and with pronamide at 10 μM producing 2.8, 2.0, and 2.0 diploid plants per 100 ovules, respectively. The APM treatments showed relatively low toxicity on embryo formation which in combination with a high chromosome doubling effect, resulted in up to 89 diploids per 100 plants regenerated. Oryzalin and trifluralin had more severe toxic effects, which reduced embryo formation, thereby lower percentages of chromosome doubled plants were obtained from these treatments. Pronamide had no significant toxic effect but it induced chromosome doubling at lower frequencies. Compared to colchicine, APM seems to be as efficient for chromosome doubling during beet ovule culture, but at molar concentrations 100 times lower than those used for chromosome doubling with colchicine.
    Type of Medium: Electronic Resource
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