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  • 1
    Electronic Resource
    Electronic Resource
    Heterologous transformation of Mucor circinelloides with the Phycomyces blakesleeanus leul gene (1992)
    Springer
    Current genetics 21 (1992), S. 215-223 
    Details
    ISSN: 1432-0983
    Keywords: Zygomycetes ; Leucine ; α-IPM isomerase ; Heterologous transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The leu1 gene of Phycomyces blakesleeanus was isolated within a HindIII-HindIII genomic DNA fragment by heterologous hybridization screening of a cosmid library, making use of the Mucor circinelloides leuA gene as a probe. The complete nucleotide sequence of this fragment reveals a single 2070 bp ORF with no introns, which presents at least 68% homology with that of the leuA gene. The P. blakesleeanus leu1 gene has also been expressed in the M. circinelloides mutant R7B (leu -), which was used to isolate the leuA gene by complementation. The homology with other known sequences shows that the leu1 gene encodes the P. blakesleeanus α-IPM (isopropylmalate) isomerase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336844
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus (1993)
    Springer
    Current genetics 24 (1993), S. 515-519 
    Details
    ISSN: 1432-0983
    Keywords: Phycomyces ; pyrF ; pyrG ; Complementation ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classified by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC 2.4.2.10) activity; the mutant strain belonging to the second group was deficient in orotidine-5′-monophosphate decarboxylase (OMPdecase; EC 4.1.1.23). These mutants are defective in the genes pyrF and pyrG respectively. The results from random spore analysis, tetrad analysis, and gene-centromere distances showed that these two markers are located in linkage group VI, with pyrG being a proximal marker and pyrF a distal one.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351715
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Recombination between ten markers in Phycomyces (1980)
    Springer
    Molecular genetics and genomics 179 (1980), S. 447-452 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Twenty-two sexual crosses between strains of Phycomyces blakesleeanus carrying mutations affecting phototropism (madA, madD, madE), synthesis of carotenoids (carA), auxotrophy (leu-51, nicA, pur-51), and resistance to 5-fluorouracil (fur) were studied; mating type was also included as a marker. Recombination frequencies were obtained among the ten genes involved. Linkage was found between mating type and madE; leu-51 and madA; furA401, furB402 and madD. All other gene combinations tested are unliked.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425475
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  • 4
    Electronic Resource
    Electronic Resource
    A genetic map of Phycomyces blakesleeanus (1987)
    Springer
    Molecular genetics and genomics 210 (1987), S. 69-76 
    Details
    ISSN: 1617-4623
    Keywords: Phycomyces ; Genetic map ; Tetrad analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Complementation tests among Phycomyces auxotrophic strains revealed the existence of four genes with mutants requiring riboflavin, three genes with purine auxotrophs, two with nicotinic acid auxotrophs, and two with lysine auxotrophs. A total of 134 sexual crosses between strains carrying mutations affecting phototropism (madA-madE), carotenoid biosynthesis (carA), auxotrophy (ribA-ribD, purA-purC, lysA and lysB, nicA and nicB, and leuA) and resistance to 5-fluorouracil (furA and furB) were studied; mating type (sex) was also included as a marker. The results from random spore analysis, tetrad analysis, and gene-centromere distances shows that these markers are distributed into 11 linkage groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337760
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  • 5
    Electronic Resource
    Electronic Resource
    A new gene (madI) involved in the phototropic response of Phycomyces (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 148-151 
    Details
    ISSN: 1617-4623
    Keywords: Phycomyces ; Phototropism ; Behavioural mutants ; Photoreceptor ; Complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Only eight genes are known to be involved in the phototropic response of Phycomyces (madA-H). Mutants affected in these genes have played a major role in the analysis of photosensory transduction processes in this system. A set of new mutants isolated by Alvarez et al. (1989) that are unable to bend towards dim unilateral blue light were studied by complementation and recombination. Two of these mutants have mutations in madE, one has a mutation in madF and one is a double madE madF mutant. The three remaining mutants tested did not complement each other and showed positive complementation with strains carrying mutations in the genes madA, madB, and madC, indicating that they carried mutations in a new gene designated madI. Recombination analysis showed that madI is unlinked to madA, madB and madC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315807
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    Paper (German National Licenses)
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