ISSN:
1432-203X
Keywords:
Key words: Transgenic wheat – hygromycin selection – microparticle accelerator – PCR amplification of transgenes.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary. A highly efficient method for stable wheat transformation using hygromicin resistance as a selectable marker is described. Young embryogenic calli growing from immature wheat embryos were transformed using a gunpowder‐driven microparticle accelerator. Transgenic wheat plants were determined by PCR amplification of transgene fragments and confirmed by Southern hybridization, activity of the transgene expression and by analysis of the progeny. The hpt gene was as good as or a better selectable marker than the bar gene with an average efficiency (number of transgenic plants relative to the number of bombarded calli) of 5.5% compared with 2.6% for the bar gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050140
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