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  • 1
    Electronic Resource
    Electronic Resource
    A Study of Four Cases of Pseudoxanthoma Elasticum (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 4 (1977), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Four cases of pseudoxanthoma elasticum (PxE) are reported and the light and electron microscopic appearances described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1977.tb00900.x
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  • 2
    Electronic Resource
    Electronic Resource
    Retinal Vascular Occlusion (1981)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Medicine 32 (1981), S. 101-106 
    Details
    ISSN: 0066-4219
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.me.32.020181.000533
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  • 3
    Electronic Resource
    Electronic Resource
    Detection of Spiroplasma citri in plants and insect hosts by ELISA (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 31 (1982), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Enzyme-linked immunosorbent assay (ELISA) was shown to be a sensitive method for the detection of Spiroplasma citri in plants and insect hosts. S. citri was detected in Vinca rosea less than 1 week after infection by grafting, and reached a peak litre of up to 109 spiroplasmas per gram of tissue in infected shoots and root tips. Multiplication of S. citri in the leafhopper Euscelidius variegatus was also monitored by ELISA. S. citri could be detected in a single insect, showing that this technique is suitable for screening insect populations in the field for potential vectors of Spiroplasma diseases. A method is described for raising a pathogen-specific antiserum from V. rosea infected with S. citri which reacted with cultured S. citri and also with S. citri in plant tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1982.tb01282.x
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro stability and immunoreactivity of the native and recombinant plant food 2S albumins Ber e 1 and SFA-8 (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 33 (2003), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The ability of an intact protein to reach the circulatory system may be a prerequisite to allergenicity and many allergens, particularly those from plant foods, have been found to be consistently more resistant to digestion by pepsin than other proteins.Objective This study assessed the pepsinolytic stability of native 2S albumins from Brazil nut and sunflower seed and their recombinant versions produced in Pichia pastoris. The physicochemical stability of native and recombinant Brazil nut 2S albumins and recombinant sunflower seed 2S albumin was also assessed. The immunoreactivity of native Brazil nut 2S albumin and recombinant 2S albumins was compared using serum from patients allergic to Brazil nuts and animals immunized with native 2S albumins.Methods Digestibility was measured in simulated gastric fluid followed by SDS-PAGE. Circular dichroism spectra were used to analyse unfolding, as proteins were denatured by temperature, pH and guanidinium chloride. Immunoreactivity was assessed by immunoblot, RAST and ELISA.Results Brazil nut 2S albumin was significantly more resistant to proteolytic digestion than other Brazil nut proteins. It was also resistant to thermally and chemically induced denaturation. Equally high resistance to proteolytic digestion was observed with sunflower seed 2S albumin. The recombinant albumins mirrored their native counterparts in stability and immunoreactivity.Conclusion The important food allergen Brazil nut 2S albumin is as stable to digestion as is sunflower seed 2S albumin, whose allergenicity has yet to be determined. The 2S albumins and their recombinant counterparts could not be easily denatured by physicochemical treatments. The results suggest that 2S albumin is the only Brazil nut protein to reach the gut immune system intact. The production of properly folded recombinant proteins will facilitate mechanistic studies as well as diagnostic testing and antigen-based therapies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2003.01736.x
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  • 5
    Electronic Resource
    Electronic Resource
    Intra-ocular pressure changes during induction of anaesthesia and tracheal intubation (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Anaesthesia 43 (1988), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Intra-ocular pressure was measured during induction of anaesthesia with propofol (n = 40) or thiopentone (n = 40) followed by vecuronium to facilitate tracheal intubation which was carried out 3 minutes after the administration of relaxant. The average induction doses were 2.15 and 4.83 mg/kg for propofol and thiopentone, respectively. Half the patients in each group received a supplementary dose of the same induction agent (propofol 1.0 mg/kg or thiopentone 20 mg/kg) prior to intubation. Both propofol and thiopentone produced a significant reduction in intra-ocular pressure which decreased further after administration of vecuronium as well as the second smaller dose of the induction agents. Intra-ocular pressure prior to intubation was lower in the two propofol groups in comparison to the corresponding thiopentone groups. Intubation was associated with an increase in intra-ocular pressure but it still remained significantly below the baseline values except in the group given one dose of thiopentone. Supplementary doses of inducation agents before intubation attenuated the increase in intra-ocular pressure. Propofol was significantly more effective this respect and this group showed the lowest intra-ocular pressure throughout the study period. However administration of propofol resulted in a 30% incidence of pain on injection and a decrease in systolic arterial pressure of more than 30% in about half the patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2044.1988.tb09071.x
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  • 6
    Electronic Resource
    Electronic Resource
    Pathogenic mechanisms of mycoplasmas (1979)
    [s.l.] : Nature Publishing Group
    Nature 277 (1979), S. 268-268 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] MYCOPLASMAS are a diverse group of wall-less prokaryotes. They have been implicated as possible causal agents in a multitude of diseases in animals, plants and insects but have been demonstrated to be causative in relatively few instances and in only a handful of cases is there any information ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/277268a0
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  • 7
    Electronic Resource
    Electronic Resource
    Heterologous protein secretion by Aspergillus niger growing in submerged culture as dispersed or aggregated mycelia (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 157-160 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The secreted production of a heterologous enzyme, hen egg-white lysozyme, by Aspergillus niger was studied in shake flasks containing media of different initial viscosities. Raising the viscosity of the medium by addition of polyvinylpyrrolidone (PVP) brought about a transition in the form of growth from aggregated mycelia (pellets) to dispersed mycelia. The specific yield of lysozyme in cultures containing an initial concentration of 5% (w/v) starch was 8 mg lysozyme/g dry weight. Addition of 2% (w/v) PVP to the medium resulted in a specific yield of 14 mg lysozyme/g dry weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050534
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  • 8
    Electronic Resource
    Electronic Resource
    Heterologous protein secretion by Aspergillus niger growing in submerged culture as dispersed or aggregated mycelia (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 157-160 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The secreted production of a heterologous enzyme, hen egg-white lysozyme, by Aspergillus niger was studied in shake flasks containing media of different initial viscosities. Raising the viscosity of the medium by addition of polyvinylpyrrolidone (PVP) brought about a transition in the form of growth from aggregated mycelia (pellets) to dispersed mycelia. The specific yield of lysozyme in cultures containing an initial concentration of 5% (w/v) starch was 8 mg lysozyme/g dry weight. Addition of 2% (w/v) PVP to the medium resulted in a specific yield of 14 mg lysozyme/g dry weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00164495
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  • 9
    Electronic Resource
    Electronic Resource
    Altered endothelin-1 induced contraction and second messenger generation in bovine retinal microvascular pericytes cultured in high glucose medium (1994)
    Springer
    Diabetologia 37 (1994), S. 36-42 
    Details
    ISSN: 1432-0428
    Keywords: Retinal microvascular pericytes ; hyperglycaemia ; endothelin-1 ; inositol (1,4,5) trisphosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of simulated hyperglycaemia on bovine retinal pericytes was studied following culture of these cells for 10 days under normal (5 mmol/l) and elevated (25 mmol/l) glucose conditions in the absence of endothelial cells. Pericytes cultured under high ambient glucose exhibited both a delayed and reduced contractile response following stimulation with endothelin-1. Stimulation with 10−7mol/l endothelin-1 for 30 s caused significant contraction in cells grown in both 5 mmol/l and 25 mmol/l glucose. The former also contracted significantly with 10−8mol/l endothelin-1. Further, at all concentrations tested, statistical comparison of the time course of contraction showed a significant difference (p〈0.02) in the reduction of planimetric surface area between the two cell groups. Since neither binding of endothelin-1 nor the number of receptors for this peptide were significantly different (p〉0.1) between bovine retinal pericytes grown for 10 days under normo- or hyperglycaemic conditions, it became apparent that the altered contractility in bovine retinal pericytes following culture in high glucose must be due to post-binding intracellular disturbance(s). Indeed, both basal and 15 s post-stimulation with 10−8 mol/l endothelin-1, levels of inositol trisphosphate were significantly reduced (p〈0.05 and p〈0.02, respectively) in pericytes cultured for 10 days in 25 mmol/l glucose. These results show that endothelialindependent alterations in contractility of pericytes occur when they are grown in conditions which simulate hyperglycaemia. The results also suggest that the observed attenuation in response to endothelin-1 stimulation evident in pericytes grown under simulated hyperglycaemic conditions is not due to alterations in peptide binding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428775
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  • 10
    Electronic Resource
    Electronic Resource
    Increased endocytosis in retinal vascular endothelial cells grown in high glucose medium is modulated by inhibitors of nonenzymatic glycosylation (1995)
    Springer
    Diabetologia 38 (1995), S. 1271-1275 
    Details
    ISSN: 1432-0428
    Keywords: Key words Endocytosis ; high glucose ; nonenzymatic glycosylation ; endothelium.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We sought to determine if hyperglycaemia is responsible for increased retinal vascular endothelial-cell (RVEC) endocytosis in diabetes and to assess the role of nonenzymatic glycosylation in mediation of this novel endothelial-cell pathology. RVECs were propagated in media containing either 5 or 25 mmol/l glucose for up to 10 days after which they were exposed to the protein tracer horseradish peroxidase for 30 min. The level of RVEC endocytosis was quantified in intact cell monolayers by electron microscopic stereology, and in cell lysates by a simple spectrophotometric method. The effect of the nonenzymatic glycosylation inhibitors, aminoguanidine and d-lysine, on high-glucose medium induced changes in RVEC endocytosis was tested by inclusion of these agents in the culture medium. RVECs exposed to 25 mmol/l glucose showed a stepwise increase in endocytosis of horseradish peroxidase culminating in a two- to threefold increase after 10 days. Endocytosis returned to normal levels after a further 10 days in 5 mmol/l glucose medium. The increase in RVEC endocytosis was markedly reduced, but not completely normalised, by aminoguanidine and d-lysine. Exposure of cultured RVECs to 25 mmol/l glucose causes an increase in endocytosis of similar magnitude to that experienced by RVEC in early diabetes, and implicates hyperglycaemia in the latter situation. A significant component of the increase in RVEC endocytosis appears to be mediated by nonenzymatic glycosylation. [Diabetologia (1995) 38: 1271–1275]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401758
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